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Long-term toxicity to aquatic invertebrates

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Endpoint:
long-term toxicity to aquatic invertebrates
Type of information:
other: From published data
Adequacy of study:
weight of evidence
Study period:
1985, unspecified
Reliability:
2 (reliable with restrictions)
Rationale for reliability incl. deficiencies:
other: see 'Remark'
Remarks:
Taken from publically available data published by the UK Environment Agency, and is considered accurate based on experience of the effects of the phosphates as a group. This study is read across to Phenol, isopropylated, phosphate (3:1); CAS No 68937-41-7; EC No 273-066-3. Justification is provided in the summary above.
Qualifier:
equivalent or similar to
Guideline:
other: Presumably equivalent to OECD Guideline 211 (Daphnia magna Reproduction Test)
Principles of method if other than guideline:
The test was carried out using a flow-through system using nominal exposure concentrations, with two different trade names of the substance CAS 68937-41-7 EC 273-066-3 Phenol, isopropylated, phosphate (3:1). The number of offspring in the controls was below that indicated in the current OECD test guideline (but would have been sufficient for the guideline at the time the test was performed) and there are some variations between the controls used for these tests and ones with other substances at the same time. Overall, the study is valid with restrictions.
GLP compliance:
not specified
Specific details on test material used for the study:
Details on properties of test surrogate or analogue material (migrated information):
PHYSICO-CHEMICAL PROPERTIES
- Melting point: < -20 °C
- Boiling point: > 400 °C
- Vapour pressure: 0.45 KPa at 20 °C
- Water solubility (under test conditions): 0.367 mg/L
- Henry's law constant: Not calculated
- log Pow: 4.92 — 5.17
- pKa: Not measureable
- Stability in water: Not calculated
- Stability in light: Not calculated
- pH dependance on stability: Not calculated
OTHER PROPERTIES (if relevant for this endpoint)
- Results of test for ready biodegradability: mean of 17.9 % after 28 days. Not readily biodegradable.
Analytical monitoring:
not specified
Details on sampling:
Not specified
Vehicle:
no
Details on test solutions:
Kronitex 200 - 6, 27, 72 and 154 μg/l plus control
Phosflex 31P - 0.85, 1.7, 3.5, 7.0, 14, 28 and 56 μg/l plus control
Test organisms (species):
Daphnia magna
Details on test organisms:
<24 h old. 10 per replicate, two replicates per treatment
Test type:
flow-through
Water media type:
freshwater
Limit test:
no
Total exposure duration:
21 d
Post exposure observation period:
None reported.
Hardness:
270
Test temperature:
18°C
pH:
7.2- 7.4
Dissolved oxygen:
Not reported
Salinity:
Not applicable.
Nominal and measured concentrations:
Kronitex 200 - 6, 27, 72 and 154 μg/l plus control
Phosflex 31P - 0.85, 1.7, 3.5, 7.0, 14, 28 and 56 μg/l plus control
Details on test conditions:
Not specified
Reference substance (positive control):
no
Key result
Duration:
21 d
Dose descriptor:
NOEC
Effect conc.:
0.027 mg/L
Nominal / measured:
nominal
Conc. based on:
test mat.
Remarks:
Kronitex 200
Basis for effect:
other: Survival
Remarks on result:
other: 95% survival
Key result
Duration:
21 d
Dose descriptor:
NOEC
Effect conc.:
0.006 mg/L
Nominal / measured:
nominal
Conc. based on:
test mat.
Remarks:
Kronitex 200
Basis for effect:
reproduction
Remarks on result:
other: Control response: Mean offspring/ adult = 915
Key result
Duration:
21 d
Dose descriptor:
NOEC
Effect conc.:
0.028 mg/L
Nominal / measured:
nominal
Conc. based on:
test mat.
Remarks:
Phosflex 31P
Basis for effect:
other: Survival
Remarks on result:
other: 80% survival
Key result
Duration:
21 d
Dose descriptor:
NOEC
Effect conc.:
0.028 mg/L
Nominal / measured:
nominal
Conc. based on:
test mat.
Remarks:
Phosflex 31P
Basis for effect:
reproduction
Remarks on result:
other: Control response: Mean offspring/ adult = 329
Details on results:
Sanders et al. (1985) investigated the effects of two commercial isopropylphenyl diphenyl phosphate products (Kronitex 200 and Phosflex 31P, compositions not given) on the survival and reproduction of Daphnia magna over 21 days. The test was carried out using a flow-through system using nominal exposure concentrations. The 21-day NOEC values based on survival were 0.027 mg/l for Kronitex 200 and 0.028 mg/l for Phosflex 31P (for Phosflex 31P, survival was reported to be only 45 per cent at 0.0035 mg/l compared with the control survival of 80 per cent (the survival at 0.007, 0.014 and 0.028 mg/l was similar to the control) but the significance of this finding is not commented on in the paper). The 21-day NOEC values based on reproduction were determined to be 0.006 mg/l for Kronitex 200 and 0.028 mg/l for Phosflex 31P. The experiment with Kronitex 200 used quite widely spaced concentrations (the actual concentrations tested were 0.006, 0.027, 0.072 and 0.154 mg/l) and so it is possible that the actual NOEC could be higher than indicated by this result. However, the reduction in the mean number of offspring/adult was quite severe at 0.027 mg/l (39
compared with 915 in the controls: the mean number of offspring/adults at 0.006 mg/l was 127 but this was apparently not statistically significantly different from the control population, which suggests significant variations in the numbers in the controls). There are a number of uncertainties in this study. It is not clear whether the figures presented for numbers of offspring are per adult, as stated in the paper, or are totals for each exposure, which appears more likely from the size of the values. The number of offspring in the controls was below that indicated in the current OECD test guideline (but would have been sufficient for the guideline at the time the test was performed) and there are some variations between the controls used for these tests and ones with other substances at the same time. Overall, the study is valid with restrictions.
Results with reference substance (positive control):
Not reported
Reported statistics and error estimates:
Not reported
Validity criteria fulfilled:
yes
Conclusions:
This study is read across to Phenol, isopropylated, phosphate (3:1); CAS No 68937-41-7; EC No 273-066-3. Justification is provided in the summary above.
The 21-day NOEC values based on survival were 0.027 mg/l for Kronitex 200 and 0.028 mg/l for Phosflex 31P (for Phosflex 31P, survival was reported to be only 45 per cent at 0.0035 mg/l compared with the control survival of 80 per cent (the survival at 0.007, 0.014 and 0.028 mg/l was similar to the control) but the significance of this finding is not commented on in the paper). The 21-day NOEC values based on reproduction were
determined to be 0.006 mg/l for Kronitex 200 and 0.028 mg/l for Phosflex 31P. The experiment with Kronitex 200 used quite widely spaced concentrations (the actual concentrations tested were 0.006, 0.027, 0.072 and 0.154 mg/l) and so it is possible that the actual NOEC could be higher than indicated by this result. However, the reduction in the mean number of offspring/adult was quite severe at 0.027 mg/l (39 compared with 915 in the controls: the mean number of offspring/adults at 0.006 mg/l was 127 but this was apparently not statistically significantly different from the control population, which suggests significant variations in the numbers in the controls)
Executive summary:

This study is read across to Phenol, isopropylated, phosphate (3:1); CAS No 68937-41-7; EC No 273-066-3. Justification is provided in the summary above.

The 21-day NOEC values based on survival were 0.027 mg/l for Kronitex 200 and 0.028 mg/l for Phosflex 31P (for Phosflex 31P, survival was reported to be only 45 per cent at 0.0035 mg/l compared with the control survival of 80 per cent (the survival at 0.007, 0.014 and 0.028 mg/l was similar to the control) but the significance of this finding is not commented on in the paper). The 21-day NOEC values based on reproduction were

determined to be 0.006 mg/l for Kronitex 200 and 0.028 mg/l for Phosflex 31P. The experiment with Kronitex 200 used quite widely spaced concentrations (the actual concentrations tested were 0.006, 0.027, 0.072 and 0.154 mg/l) and so it is possible that the actual NOEC could be higher than indicated by this result. However, the reduction in the mean number of offspring/adult was quite severe at 0.027 mg/l (39 compared with 915 in the controls: the mean number of offspring/adults at 0.006 mg/l was 127 but this was apparently not statistically significantly different from the control population, which suggests significant variations in the numbers in the controls)

Endpoint:
long-term toxicity to aquatic invertebrates
Type of information:
other: From published data
Adequacy of study:
weight of evidence
Study period:
1985, unspecified
Reliability:
2 (reliable with restrictions)
Rationale for reliability incl. deficiencies:
other: see 'Remark'
Remarks:
Taken from publically available data published by the UK Environment Agency, and is considered accurate based on experience of the effects of the phosphates as a group. This study is read across to Phenol, isopropylated, phosphate (3:1); CAS No 68937-41-7; EC No 273-066-3. Justification is provided in the summary above.
Qualifier:
no guideline followed
Version / remarks:
Survival and growth of amphipods (Gammarus pseudolimnaeus) over 90 days using a flow-through system
Principles of method if other than guideline:
The test was carried out using a flow-through system using nominal exposure concentrations, with two different trade names of the substance CAS 68937-41-7 EC 273-066-3 Phenol, isopropylated, phosphate (3:1). investigated the effects of the same two commercial products on the survival and growth of amphipods (Gammarus pseudolimnaeus) over 90 days using a flow-through system
GLP compliance:
not specified
Specific details on test material used for the study:
Details on properties of test surrogate or analogue material (migrated information):
PHYSICO-CHEMICAL PROPERTIES
- Melting point: < -20 °C
- Boiling point: > 400 °C
- Vapour pressure: 0.45 KPa at 20 °C
- Water solubility (under test conditions): 0.367 mg/L
- Henry's law constant: Not calculated
- log Pow: 4.92 — 5.17
- pKa: Not measureable
- Stability in water: Not calculated
- Stability in light: Not calculated
- pH dependance on stability: Not calculated
OTHER PROPERTIES (if relevant for this endpoint)
- Results of test for ready biodegradability: mean of 17.9 % after 28 days. Not readily biodegradable.
Analytical monitoring:
not specified
Details on sampling:
Not specified
Vehicle:
no
Details on test solutions:
Kronitex 200 - 0.5, 1.0, 9.0, 11, 31, 63 and 128 μg/l plus control
Phosflex 31P - 5, 10, 19, 38 and 88 μg/l plus control
Test organisms (species):
other: Gammarus pseudolimnaeus
Details on test organisms:
5-10 days old. 10 per replicate, four replicates per treatment
Test type:
flow-through
Water media type:
freshwater
Limit test:
no
Total exposure duration:
90 d
Post exposure observation period:
None reported.
Hardness:
270
Test temperature:
18°C
pH:
7.2- 7.4
Dissolved oxygen:
Not reported
Salinity:
Not applicable.
Nominal and measured concentrations:
Kronitex 200 - 0.5, 1.0, 9.0, 11, 31, 63 and 128 μg/l plus control
Phosflex 31P - 5, 10, 19, 38 and 88 μg/l plus control
Details on test conditions:
Not specified
Reference substance (positive control):
no
Key result
Duration:
90 d
Dose descriptor:
NOEC
Effect conc.:
0.011 mg/L
Nominal / measured:
nominal
Conc. based on:
test mat.
Remarks:
Kronitex 200
Basis for effect:
other: Survival
Remarks on result:
other: 75% survival
Key result
Duration:
90 d
Dose descriptor:
NOEC
Effect conc.:
0.063 mg/L
Nominal / measured:
nominal
Conc. based on:
test mat.
Remarks:
Kronitex 200
Basis for effect:
growth
Remarks on result:
other: Control response: Mean length 10.7 mm
Key result
Duration:
90 d
Dose descriptor:
NOEC
Effect conc.:
0.019 mg/L
Nominal / measured:
nominal
Conc. based on:
test mat.
Remarks:
Phosflex 31P
Basis for effect:
other: Survival
Remarks on result:
other: 90% survival
Key result
Duration:
90 d
Dose descriptor:
NOEC
Effect conc.:
>= 0.088 mg/L
Nominal / measured:
nominal
Conc. based on:
test mat.
Remarks:
Phosflex 31P
Basis for effect:
growth
Remarks on result:
other: Control response: Mean length 9.4 mm
Details on results:
Sanders et al. (1985) investigated the effects of the same two commercial products on the survival and growth of amphipods (Gammarus pseudolimnaeus) over 90 days using a flow-through system. The NOECs for survival were determined to be 0.011 μg/l for Kronitex 200 and 0.019 μg/l for Phosflex 31P (for Kronitex 200, survival was found to be statistically significantly reduced (p=0.05) at 0.031 mg/l and 0.128 mg/l but not at 0.063 mg/l, indicating a relatively poor dose-response, and the survival of the control population in this series was only 75 per cent). For growth, the mean length of the organisms at 90 days was found to be statistically significantly reduced at a concentration of 0.128 mg/l for Kronitex 200, giving a 90-day NOEC of 0.063 mg/l, but no statistically significant reductions in growth were seen at any concentration tested for Phosflex 31P (90-day NOEC above 0.088 mg/l).
Results with reference substance (positive control):
Not reported
Reported statistics and error estimates:
Not reported
Validity criteria fulfilled:
yes
Conclusions:
This study is read across to Phenol, isopropylated, phosphate (3:1); CAS No 68937-41-7; EC No 273-066-3. Justification is provided in the summary above.
Sanders et al. (1985) investigated the effects of the same two commercial products on the survival and growth of amphipods (Gammarus pseudolimnaeus) over 90 days using a flow-through system. The NOECs for survival were determined to be 0.011 μg/l for Kronitex 200 and 0.019 μg/l for Phosflex 31P (for Kronitex 200, survival was found to be statistically significantly reduced (p=0.05) at 0.031 mg/l and 0.128 mg/l but not at 0.063 mg/l, indicating a relatively poor dose-response, and the survival of the control population in this series was only 75 per cent). For growth, the mean length of the organisms at 90 days was found to be statistically significantly reduced at a concentration of 0.128 mg/l for Kronitex 200, giving a 90-day NOEC of 0.063 mg/l, but no statistically significant reductions in growth were seen at any concentration tested for Phosflex 31P (90-day NOEC above 0.088 mg/l).
Executive summary:

This study is read across to Phenol, isopropylated, phosphate (3:1); CAS No 68937-41-7; EC No 273-066-3. Justification is provided in the summary above.

Sanders et al. (1985) investigated the effects of the same two commercial products on the survival and growth of amphipods (Gammarus pseudolimnaeus) over 90 days using a flow-through system. The NOECs for survival were determined to be 0.011 μg/l for Kronitex 200 and 0.019 μg/l for Phosflex 31P (for Kronitex 200, survival was found to be statistically significantly reduced (p=0.05) at 0.031 mg/l and 0.128 mg/l but not at 0.063 mg/l, indicating a relatively poor dose-response, and the survival of the control population in this series was only 75 per cent). For growth, the mean length of the organisms at 90 days was found to be statistically significantly reduced at a concentration of 0.128 mg/l for Kronitex 200, giving a 90-day NOEC of 0.063 mg/l, but no statistically significant reductions in growth were seen at any concentration tested for Phosflex 31P (90-day NOEC above 0.088 mg/l).

Endpoint:
long-term toxicity to aquatic invertebrates
Type of information:
other: From published data
Adequacy of study:
weight of evidence
Study period:
1985, unspecified
Reliability:
2 (reliable with restrictions)
Rationale for reliability incl. deficiencies:
other: see 'Remark'
Remarks:
Taken from publically available data published by the UK Environment Agency, and is considered accurate based on experience of the effects of the phosphates as a group. This study is read across to Phenol, isopropylated, phosphate (3:1); CAS No 68937-41-7; EC No 273-066-3. Justification is provided in the summary above.
Qualifier:
no guideline available
Version / remarks:
Effects on the emergence of midges (Chironomus plumosus) over 30 days using a flow-through system.
Principles of method if other than guideline:
The test was carried out using a flow-through system using nominal exposure concentrations, with two different trade names of the substance CAS 68937-41-7 EC 273-066-3 Phenol, isopropylated, phosphate (3:1). In this test, 100 first-instar larvae were placed in a test chamber (10 × 20 × 10 cm) containing one litre of water, and 13 g of washed sand and 0.3 g of commercial dog candy were added to provide a substrate for the larvae to build a case. The organisms were fed 0.3 g of dog candy every fifth day until they had transformed into the pupal stage.
GLP compliance:
not specified
Specific details on test material used for the study:
Details on properties of test surrogate or analogue material (migrated information):
PHYSICO-CHEMICAL PROPERTIES
- Melting point: < -20 °C
- Boiling point: > 400 °C
- Vapour pressure: 0.45 KPa at 20 °C
- Water solubility (under test conditions): 0.367 mg/L
- Henry's law constant: Not calculated
- log Pow: 4.92 — 5.17
- pKa: Not measureable
- Stability in water: Not calculated
- Stability in light: Not calculated
- pH dependance on stability: Not calculated
OTHER PROPERTIES (if relevant for this endpoint)
- Results of test for ready biodegradability: mean of 17.9 % after 28 days. Not readily biodegradable.
Analytical monitoring:
not specified
Details on sampling:
Not specified
Vehicle:
no
Details on test solutions:
Kronitex 200 - 6, 27, 72 and 154 μg/l plus control
Phosflex 31P - 0.85, 1.7, 3.5, 7.0, 14, 28 and 56 μg/l plus control
Test organisms (species):
other: Chironomus plumosus
Details on test organisms:
1st instar. 100 per replicate, two replicates per treatment
Test type:
flow-through
Water media type:
freshwater
Limit test:
no
Total exposure duration:
30 d
Post exposure observation period:
None reported.
Hardness:
270
Test temperature:
18°C
pH:
7.2- 7.4
Dissolved oxygen:
Not reported
Salinity:
Not applicable.
Nominal and measured concentrations:
Kronitex 200 - 18, 36, 64, 184, 319, 718 and 1,500 μg/l plus control
Phosflex 31P - 18, 36, 64, 184, 319, 718 and 1,500 μg/l plus control
Details on test conditions:
Not specified
Reference substance (positive control):
no
Key result
Duration:
30 d
Dose descriptor:
NOEC
Effect conc.:
0.184 mg/L
Nominal / measured:
nominal
Conc. based on:
test mat.
Remarks:
Kronitex 200
Basis for effect:
other: Emergence
Remarks on result:
other: 70% emerged at day 30
Key result
Duration:
30 d
Dose descriptor:
NOEC
Effect conc.:
0.184 mg/L
Nominal / measured:
nominal
Conc. based on:
test mat.
Remarks:
Phosflex 31P
Basis for effect:
other: Emergence
Remarks on result:
other: 95% emerged at day 30
Details on results:
Sanders et al. (1985) investigated the effects of the same commercial products on the emergence of midges (Chironomus plumosus) over 30days using a flow-through system. In this test, 100 first-instar larvae were placed in a test chamber (10 × 20 × 10 cm) containing one litre of water, and 13 g of washed sand and 0.3 g of commercial dog candy were added to provide a substrate for the larvae to build a case. The organisms were fed 0.3 g of dog candy every fifth day until they had transformed into the pupal stage. The emergence of midges was less than the control at all concentrations tested, but these differences were only statistically significant (p=0.05) at concentrations of 0.319 mg/l and above for both substances. The NOECs, based on the percentage emergence after 30 days, were therefore determined as 0.184 mg/l for both products. However, the control response in the Kronitex 200 series of experiments was low (70 per cent emergence compared with 95 per cent emergence in the control for the Phosflex 31P experiments). The significance of this for the results is not clear.
Results with reference substance (positive control):
Not reported
Reported statistics and error estimates:
Not reported
Validity criteria fulfilled:
yes
Conclusions:
This study is read across to Phenol, isopropylated, phosphate (3:1); CAS No 68937-41-7; EC No 273-066-3. Justification is provided in the summary above.
Sanders et al. (1985) investigated the effects of the same commercial products on the emergence of midges (Chironomus plumosus) over 30days using a flow-through system. In this test, 100 first-instar larvae were placed in a test chamber (10 × 20 × 10 cm) containing one litre of water, and 13 g of washed sand and 0.3 g of commercial dog candy were added to provide a substrate for the larvae to build a case. The organisms were fed 0.3 g of dog candy every fifth day until they had transformed into the pupal stage. The emergence of midges was less than the control at all concentrations tested, but these differences were only statistically significant (p=0.05) at concentrations of 0.319 mg/l and above for both substances. The NOECs, based on the percentage emergence after 30 days, were therefore determined as 0.184 mg/l for both products. However, the control response in the Kronitex 200 series of experiments was low (70 per cent emergence compared with 95 per cent emergence in the control for the Phosflex 31P experiments). The significance of this for the results is not clear.
Executive summary:

This study is read across to Phenol, isopropylated, phosphate (3:1); CAS No 68937-41-7; EC No 273-066-3. Justification is provided in the summary above.

Sanders et al. (1985) investigated the effects of the same commercial products on the emergence of midges (Chironomus plumosus) over 30days using a flow-through system. In this test, 100 first-instar larvae were placed in a test chamber (10 × 20 × 10 cm) containing one litre of water, and 13 g of washed sand and 0.3 g of commercial dog candy were added to provide a substrate for the larvae to build a case. The organisms were fed 0.3 g of dog candy every fifth day until they had transformed into the pupal stage. The emergence of midges was less than the control at all concentrations tested, but these differences were only statistically significant (p=0.05) at concentrations of 0.319 mg/l and above for both substances. The NOECs, based on the percentage emergence after 30 days, were therefore determined as 0.184 mg/l for both products. However, the control response in the Kronitex 200 series of experiments was low (70 per cent emergence compared with 95 per cent emergence in the control for the Phosflex 31P experiments). The significance of this for the results is not clear.

Endpoint:
long-term toxicity to aquatic invertebrates
Type of information:
other: From published data
Adequacy of study:
weight of evidence
Study period:
1981
Reliability:
2 (reliable with restrictions)
Rationale for reliability incl. deficiencies:
other: see 'Remark'
Remarks:
Taken from publically available data published by the UK Environment Agency, and is considered accurate based on experience of the effects of the phosphates as a group. This study is read across to Tricresyl phosphate, CAS 1330-78-5 EC 215-548-8 . Justification is provided in the summary above.
Qualifier:
equivalent or similar to
Guideline:
other: Reported as OECD 202 with 21 day effects
Deviations:
not applicable
Principles of method if other than guideline:
The test was carried out on the analogous substance, Tricresyl phosphate, CAS 1330-78-5 EC 215-548-8. Adema et al. (1983) reported the results of an OECD 202 reproduction test for tricresyl phosphate (no information on purity) with Daphnia magna. Only a summary of the results is available
GLP compliance:
not specified
Specific details on test material used for the study:
Details on properties of test surrogate or analogue material (migrated information):
PHYSICO-CHEMICAL PROPERTIES
- Melting point: < -20 °C
- Boiling point: > 400 °C
- Vapour pressure: 0.28 KPa at 20 °C
- Water solubility (under test conditions): 0.271 mg/L
- Henry's law constant: Not calculated
- log Pow: 5.93
- pKa: Not measureable
- Stability in water: Not calculated
- Stability in light: Not calculated
- pH dependance on stability: Not calculated
OTHER PROPERTIES (if relevant for this endpoint)
- Results of test for ready biodegradability: mean of 24.2 % after 28 days. Not readily biodegradable.

Analytical monitoring:
not specified
Details on sampling:
Not specified
Vehicle:
no
Details on test solutions:
Not reported
Test organisms (species):
Daphnia magna
Details on test organisms:
Not reported
Test type:
not specified
Water media type:
freshwater
Limit test:
no
Total exposure duration:
21 d
Post exposure observation period:
None reported.
Hardness:
Not reported
Test temperature:
Not reported
pH:
Not reported
Dissolved oxygen:
Not reported
Salinity:
Not applicable.
Nominal and measured concentrations:
Not reported
Details on test conditions:
Not specified
Reference substance (positive control):
no
Key result
Duration:
21 d
Dose descriptor:
NOEC
Effect conc.:
0.1 mg/L
Nominal / measured:
nominal
Conc. based on:
test mat.
Basis for effect:
mortality
Key result
Duration:
21 d
Dose descriptor:
NOEC
Effect conc.:
0.1 mg/L
Nominal / measured:
nominal
Conc. based on:
test mat.
Basis for effect:
reproduction
Details on results:
Adema et al. (1983) reported the results of an OECD 202 reproduction test for tricresyl phosphate (no information on purity) with Daphnia magna. Only a summary of the results is available. As this study is reviewed by the Dutch Member State Authority, the results are considered appropriate.
Results with reference substance (positive control):
Not reported
Reported statistics and error estimates:
Not reported
Validity criteria fulfilled:
yes
Conclusions:
This study is read across to Tricresyl phosphate, CAS 1330-78-5 EC 215-548-8 . Justification is provided in the summary above.
Adema et al. (1983) reported the results of an OECD 202 reproduction test for tricresyl phosphate (no information on purity) with Daphnia magna. Only a summary of the results is available. As this study is reviewed by the Dutch Member State Authority, the results are considered appropriate.
Executive summary:

This study is read across to Tricresyl phosphate, CAS 1330-78-5 EC 215-548-8 . Justification is provided in the summary above.

Adema et al. (1983) reported the results of an OECD 202 reproduction test for tricresyl phosphate (no information on purity) with Daphnia magna. Only a summary of the results is available. As this study is reviewed by the Dutch Member State Authority, the results are considered appropriate.

Description of key information

A weight of evidence approach is applied.

Key value for chemical safety assessment

EC10, LC10 or NOEC for freshwater invertebrates:
0.033 mg/L

Additional information

A weight of evidence approach, utilising read across to structural analogues is undertaken. As a weight of evidence approach, it is considered appropriate to utilise a geometric mean for the data on Daphnia in order to derive an appropriate NOEC for use in hazard assessment. The document “Guidance on information requirements and chemical safety assessment Chapter R.10: Characterisation of dose [concentration]-response for environment”states under section“R.10.2.2 Evaluation and interpretation of data” that:

 

Where there is more than one set of data on the same species, (strain if known), endpoint, duration, life stage and testing condition the greatest weight is attached to the most reliable and relevant one. When there is more than one set of data with the same reliability rating, it might be necessary tolook into more detail at the study reports to see whether a specific reason could explain the difference. If no explanation can be found and the results are for the same species and endpoints and are not more than one order of magnitude apart, they can be harmonised by a geometric mean.

 

A geometric mean 21-day NOEC is therefore applied for the purposes of hazard assessment.

Full justification for this approach is attached below under the "documents" section.