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Description of key information

Short term toxicity to aq. invertebrates:

Based on the prediction done using the OECD QSAR toolbox version 3.4 with log kow as the primary descriptor and considering the six closest read across substances, toxicity on daphnia magna was predicted hexasodium 4,4'-{ethene-1,2-diylbis[(3-sulfonato-4,1-phenylene)imino (6-chloro-1,3,5-triazine- 4,2-diyl)imino]}bis{6-[(5-carbamoyl-1-ethyl-2-hydroxy-4-methyl-6-oxo-1,6-dihydropyridin-3-yl) diazenyl]benzene-1,3-disulfonate} (CAS no. 68991 -98 -0). Based on the intoxication of test organism the EC50 value was estimated to be 418.3 mg/l when hexasodium 4,4'-{ethene-1,2-diylbis [(3-sulfonato-4,1-phenylene)imino (6-chloro-1,3,5-triazine- 4,2-diyl)imino]}bis{6-[(5-carbamoyl-1-ethyl-2-hydroxy-4-methyl-6-oxo-1,6-dihydropyridin-3-yl) diazenyl]benzene-1,3-disulfonate} exposed to daphnia magna for 48 hrs. Based on this value it can be concluded that the substance hexasodium 4,4'-{ethene-1,2-diylbis[(3-sulfonato-4,1-phenylene)imino (6-chloro-1,3,5-triazine- 4,2-diyl)imino]}bis{6-[(5- carbamoyl-1-ethyl-2-hydroxy-4-methyl-6-oxo-1,6-dihydropyridin-3-yl) diazenyl]benzene-1,3-disulfonate} (68991 -98 -0) is considered to be nontoxic to aquatic environment and cannot classified as per the criteria mentioned in CLP regulation.  

    

Toxicity to aq. algae and cyanobacteria:

Based on the prediction done using the OECD QSAR toolbox version 3.4 with log kow as the primary descriptor and considering the five closest read across substances, toxicity on Pseudokirchneriella subcapitata (previous names: Raphidocelis subcapitata, Selenastrum capricornutum) was predicted for hexasodium 4,4'-{ethene-1,2-diylbis[(3-sulfonato-4,1-phenylene)imino(6-chloro-1,3,5-triazine- 4,2-diyl)imino]}bis{6-[(5-carbamoyl-1-ethyl-2-hydroxy-4-methyl-6-oxo-1,6-dihydropyridin-3-yl) diazenyl]benzene-1,3-disulfonate} (CAS no. 68991 -98 -0). Based on the growth rate inhibition of algae, the EC50 value was estimated to be 107.81 mg/l when hexasodium 4,4'-{ethene-1,2-diylbis[(3-sulfonato-4,1-phenylene)imino(6-chloro-1,3,5-triazine- 4,2-diyl)imino]}bis{6-[(5-carbamoyl-1-ethyl-2-hydroxy- 4-methyl- 6-oxo-1,6-dihydropyridin-3-yl) diazenyl]benzene-1,3-disulfonate} exposed to Pseudokirchneriella subcapitata for 72hrs. Based on this value it can be concluded that the substance hexasodium 4,4'-{ethene-1,2-diylbis[(3-sulfonato-4,1-phenylene)imino(6-chloro-1,3,5-triazine- 4,2-diyl)imino]}bis{6-[(5-carbamoyl-1-ethyl-2-hydroxy-4-methyl-6-oxo-1,6-dihydropyridin-3-yl) diazenyl]benzene-1,3-disulfonate} (CAS no. 68991 -98 -0) is considered to be nontoxic to aquatic environment and cannot classified as per the criteria mentioned in CLP regulation.            

Additional information

Summarized result for the determination of nature of chemical hexasodium 4,4'-{ethene-1,2-diylbis[(3-sulfonato-4,1-phenylene)imino (6-chloro-1,3,5-triazine- 4,2-diyl)imino]}bis{6-[(5-carbamoyl-1-ethyl -2-hydroxy-4-methyl-6 -oxo-1,6-dihydropyridin- 3-yl) diazenyl]benzene-1,3-disulfonate} (CAS no. 68991 -98 -0) on the growth and other biological and physical activity of aquatic invertebrates, algae and cyanobacteria when chemical comes in contact with test organisms, by considering the data for target as well as structurally and functionally similar read across chemicals which are as follows:  

 

Short term toxicity to aq. invertebrates:

Based on the various predicted data for the target chemical and experimental data for structurally and functionally similar read across chemicals study have been reviewed to determine the toxic nature of target chemical hexasodium 4,4'-{ethene-1,2-diylbis[(3-sulfonato-4,1-phenylene)imino (6-chloro-1,3,5-triazine- 4,2-diyl)imino]}bis{6-[(5-carbamoyl-1-ethyl-2-hydroxy-4-methyl-6 -oxo-1,6-dihydropyridin- 3-yl) diazenyl]benzene-1,3-disulfonate} (CAS no. 68991 -98 -0) on the mobility of aquatic invertebrates. The studies are as mentioned below:  

 

In the first predicted data for the target chemical using the OECD QSAR toolbox version 3.4 with log kow as the primary descriptor and considering the six closest read across substances, toxicity on daphnia magna was predicted hexasodium 4,4'-{ethene-1,2-diylbis[(3-sulfonato-4,1-phenylene)imino (6-chloro-1,3,5-triazine- 4,2-diyl)imino]}bis{6-[(5-carbamoyl-1-ethyl-2-hydroxy-4-methyl-6 -oxo-1,6-dihydropyridin- 3-yl) diazenyl]benzene-1,3-disulfonate} (CAS no. 68991 -98 -0). Based on the intoxication of test organism the EC50 value was estimated to be 418.3 mg/l when hexasodium 4,4'-{ethene-1,2-diylbis [(3-sulfonato-4,1-phenylene)imino (6-chloro-1,3,5-triazine- 4,2-diyl)imino]}bis{6-[(5-carbamoyl-1-ethyl-2-hydroxy-4-methyl-6-oxo-1,6-dihydropyridin-3-yl) diazenyl]benzene-1,3-disulfonate} exposed to daphnia magna for 48 hrs. Based on this value it can be concluded that the substance hexasodium 4,4'-{ethene-1,2-diylbis[(3-sulfonato-4,1-phenylene)imino (6-chloro-1,3,5-triazine- 4,2-diyl)imino]}bis{6-[(5- carbamoyl-1-ethyl-2-hydroxy-4-methyl-6-oxo-1,6-dihydropyridin-3-yl) diazenyl]benzene-1,3-disulfonate} (68991 -98 -0) is considered to be nontoxic to aquatic environment and cannot classified as per the criteria mentioned in CLP regulation.  

 

First predicted study was supported by the second experimental supporting study for the read across chemical 4-methoxyphenylacetic acid (12225-21-7) from ABITEC report. Determination of the inhibition of the mobility of daphnids was carried out with the substance Aluminium,4,5-dihydro-5-oxo-1-(4-sulfophenyl)-4-[(4-sulfophenyl)azo]-1H-pyrazole- 3-carboxylic acid complex (CAS No12225-21-7) according to OECD Guideline 202. The test substance was tested at the concentrations 0, 50, 70, 100, 140, 200 mg/L. Effects on immobilisation were observed for 48 hours. The median effective concentration (EC50) for the test substance Aluminium, 4,5-dihydro-5-oxo-1-(4-sulfophenyl)-4-[(4-sulfophenyl)azo]-1H-pyrazole-3-carboxylic acid complex (CAS No12225-21-7) in Daphnia magna was determined to be 360.4 mg/L for immobilisation effects. This value indicates that the Aluminium,4,5-dihydro-5-oxo-1-(4-sulfophenyl)-4-[(4-sulfophenyl)azo]-1H-pyrazole -3- carboxylic acid complex (CAS No 12225-21-7) is likely to be non-hazardous to aquatic invertebrates and can be classified as "not classified" as per the CLP criteria.

 

Based on the predicted data for the target chemical (from OECD QSAR) and for the read across chemical from experimental lab reports (ABITEC report), it can be concluded that the substance

hexasodium 4,4'-{ethene-1,2-diylbis[(3-sulfonato-4,1-phenylene)imino (6-chloro-1,3,5-triazine- 4,2-diyl)imino]}bis{6-[(5-carbamoyl-1-ethyl-2-hydroxy-4-methyl-6 -oxo-1,6-dihydropyridin- 3-yl) diazenyl] benzene-1,3-disulfonate} (CAS no. 68991 -98 -0) is considered to be not toxic to aquatic environment (aquatic invertebrates) and cannot be classified as toxic as per the criteria mentioned in CLP regulation. 

 

Toxicity to aq. algae and cyanobacteria:

Based on the various predicted data for the target chemical and experimental data for structurally and functionally similar read across chemicals study have been reviewed to determine the toxic nature of target chemical hexasodium 4,4'-{ethene-1,2-diylbis[(3-sulfonato-4,1-phenylene)imino (6-chloro-1,3,5-triazine- 4,2-diyl)imino]}bis{6-[(5-carbamoyl-1-ethyl-2-hydroxy-4-methyl-6-oxo-1,6-dihydropyridin-3-yl) diazenyl]benzene-1,3-disulfonate} (CAS no. 68991 -98 -0) on the growth of aquatic algae and cyanobacteria. The studies are as mentioned below:  

 

In the first predicted data for the target chemical using the OECD QSAR toolbox version 3.4 with log kow as the primary descriptor and considering the five closest read across substances, toxicity on Pseudokirchneriella subcapitata (previous names: Raphidocelis subcapitata, Selenastrum capricornutum) was predicted for hexasodium 4,4'-{ethene-1,2-diylbis[(3-sulfonato-4,1-phenylene)imino (6-chloro-1,3,5-triazine- 4,2-diyl)imino]}bis{6-[(5-carbamoyl-1-ethyl-2-hydroxy-4-methyl-6-oxo-1,6-dihydropyridin-3-yl) diazenyl]benzene-1,3-disulfonate} (CAS no. 68991 -98 -0). Based on the growth rate inhibition of algae, the EC50 value was estimated to be 107.81 mg/l when hexasodium 4,4'-{ethene-1,2-diylbis[(3-sulfonato-4,1-phenylene)imino(6-chloro-1,3,5-triazine- 4,2-diyl)imino]}bis{6-[(5-carbamoyl-1- ethyl-2-hydroxy- 4-methyl- 6-oxo-1,6-dihydropyridin-3-yl) diazenyl]benzene-1,3-disulfonate} exposed to Pseudokirchneriella subcapitata for 72hrs. Based on this value it can be concluded that the substance hexasodium 4,4'-{ethene-1,2-diylbis[(3-sulfonato-4,1-phenylene)imino(6-chloro-1,3,5-triazine- 4,2-diyl)imino]}bis{6-[(5-carbamoyl-1-ethyl-2-hydroxy-4-methyl-6-oxo- 1,6-dihydropyridin-3-yl) diazenyl]benzene-1,3-disulfonate} (CAS no. 68991 -98 -0) is considered to be nontoxic to aquatic environment and cannot classified as per the criteria mentioned in CLP regulation.            

 

First predicted study was supported by the second experimental supporting study for the read across chemical 4-methoxyphenylacetic acid (12225-21-7) from ABITEC report. The effect of test item Aluminium, 4,5-dihydro-5-oxo-1-(4-sulfophenyl)-4-[(4-sulfophenyl)azo]-1Hpyrazole-3-carboxylic acid complex, (CAS No. 12225-21-7) was studied on the growth of fresh water green alga Chlorella vulgaris. The study was conducted following OECD guideline 201- Alga, growth inhibition test. The test concentration chosen for the study were 6.25 mg/L,12.5 mg/L,25 mg/L,50 mg/L,100 mg/L,200 mg/L. The test concentrations were prepared using stock solution of the test item using mineral media. The green alga was exposed to the test concentration for a period of 72 hours to observe average specific growth rate and % growth inhibition under the effect of the test item. EC50 calculated graphically through probit analysis was observed to be >200 mg/L. It can be concluded from the value that the Aluminium, 4,5-dihydro-5-oxo-1-(4-sulfophenyl)-4-[(4-sulfophenyl)azo]-1Hpyrazole-3-carboxylic acid complex, (CAS No. 12225-21-7) is not toxic to the aquatic algae and can be considered as "not classified" as per the classification criteria for aquatic environment.

 

Based on the predicted data for the target chemical (from OECD QSAR) and for the read across chemical from experimental lab reports (ABITEC report), it can be concluded that the substance

hexasodium 4,4'-{ethene-1,2-diylbis[(3-sulfonato-4,1-phenylene)imino (6-chloro-1,3,5-triazine- 4,2-diyl)imino]}bis{6-[(5-carbamoyl-1-ethyl-2-hydroxy-4-methyl-6-oxo-1,6-dihydropyridin-3-yl) diazenyl]benzene-1,3-disulfonate} (CAS no. 68991 -98 -0) is considered to be not toxic to aquatic environment and cannot be classified as toxic as per the criteria mentioned in CLP regulation. 

 

Thus based on the overall studies on the toxicity of chemical on algae and invertebrates, chemical hexasodium 4,4'-{ethene-1,2-diylbis[(3-sulfonato-4,1-phenylene)imino (6-chloro-1,3,5-triazine- 4,2-diyl)imino]}bis{6-[(5-carbamoyl-1-ethyl-2-hydroxy-4-methyl-6 -oxo-1,6-dihydropyridin- 3-yl) diazenyl]benzene-1,3-disulfonate} (68991 -98 -0) was concluded as nontoxic and notclassified as per the CLP classification criteria.