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EC number: 224-320-7
CAS number: 4306-88-1
adsorption coefficient of the test item on solid and on sewage sludge (KOC)
was determined by HPLC according to EC method C.19 (440/2008) and OECD
guideline 121, under GLP. The analysis was performed on an analytical
column packed with a commercially available cyanopropyl solid phase
containing lipophilic and polar moieties. A moderately polar stationary
phase based on a silica matrix was used (EC 50/4.6 Nucleodur 100-5 CN-RP
150 x 4.6 mm; 5µm).
A mixture of 55% methanol / 45 % ultra-pure grade water was used as
mobile phase and the column temperature was 25°C±1°C.
The study reports the following values: 6.55-7.20 of which the average
is 6.93 (key value). The range of 6.55-7.20 is above the determination
limit of the method (log KOC5) and above the highest
reference standard 5.63). The above values were hence extrapolated. The
determined log KOCof two runs is within a range of±0.25
log units (absolute deviation). Hence the requirements of OECD guideline
121 concerning the quality criteria are fulfilled.
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