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EC number: 945-518-6
CAS number: -
ErC50 72h >100 mg/LThe major effect on algal growth was related to the dyeing properties of the test item, which overruled any intrinsic toxic effect.
The test was performed on a similar
substance (read-across from supporting substance-structural analogue or
The study procedures were based on the EU
C.3, the OECD guideline 201 and ISO Standard 8692.
Range-finding test: Algal suspensions were
exposed to concentrations of 0.1, 1.0, 10 and 100 mg/L.
An additional range-finding test was
performed if the substance would indirectly inhibit the growth of green
algae owing to the colour of the test solutions.
Tests were performed including both direct
and indirect exposure in order to confirm the results of the
Main test: concentrations tested were 1.0,
2.2, 4.6, 10, 22, 46 and 100 mg/l. The colour of the test substance
treated solutions ranged from yellow to dark brown. All were clear
The actual exposure concentrations
remained above 80% relative to the initial concentrations at
concentrations. in both tests, a concentration dependent increase was
observed in growth rate reduction and cell growth inhibition.
Algal growth was almost completely reduced
at the highest concentration of 100 mg/L after indirect exposure.
70% of the reduction of growth rate was related to absorption of
wavelengths by the colour
of the test solutions at the concentrations up to and including 100
mg/L, the maximum concentration
to be tested. Hence, the actual ErC50 exceeded 100 mg/L.
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