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EC number: 249-769-6 | CAS number: 29676-71-9
- Life Cycle description
- Uses advised against
- Endpoint summary
- Appearance / physical state / colour
- Melting point / freezing point
- Boiling point
- Density
- Particle size distribution (Granulometry)
- Vapour pressure
- Partition coefficient
- Water solubility
- Solubility in organic solvents / fat solubility
- Surface tension
- Flash point
- Auto flammability
- Flammability
- Explosiveness
- Oxidising properties
- Oxidation reduction potential
- Stability in organic solvents and identity of relevant degradation products
- Storage stability and reactivity towards container material
- Stability: thermal, sunlight, metals
- pH
- Dissociation constant
- Viscosity
- Additional physico-chemical information
- Additional physico-chemical properties of nanomaterials
- Nanomaterial agglomeration / aggregation
- Nanomaterial crystalline phase
- Nanomaterial crystallite and grain size
- Nanomaterial aspect ratio / shape
- Nanomaterial specific surface area
- Nanomaterial Zeta potential
- Nanomaterial surface chemistry
- Nanomaterial dustiness
- Nanomaterial porosity
- Nanomaterial pour density
- Nanomaterial photocatalytic activity
- Nanomaterial radical formation potential
- Nanomaterial catalytic activity
- Endpoint summary
- Stability
- Biodegradation
- Bioaccumulation
- Transport and distribution
- Environmental data
- Additional information on environmental fate and behaviour
- Ecotoxicological Summary
- Aquatic toxicity
- Endpoint summary
- Short-term toxicity to fish
- Long-term toxicity to fish
- Short-term toxicity to aquatic invertebrates
- Long-term toxicity to aquatic invertebrates
- Toxicity to aquatic algae and cyanobacteria
- Toxicity to aquatic plants other than algae
- Toxicity to microorganisms
- Endocrine disrupter testing in aquatic vertebrates – in vivo
- Toxicity to other aquatic organisms
- Sediment toxicity
- Terrestrial toxicity
- Biological effects monitoring
- Biotransformation and kinetics
- Additional ecotoxological information
- Toxicological Summary
- Toxicokinetics, metabolism and distribution
- Acute Toxicity
- Irritation / corrosion
- Sensitisation
- Repeated dose toxicity
- Genetic toxicity
- Carcinogenicity
- Toxicity to reproduction
- Specific investigations
- Exposure related observations in humans
- Toxic effects on livestock and pets
- Additional toxicological data
Toxicity to aquatic algae and cyanobacteria
Administrative data
- Endpoint:
- toxicity to aquatic algae and cyanobacteria
- Type of information:
- experimental study
- Adequacy of study:
- key study
- Reliability:
- 1 (reliable without restriction)
- Rationale for reliability incl. deficiencies:
- guideline study
Data source
Reference
- Reference Type:
- study report
- Title:
- Unnamed
- Year:
- 2 011
- Report date:
- 2011
Materials and methods
Test guidelineopen allclose all
- Qualifier:
- according to guideline
- Guideline:
- OECD Guideline 201 (Freshwater Alga and Cyanobacteria, Growth Inhibition Test)
- Deviations:
- no
- Qualifier:
- according to guideline
- Guideline:
- EU Method C.3 (Algal Inhibition test)
- Deviations:
- no
- GLP compliance:
- yes
Test material
- Reference substance name:
- 2-aminothiazol-4-acetic acid
- EC Number:
- 249-769-6
- EC Name:
- 2-aminothiazol-4-acetic acid
- Cas Number:
- 29676-71-9
- Molecular formula:
- C5H6N2O2S
- IUPAC Name:
- (2-amino-1,3-thiazol-4-yl)acetic acid
- Test material form:
- solid: particulate/powder
Constituent 1
Sampling and analysis
- Analytical monitoring:
- yes
- Details on sampling:
- - Concentrations: 10, 18, 32, 56 and 100 mg/L
- Sampling method: Measured at 0 and 72 hours using HPLC.
- Sample storage conditions before analysis: Not specified.
Test solutions
- Vehicle:
- no
- Details on test solutions:
- PREPARATION AND APPLICATION OF TEST SOLUTION (especially for difficult test substances)
A primary stock solution of the test substance with a nominal concentration of 100 mg/L was prepared. This stock was then stirred for 5 minutes and then ultrasonicated for 10 minutes after which it was observed to be a clear and colourless solution. this stock was then used directly as the 100 mg/L test solution and was serially diluted in culture medium to provide the other test solutions. All test solutions and the culture medium control were observed to be clear and colourless solutions.
The appropriate test solution (100 mL) was then dispensed to each test and blank vessel.
Test organisms
- Test organisms (species):
- Pseudokirchneriella subcapitata (previous names: Raphidocelis subcapitata, Selenastrum capricornutum)
- Details on test organisms:
- TEST ORGANISM
- Common name: Green algae
- Strain: CCAP 278/4
- Source (laboratory, culture collection): Sourced from laboratory cultures maintained under axenic conditions.
- Age of inoculum (at test initiation): 3 day old cuture
- Method of cultivation: Not specified
ACCLIMATION
- Acclimation period: Not specified
- Culturing media and conditions (same as test or not): Same as test
- Any deformed or abnormal cells observed: Not specified
Study design
- Test type:
- static
- Water media type:
- freshwater
- Limit test:
- no
- Total exposure duration:
- 72 h
Test conditions
- Hardness:
- Not specified
- Test temperature:
- 22 +/- 2°C
- pH:
- pH values at 0 hours and 72 hours
Control: 7.1 to 7.5.
10 mg/L: 6.6 to 7.3
18 mg/L: 6.2 to 7.5
32 mg/L: 5.9 (6.9 after adjustment) to 7.5
56 mg/L: 5.5 (7.0 after adjustment) to 7.4
100 mg/L: 5.2 (7.0 after adjustment) to 7.4 - Dissolved oxygen:
- Not specified
- Salinity:
- Not specified
- Conductivity:
- Not specified
- Nominal and measured concentrations:
- Nominal: 0, 10, 18, 32, 56 and 100 mg/L.
Measured: 0, 10, 18, 32 57 and 100 mg/L. - Details on test conditions:
- TEST SYSTEM
- Test vessel: Glass conical flasks
- Type (delete if not applicable): open (stoppered with foam bungs)
- Material, size, headspace, fill volume: 250 mL volume filled with 100 mL of solutions
- Aeration: No
- Initial cells density: 5000 cells/mL
- Control end cells density: 634000 cells/mL
- No. of organisms per vessel: NA
- No. of vessels per concentration (replicates): 3
- No. of vessels per control (replicates): 6
- No. of vessels per vehicle control (replicates): NA
GROWTH MEDIUM
- Standard medium used: yes as per OECD guidelines
TEST MEDIUM / WATER PARAMETERS
- Standard
OTHER TEST CONDITIONS
- Sterile test conditions: yes/no
- Adjustment of pH: Yes
- Photoperiod: Continuous
- Light intensity and quality: 6910 +/- 7% Lux
EFFECT PARAMETERS MEASURED (with observation intervals if applicable) : Cell density used to determine growth rate and yield with microscopic evaluation also performed.
- Determination of cell concentrations: Coulter counter counting at a lower threshold equivalent spherical diameter of approximately 2.3 µm.
TEST CONCENTRATIONS
- Spacing factor for test concentrations: Standard as per guidelines.
- Justification for using less concentrations than requested by guideline: NA
- Range finding study: Not specified - Reference substance (positive control):
- not specified
Results and discussion
Effect concentrationsopen allclose all
- Duration:
- 72 h
- Dose descriptor:
- EC50
- Effect conc.:
- > 100 mg/L
- Nominal / measured:
- nominal
- Conc. based on:
- test mat.
- Basis for effect:
- growth rate
- Duration:
- 72 h
- Dose descriptor:
- EC50
- Effect conc.:
- > 100 mg/L
- Nominal / measured:
- nominal
- Conc. based on:
- test mat.
- Basis for effect:
- other: yield
- Duration:
- 72 h
- Dose descriptor:
- NOEC
- Effect conc.:
- 56 mg/L
- Nominal / measured:
- nominal
- Conc. based on:
- test mat.
- Basis for effect:
- growth rate
- Duration:
- 72 h
- Dose descriptor:
- NOEC
- Effect conc.:
- 56 mg/L
- Nominal / measured:
- nominal
- Conc. based on:
- test mat.
- Basis for effect:
- other: yield
- Details on results:
- - Exponential growth in the control (for algal test): yes
- Observation of abnormalities (for algal test): None
- Unusual cell shape: None
- Colour differences: None
- Flocculation: None
- Adherence to test vessels: None
- Aggregation of algal cells: None
- Any stimulation of growth found in any treatment: Yes at 32 mg/L (104% of control)
- Any observations (e.g. precipitation) that might cause a difference between measured and nominal values: None
- Effect concentrations exceeding solubility of substance in test medium: None - Results with reference substance (positive control):
- Not specified
- Reported statistics and error estimates:
- The data for cell counts obtained at 24, 48 and 72 hours was entered into electronic data files and statistically analysed using an in-house software package. Appropriate procedures were applied to test for significant differences (p < 0.05) between the control and test concentrations. Where possible, the EC50, EC20 and EC10 values and teh associated 95% confidence intervals were determined using the US EPA program ICPIN.
Applicant's summary and conclusion
- Validity criteria fulfilled:
- yes
- Remarks:
- Validity criteria with regards to controls met
- Conclusions:
- Under the conditions of the study the 72 hour EC50 values for growth rate and yield were higher than the upper concentration tested (100 mg/L). Analytical verification was conducted providing near nominal results on all occassions.
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