Registration Dossier

Administrative data

Endpoint:
acute toxicity: oral
Type of information:
experimental study
Adequacy of study:
key study
Study period:
1964
Reliability:
2 (reliable with restrictions)
Rationale for reliability incl. deficiencies:
guideline study

Data source

Reference
Reference Type:
publication
Title:
Unnamed
Year:
1964

Materials and methods

Test guideline
Qualifier:
no guideline available
Principles of method if other than guideline:
Acute oral study. LD50 values were computed by the method of Litchfield & Wilcoxon (1949).
GLP compliance:
no
Test type:
standard acute method
Limit test:
no

Test material

Reference
Name:
Unnamed
Type:
Constituent
Test material form:
liquid

Test animals

Species:
rat
Strain:
Osborne-Mendel
Sex:
male/female

Administration / exposure

Route of administration:
oral: gavage
Vehicle:
unchanged (no vehicle)
Details on oral exposure:
All doses given by intubation.
Doses:
1000 and 2000
No. of animals per sex per dose:
10 mice per dose.
Control animals:
no
Details on study design:
Groups of 10 rats were evenly divided by sex and fasted for approximately 18 hours prior to treatment.
Animals had access to water at all times and food was replaced in cages as soon as animals received their respective doses.

Results and discussion

Effect levels
Key result
Sex:
male/female
Dose descriptor:
LD50
Effect level:
1 390 mg/kg bw
Based on:
test mat.
Mortality:
None indicated at 1000.
Deaths occured at 2000 mg/kg.
Clinical signs:
Depression within 1hr after clinical treatment, scrawny appearance, porphyrin-like deposit around the eyes and nose.
Body weight:
Not recorded
Gross pathology:
Gross pathology showed discoloured liver, irritated gastro-intestinal tract, stomach haemorrhage, yellowish material attached to the intestines.

Applicant's summary and conclusion

Interpretation of results:
Category 4 based on GHS criteria
Conclusions:
The LD50 is 1390 mg/kg.
Executive summary:

The LD50 of Cuminic Aldehyde was determined to be 1390 mg/kg.