2,6-di-tert-butyl-4-nonylphenol

Biodegradation in water: screening tests

Administrative data

Endpoint:

biodegradation in water: inherent biodegradability

Type of information:

experimental study

Adequacy of study:

key study

Study period:

11.5.95 - 8.6.95

Reliability:

2 (reliable with restrictions)

Rationale for reliability incl. deficiencies:

other: non-GLP

Remarks:

non-GLP

Justification for type of information:

The study is suitable to fullfill data requests.

Data source

Reference

Reference Type:

study report

Title:

Unnamed

Year:

1995

Report date:

1995

Materials and methods

Test guideline

Qualifier:

according to guideline

Guideline:

OECD Guideline 302 C (Inherent Biodegradability: Modified MITI Test (II))

Version / remarks:

1981

GLP compliance:

no

Test material

Test material information

Constituent 1

Reference substance name:

2,6-di-tert-butyl-4-nonylphenol

EC Number:

224-320-7

EC Name:

2,6-di-tert-butyl-4-nonylphenol

Cas Number:

4306-88-1

Molecular formula:

C23H40O

IUPAC Name:

2,6-di-tert-butyl-4-nonylphenol

Details on test material:

Isonox 232 (Phenol, 2,6-bis (1,1-dimethylethyl)-4-nonyl)
Clear colorless liquid
Storage: In original container at room temperature
Batch number not provided in report

Study design

Oxygen conditions:

aerobic

Inoculum or test system:

activated sludge, non-adapted

Details on inoculum:

100 mg dry mass/L non-adapted microorganisms from 10 different sewage treatment plants throughout England. The mixed sludge was fed daily with a 0.1% synthetic sewage and maintained on constant aeration at 25±2°C until use.

Duration of test (contact time):

28 d

Initial test substance concentration

Initial test substance concentration 1

Initial conc.:

30 mg/L

Based on:

test mat.

Initial test substance concentration 2

Initial conc.:

3.13 mg/L

Based on:

ThOD

Parameter followed for biodegradation estimation

Parameter followed for biodegradation estimation:

O2 consumption

Reference substance

Reference substance:

aniline

Results and discussion

Test performance:

The test was conducted in the dark. The results of the reference substance show the suitability of inoculum and culture conditions.

% Degradation

Key result

Parameter:

% degradation (O2 consumption)

Value:

31

Sampling time:

28 d