sodium 5-(4-amino-6-(4-((E)-4-(4-(bis(2-hydroxyethyl)amino)-6-(8-hydroxy-7-((E)-phenyldiazenyl)-3,6-disulfonatonaphthalen-1-ylamino)-1,3,5-triazin-2-ylamino)-2-sulfonatostyryl)-3-sulfonatophenylamino)-1,3,5-triazin-2-ylamino)-4-hydroxy-3-((E)-phenyldiazenyl)naphthalene-2,7-disulfonate

Short-term toxicity to aquatic invertebrates

Description of key information

EC50 (48h) > 100 mg/l

Key value for chemical safety assessment

Fresh water invertebrates

Fresh water invertebrates

Effect concentration:

100 mg/L

Additional information

No information on the "short-term toxicity to aquatic invertebrates" of the Target Substance is available. However data on a Similar Substance has been taken into account for the assessment. More details on the substance similarity are reported in section 13.

In the acute immobilisation test with Daphnia magna (STRAUS), the effects of the limit concentration of 114 mg/L test item were determined under static conditions over a period of 48 hours according to OECD 202 (2004), equivalent to the Council Regulation (EC) No.440/2008 Method C.2 (2008). A stock solution of 114 mg/L of the test item was freshly prepared with dilution water before the start of the exposure (at 0 hours), homogenised by manual agitation and used for testing. Corrected for purity, the limit concentration was 100 mg/L of pure (anhydrous) substance. The test item solution was red coloured and visually clear throughout the exposure period. Twenty daphnids were exposed to the limit concentration and the control. The concentrations of the test item were analytically verified via spectrophotometric analysis at the start (0 hours) and at the end of the exposure (48 hours) in the limit concentration and the control. The measured concentration in the limit concentration of the test item at the start of the exposure (0 hours) was 107 mg/L and 106 mg/L at the end of the exposure (48 hours). The measured test item concentrations were within ± 20 % of the nominal concentration. This indicates that the limit test item concentration was successfully maintained for the duration of the test. Therefore, the effect levels were based on the nominal concentration of the test item, corrected for purity. The water quality parameters (i.e. pH-value and dissolved oxygen concentration), measured at the start (0 hours) and at the end of the exposure (48 hours), were within the acceptable limits. The validity criteria of the test guidelines were fulfilled. At the limit concentration of 114 mg/L of the test item, which is equivalent to 100 mg/L pure (anhydrous) substance, no effects were observed on Daphnia magna. In conclusion, the following endpoint values were determined on the basis of the nominal test item concentrations, corrected for purity after 24 h and 48 h of exposure: EC10-, EC50- and EC100-value is greater than 100 mg/L.