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Ecotoxicological information

Short-term toxicity to aquatic invertebrates

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Administrative data

Endpoint:
short-term toxicity to aquatic invertebrates
Type of information:
other: Publication
Adequacy of study:
weight of evidence
Study period:
48 hours
Reliability:
2 (reliable with restrictions)
Rationale for reliability incl. deficiencies:
study well documented, meets generally accepted scientific principles, acceptable for assessment
Cross-reference
Reason / purpose:
read-across: supporting information

Data source

Reference
Reference Type:
review article or handbook
Title:
Unnamed
Year:
2008
Report Date:
2008

Materials and methods

Test guideline
Qualifier:
according to
Guideline:
OECD Guideline 202 (Daphnia sp. Acute Immobilisation Test)
Version / remarks:
OECD. 202 Daphnia sp. Acute Immobilisation Test OECD Guidelines for Testing of
Chemicals. Paris; 2004.
Deviations:
not specified
GLP compliance:
not specified

Test material

Reference
Name:
Unnamed
Type:
Constituent
Specific details on test material used for the study:
The amphoteric surfactants tested were: three alkyl betaines (decyl dimethyl-,dodecyl dimethyl- and tetradecyl dimethyl betaine; C10-Bet, C12-Bet and C14-Bet,
respectively). The alkyl betaines were supplied by Albright and Wilson Ltd.
The cetyl betaine substance, subject of this registration, is a predominently C16 alkyl betaine (97%).
Use of data from this scientific paper is justified since the C14 alkyl betaine used for the investigation of the environmental effects is similar to this substance, only differeing in C chain length.

Test organisms

Test organisms (species):
Daphnia magna
Details on test organisms:
D. magna, laboratory bred, not more than 24 h old were used in this test

Study design

Test type:
static
Water media type:
freshwater
Total exposure duration:
48 h

Test conditions

Hardness:
250 mg/l as CaCO3
Test temperature:
20C
pH:
8
Details on test conditions:
Testswere performed in the dark at 20 °C. Twenty daphnia, divided into four batches of five animals each,was used at each test concentration. For each surfactant, ten concentrations in a geometric series
were tested in the concentration range first established in a preliminary test. The percentage immobility at 48 h was plotted against concentration on logarithmic probability paper and a linear relationship was obtained.
The ProbitMethodwas employed as the statistical procedure to determine the IC50 (the estimated concentration to immobilise 50% of the daphnia after 48 h exposure) and the corresponding 95% confidence interval (CI).
Reference substance (positive control):
not specified

Results and discussion

Effect concentrations
Duration:
48 h
Dose descriptor:
EC50
Effect conc.:
9.8 mg/L
Nominal / measured:
meas. (arithm. mean)
Conc. based on:
test mat.
Basis for effect:
mobility
Details on results:
The conclusion of this study was that Alkyl betaines (C12-C14) were found to be Moderately toxic to aqautic life.
However, in addition, the biodegradation curves obtained for the alkyl betaines tested showed a high
biodegradation level (>60%) was reached by the end of the test according to the requirements of OECD 202

Applicant's summary and conclusion