Benzonitrile, 4-[(1,6-dihydro-4-hydroxy-6-oxo-2-pyrimidinyl)amino]-

Adsorption / desorption

Link to relevant study record(s)

Reference

Endpoint:

adsorption / desorption: screening

Type of information:

experimental study

Adequacy of study:

key study

Study period:

2015-10-03 to 2015-10-03

Reliability:

1 (reliable without restriction)

Rationale for reliability incl. deficiencies:

guideline study

Qualifier:

according to guideline

Guideline:

OECD Guideline 121 (Estimation of the Adsorption Coefficient (Koc) on Soil and on Sewage Sludge using High Performance Liquid Chromatography (HPLC))

Deviations:

no

Qualifier:

according to guideline

Guideline:

EU Method C.19 (Estimation of the Adsorption Coefficient (KOC) on Soil and Sewage Sludge Using High Performance Liquid Chromatography (HPLC))

Deviations:

no

GLP compliance:

yes (incl. QA statement)

Type of method:

HPLC estimation method

Media:

soil

Specific details on test material used for the study:

SOURCE OF TEST MATERIAL
- Source and lot/batch No.of test material: I15CB0987
- Expiration date of the lot/batch: 2015-12-05 (retest date)
- Purity test date: 2015-08-17

STABILITY AND STORAGE CONDITIONS OF TEST MATERIAL
- Storage condition of test material: at room temperature
- Stability under test conditions: no data

Radiolabelling:

no

Test temperature:

no data

Details on study design: HPLC method:

EQUIPMENT
- Apparatus: Acquity UPLC system (Waters, Milford, MA, USA), Acquity UPLC TUV detector (Waters).
- Type: UPLC.
- Type, material and dimension of analytical (guard) column: Acquity UPLC HSS Cyano, 100 mm x 2.1 mm i.d., dp = 1.8 µm (Waters).
- Detection: 210 nm

MOBILE PHASES
- Type: 30/70 (v/v) methanol/water
- Solutes for dissolving test and reference substances: methanol

REFERENCE SUBSTANCES
- Identity: combined reference solution containing acetanilide, monuron, 2,5-Dichloroaniline, napthalène, benzoic acid phenylester, Fenthion

DETERMINATION OF RETENTION TIMES
- Quantity of test substance introduced in the column: 10 mg/L.
- Intervals of calibration: no data

REPETITIONS
- Number of determinations: 2 times (test item solution), 1 time (blank solution)

EVALUATION
- Calculation of capacity factors k': from retention time of reference items, the test item, and the non-sorbed chemical item
- Determination of the log Koc value: a correlation of log k' versus log Koc of the reference items is plotted using linear regression - the log k' value of the test item is then used to calculate its log Koc value

Key result

Type:

log Koc

Value:

2.21 dimensionless

Remarks on result:

other: mean of 2 analyses

Details on results (HPLC method):

- Retention times of reference substances used for calibration:
* Formamide: 0.695 - 0.693 min.
* Acetanilide: 1.330 - 1.331 min.
* Monuron: 1.560 - 1.561 min.
* 2,5-Dichloroaniline: 3.130 - 3.129 min.
* Naftalene: 3.838 - 3.838 min.
* Benzoic acid phenylester: 6.076 - 6.072 min.
* Fenthion: 13.042 - 12.980 min.
- Details of fitted regression line (log k' vs. log Koc): slope = 0.636, intercept = -0.996, coefficient of regression = 0.96
- Average retention data for test substance: 2.493 min
- Koc of test substance = 160

Validity criteria fulfilled:

yes

Conclusions:

The mean log Koc of T002326 was determined to be 2.21 using the HPLC estimation method according to OECD guideline 121. These results indicate that T002326 will likely not be adsorbed by organic carbon in soil. The results of the test can be considered reliable without restriction.

Description of key information

This endpoint is covered with a GLP-study performed according to standard test guideline OECD 121. The log Koc of the test substance was determined to be 2.21 using the HPLC estimation method. Based on the relatively low logKoc no significant adsorption to soil or sediment is expected.

Key value for chemical safety assessment

Koc at 20 °C:

160

Additional information

[LogKoc: 2.21]