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Ecotoxicological information

Short-term toxicity to aquatic invertebrates

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Administrative data

Endpoint:
short-term toxicity to aquatic invertebrates
Type of information:
experimental study
Adequacy of study:
weight of evidence
Study period:
1989
Reliability:
2 (reliable with restrictions)
Rationale for reliability incl. deficiencies:
test procedure in accordance with national standard methods with acceptable restrictions

Data source

Reference
Reference Type:
study report
Title:
Unnamed
Year:
1989
Report Date:
1989

Materials and methods

Test guideline
Qualifier:
equivalent or similar to
Guideline:
EU Method C.2 (Acute Toxicity for Daphnia)
GLP compliance:
not specified

Test material

Reference
Name:
Unnamed
Type:
Constituent
Test material form:
solid

Sampling and analysis

Analytical monitoring:
not specified

Test solutions

Vehicle:
no
Details on test solutions:
- Preparation of a stock solution of 120 mg/L in water

Test organisms

Test organisms (species):
Daphnia magna
Details on test organisms:
TEST ORGANISM
- Common name: Daphnia magna Strauss
- Strain: not available
- Source: Bayer AG Leverkusen
- Age at study initiation (mean and range, SD): 6-24 hrs
- Food type: Scenedesmus Subspicatus (ad libitum)
- Frequency: Daily
- Health during acclimation (any mortality observed): none

- Cultivation: daily feeding with Scenedesmus Subspicatus (ad libitum) (SOP 4003)
- Water : "aufbereitetes Leverkusener Trinkwasser" of drinking quality.
- Medical pre-treatment: none


Study design

Test type:
static
Water media type:
freshwater
Limit test:
no
Total exposure duration:
24 h

Test conditions

Nominal and measured concentrations:
Nominal test concentrations: 0.32, 1.0, 3.2, 10, 31.6 and 100 mg/L
Details on test conditions:
- Test vessels (cylindrical) : 4 cm diameter and 7 cm high
- Volume of the test medium: 20 mL
- Ventilation: none
- Reference substance: Potassium dichromate
- Measurement and numerical values during the test: Oxygen, pH, Temperature of water, Number of immobile Daphnia
Reference substance (positive control):
yes
Remarks:
potassium dichromate

Results and discussion

Effect concentrationsopen allclose all
Duration:
24 h
Dose descriptor:
EC0
Effect conc.:
0.32 mg/L
Nominal / measured:
nominal
Conc. based on:
test mat.
Duration:
24 h
Dose descriptor:
EC50
Effect conc.:
0.57 mg/L
Nominal / measured:
nominal
Conc. based on:
test mat.
Duration:
24 h
Dose descriptor:
EC100
Effect conc.:
1 mg/L
Nominal / measured:
nominal
Conc. based on:
test mat.
Results with reference substance (positive control):
- Results with reference substance valid? yes


Applicant's summary and conclusion

Validity criteria fulfilled:
not specified
Conclusions:
The EC50 (24 h) of the substance to Daphnia magna was determined to be 0.57 mg/L.
Executive summary:

The 24–hr-acute toxicity of the test item to Daphnia magna was studied at nominal concentrations of 0.32, 1.0, 3.2, 10, 31.6 and 100 mg/L. Test conditions were similar to the standard test guideline EU Method C.2 (Acute toxicity for Daphnia). The 24– hour EC50was determined to be 0.57 mg/L.