Ethylidynetrimethanol

Short-term toxicity to fish

Link to relevant study record(s)

Reference

Endpoint:

short-term toxicity to fish

Type of information:

experimental study

Adequacy of study:

key study

Study period:

1983

Reliability:

2 (reliable with restrictions)

Rationale for reliability incl. deficiencies:

other: Comparable to guideline study with acceptable restrictions (no analytical monitoring)

Reason / purpose for cross-reference:

reference to same study

Qualifier:

no guideline followed

Principles of method if other than guideline:

A static acute toxicity test was performed on Alburnus alburnus in brackish water with a salinity of 7 ‰ and an exposure period of 96h. Six different concentrations were tested, which were not further specified.

GLP compliance:

no

Analytical monitoring:

no

Vehicle:

not specified

Test organisms (species):

Alburnus alburnus

Details on test organisms:

TEST ORGANISM
- Common name: bleak
- Source: wild caught in baltic sea (litoral region; brackish water)
- Length at study initiation (length definition, mean, range and SD): ca. 8 cm
- Feeding during test: no

ACCLIMATION
- Acclimation period: at least 14 days
- Acclimation conditions (same as test or not): yes (with feeding)
- Type and amount of food: commercial fish food
- Feeding frequency: daily
- Health during acclimation (any mortality observed): not reported

Test type:

static

Water media type:

brackish water

Total exposure duration:

96 h

Test temperature:

10 °C

pH:

7.8

Dissolved oxygen:

>= 5 mg/L

Salinity:

7 ‰

Nominal and measured concentrations:

at least 6 concentrations, not further specified, and one control

Details on test conditions:

TEST SYSTEM
- Test vessel:
- Material, size, headspace, fill volume: 70-L glass aquaria sealed with silicone rubber; fill volume: 60 L
- Aeration: no
- No. of organisms per vessel: 10
- No. of vessels per concentration (replicates): not reported (assumed: 1)
- No. of vessels per control (replicates): 1

TEST MEDIUM / WATER PARAMETERS
- Source/preparation of dilution water: brackish water, 40 m depth, filtered through 300 µm
- Alkalinity: 1.5 meq./L
- Culture medium different from test medium: no
- Intervals of water quality measurement: not reported

OTHER TEST CONDITIONS
- Adjustment of pH: no
- Photoperiod: 12 h dark/ 12 h light
- Light intensity: not reported


TEST CONCENTRATIONS
- Spacing factor for test concentrations: not reported (six concentrations plus control)
- Range finding study: yes

Reference substance (positive control):

no

Duration:

96 h

Dose descriptor:

LC50

Effect conc.:

> 1 000 mg/L

Nominal / measured:

nominal

Conc. based on:

not specified

Basis for effect:

mortality (fish)

Duration:

96 h

Dose descriptor:

LC50

Effect conc.:

> 10 g/L

Nominal / measured:

nominal

Basis for effect:

mortality (fish)

Sublethal observations / clinical signs:

Value reported by Linden et al. (1979): LC50 (96 h) = greater than 10,000 mg/L

Validity criteria fulfilled:

not specified

Conclusions:

The 96-h LC50 for the bleak (Alburnus alburnus) was determined to be greater than 1000 mg/L as reported by Bengtsson and > 10,000 mg/L as reported by Linden (based on nominal concentrations).

Executive summary:

The acute toxicity of trimethylolpropane to the bleak (Alburnus alburnus) was determined in a static test with brackish water (7‰, pH 7.8) and with an exposure duration of 96 h. Six different concentrations, not further specified, were tested.

The 96-h LC50 was determined to be greater than 1000 mg/L as reported by Bengtsson and > 10,000 mg/L as reported by Linden

(based on nominal concentrations).

The study satisfies the guideline requirements for an acute toxicity study with fish.

Reference: Bengtsson & Tarkpea, 1983, Linden et al., 1979

Key value for chemical safety assessment

Fresh water fish

Fresh water fish

Effect concentration:

1 000 mg/L

Additional information

Assessment is by read-across to the source substance trimethylolpropane (CAS No: 77-99-6).