2-chloro-4-nitrophenol

Acute Toxicity: oral

Administrative data

Endpoint:

acute toxicity: oral

Type of information:

experimental study

Adequacy of study:

weight of evidence

Reliability:

2 (reliable with restrictions)

Rationale for reliability incl. deficiencies:

data from handbook or collection of data

Justification for type of information:

Data is from Handbook

Data source

Reference

Reference 1

Reference Type:

review article or handbook

Title:

Toxicology of Drugs and Chemicals

Author:

William B. Deichmann and Horace W. Gerarde

Year:

1969

Bibliographic source:

Toxicology of Drugs and Chemicals, P. 169, 1969

Reference 2

Reference Type:

review article or handbook

Title:

Acute oral toxicity of 2-chloro-4-nitrophenol (619-08-9)

Author:

Richard J. Lewis, Sr.

Year:

2012

Bibliographic source:

Sax's Dangerous Properties of Industrial Materials. 12th Edition, 2012

Reference 3

Reference Type:

secondary source

Title:

INITIAL SUBMISSION: TOXICOLOGICAL INVESTIGATION OF 2-CHLOR0-4-NITROPHENOL WITH COVER LETIER DATED 081392

Author:

National Technical Reports Library

Year:

1992

Bibliographic source:

INITIAL SUBMISSION: TOXICOLOGICAL INVESTIGATION OF 2-CHLOR0-4-NITROPHENOL, OTS0570573, 1992

Materials and methods

Test guideline

Qualifier:

according to guideline

Guideline:

other: as metioned below

Principles of method if other than guideline:

Acute oral toxicity of 2-chloro-4-nitrophenol (619-08-9) in albino rats.

GLP compliance:

not specified

Test type:

other: not specified

Limit test:

no

Test material

Test material information

Constituent 1

Reference substance name:

2-chloro-4-nitrophenol

EC Number:

210-578-8

EC Name:

2-chloro-4-nitrophenol

Cas Number:

619-08-9

Molecular formula:

C6H4ClNO3

IUPAC Name:

2-chloro-4-nitrophenol

Details on test material:

- IUPAC Name: 2-chloro-4-nitrophenol
- Common Name: Nitrofungin
- Mol. formula: C6H4ClNO3
- Molecular Weight: 173.555 g/mol
- Smiles: c1(cc(c(O)cc1)Cl)[N+](=O)[O-]
- InChI: 1S/C6H4ClNO3/c7-5-3-4(8(10)11)1-2-6(5)9/h1-3,9H

Specific details on test material used for the study:

- IUPAC Name: 2-chloro-4-nitrophenol
- Common Name: Nitrofungin
- Mol. formula: C6H4ClNO3
- Molecular Weight: 173.555 g/mol
- Smiles: c1(cc(c(O)cc1)Cl)[N+](=O)[O-]
- InChI: 1S/C6H4ClNO3/c7-5-3-4(8(10)11)1-2-6(5)9/h1-3,9H

Test animals

Species:

rat

Strain:

Sprague-Dawley

Sex:

male/female

Details on test animals or test system and environmental conditions:

- Diet (e.g. ad libitum): Diet was provided to the rats.

Administration / exposure

Route of administration:

other: Stomach tube

Vehicle:

corn oil

Remarks:

50%

Details on oral exposure:

- Concentration in vehicle: 700, 800, 900, and 1000 mg/kg

Doses:

700, 800, 900, and 1000 mg/kg

No. of animals per sex per dose:

Total = 23 (Male/Female)
700 mg/ kg – 3 Males and 2 Females
800 mg/ kg – 3 Males and 2 Females
900 mg/ kg – 4 Males and 4 Females
1000 mg/ kg – 2 Males and 3 Females

Control animals:

not specified

Details on study design:

- Duration of observation period following administration: No data
- Frequency of observations and weighing: Weighing was performed.
- Necropsy of survivors performed: yes
- Other examinations performed: clinical signs, body weight, organ weights, histopathology, examinations were observed.

Statistics:

not specified

Results and discussion

Preliminary study:

not specified

Effect levels

Sex:

male/female

Dose descriptor:

LD50

Effect level:

900 mg/kg bw

Based on:

test mat.

Remarks on result:

other: 50% mortality was observed.

Mortality:

50% mortality was observed at 900 mg/kg bw

Clinical signs:

other: Excessive salivation and moderate diarrhea was observed in animals those are survived overnight period after dosing.

Gross pathology:

Liver damage was observed after macroscopic examination.

Other findings:

not specified

Any other information on results incl. tables

Table – The Acute Oral Toxicity (LD50) of 2-chloro-4-nitrophenol for rats.

Animal No. - Sex	Weight Gm	Dose Mg/kg	Fate
1 – Male	240	700	Survived
2 - Female	200	700	Survived
3 - Female	195	700	Died
4 – Male	225	700	Died
5 – Male	250	700	Survived
6 - Male	215	800	Died
7 - Male	230	800	Survived
8 - Male	245	800	Survived
9 - Female	200	800	Died
10 - Female	210	800	Survived
11 - Female	220	900	Died
12 – Male	248	900	Survived
13 - Male	220	900	Survived
14 - Female	210	900	Died
15 - Female	190	900	Died
16 - Female	208	900	Survived
17 - Male	230	900	Survived
18 - Male	260	900	Died
19 - Male	245	1000	Died
20 - Female	200	1000	Died
21 - Female	200	1000	Died
22 - Female	225	1000	Died
23 - Male	240	1000	Died

 

Applicant's summary and conclusion

Interpretation of results:

Category 4 based on GHS criteria

Conclusions:

The LD50 was considered to be 900 mg/kg bw, when Sprague dawley rats were treated with 2-chloro-4-nitrophenol (619-08-9) orally.

Executive summary:

The acute oral toxicity of 2-chloro-4-nitrophenol was tested in 23 Sprague dawley Male and Female rats by administering the test substance through stomach tube. The dose concentrations used were 700, 800, 900 and 100mg/kg in 50% suspension in corn oil (Vehicle). Diet was provided to the rats. Rats were observed for clinical signs, body weight, organ weights, and histopathology, examinations. 50% mortality was observed in treated rats. During the observation period of several days, Excessive salivation and moderate diarrhea was observed in animals those are survived overnight period after dosing. Body weight was noted in Table for all the animals. Liver damage was observed after macroscopic examination. Therefore, LD50 was considered to be 900 mg/kg bw, when Sprague dawley rats were treated with 2-chloro-4-nitrophenol (619-08-9) orally.