2-chloro-4-nitrophenol

Acute Toxicity: dermal

Administrative data

Endpoint:

acute toxicity: dermal

Type of information:

experimental study

Adequacy of study:

weight of evidence

Reliability:

4 (not assignable)

Rationale for reliability incl. deficiencies:

secondary literature

Justification for type of information:

Data is from secondary source

Data source

Reference

Reference Type:

secondary source

Title:

INITIAL SUBMISSION: TOXICOLOGICAL INVESTIGATION OF 2-CHLOR0-4-NITROPHENOL WITH COVER LETIER DATED 081392

Author:

National Technical Reports Library

Year:

1992

Bibliographic source:

INITIAL SUBMISSION: TOXICOLOGICAL INVESTIGATION OF 2-CHLOR0-4-NITROPHENOL, OTS0570573, 1992

Materials and methods

Test guideline

Qualifier:

according to guideline

Guideline:

other: as metioned below

Principles of method if other than guideline:

Acute Dermal toxicity of 2-chloro-4-nitrophenol (619-08-9) in rabbits.

GLP compliance:

not specified

Test type:

other: not specified

Limit test:

no

Test material

Test material information

Constituent 1

Reference substance name:

2-chloro-4-nitrophenol

EC Number:

210-578-8

EC Name:

2-chloro-4-nitrophenol

Cas Number:

619-08-9

Molecular formula:

C6H4ClNO3

IUPAC Name:

2-chloro-4-nitrophenol

Details on test material:

- IUPAC Name: 2-chloro-4-nitrophenol
- Common Name: Nitrofungin
- Mol. formula: C6H4ClNO3
- Molecular Weight: 173.555 g/mol
- Smiles: c1(cc(c(O)cc1)Cl)[N+](=O)[O-]
- InChI: 1S/C6H4ClNO3/c7-5-3-4(8(10)11)1-2-6(5)9/h1-3,9H

Specific details on test material used for the study:

- IUPAC Name: 2-chloro-4-nitrophenol
- Common Name: Nitrofungin
- Mol. formula: C6H4ClNO3
- Molecular Weight: 173.555 g/mol
- Smiles: c1(cc(c(O)cc1)Cl)[N+](=O)[O-]
- InChI: 1S/C6H4ClNO3/c7-5-3-4(8(10)11)1-2-6(5)9/h1-3,9H

Test animals

Species:

rabbit

Strain:

New Zealand White

Sex:

male/female

Details on test animals or test system and environmental conditions:

not specified

Administration / exposure

Type of coverage:

occlusive

Vehicle:

corn oil

Details on dermal exposure:

- Concentration in vehicle: 50%

Duration of exposure:

not specified

Doses:

750, 1000, 1500, 2000, 2250, 3000 and 4000 mg/kg

No. of animals per sex per dose:

Total = 7 (Male/Female)
750 mg/ kg – 1 Male
1000 mg/ kg – 1 Female
1500 mg/ kg – 1 Female
2000 mg/ kg – 1 Male
2250 mg/ kg – 1 Male
3000 mg/ kg – 1 Male
4000 mg/ kg – 1 Male

Control animals:

not specified

Details on study design:

- Duration of observation period following administration: No data
- Frequency of observations and weighing: Weighing was performed.
- Necropsy of survivors performed: yes
- Other examinations performed: clinical signs, body weight, organ weights, histopathology, examinations were observed.

Statistics:

not specified

Results and discussion

Preliminary study:

not specified

Effect levels

Sex:

male/female

Dose descriptor:

LD50

Effect level:

1 500 - 2 000 mg/kg bw

Based on:

test mat.

Remarks on result:

other: 50% mortality was observed.

Mortality:

50% mortality was observed at the range of 1500 – 2000 mg/kg bw

Clinical signs:

other: Animals were experienced lethargy after few hours of dosing. The survivors were inactive and had a poor appetite.

Gross pathology:

At autopsy, visceral changes observed macroscopically were confined to tissue discoloration underneath the area of treatment.

Other findings:

not specified

Applicant's summary and conclusion

Interpretation of results:

Category 4 based on GHS criteria

Conclusions:

The LD50 was considered in the Range of 1500 – 2000 mg/kg bw, when New Zealand White rabbits were treated occlusively with 2-chloro-4-nitrophenol (619-08-9) by dermal application.

Executive summary:

The acute dermal toxicity of 2-chloro-4-nitrophenol was tested in 7 New Zealand WhiteMale and Female rabbits by applying the test substance to the closely clipped, intact skin of rabbits and the treated areas covered with plastic shield to prevent loss of the sample. The dose concentrations used were 750, 1000, 1500, 2000, 2250, 3000 and 4000 mg/kg in 50% suspension in corn oil (Vehicle). Rabbits were observed for clinical signs, body weight, organ weights, and histopathology, examinations. 50% mortality was observed in treated rabbits. During the observation animals were survived from 6 hours to overnight period. Animals were experienced lethargy after few hours of dosing. The survivors were inactive and had a poor appetite. Body weight was noted in Table for all the animals. At autopsy, visceral changes observed macroscopically were confined to tissue discoloration underneath the area of treatment. Therefore, LD50 was considered in the Range of 1500 – 2000 mg/kg bw, when New Zealand White rabbits were treated occlusively with 2-chloro-4-nitrophenol (619-08-9) by dermal application.