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Diss Factsheets

Ecotoxicological information

Toxicity to aquatic algae and cyanobacteria

Administrative data

Endpoint:
toxicity to aquatic algae and cyanobacteria
Type of information:
experimental study
Adequacy of study:
key study
Reliability:
1 (reliable without restriction)
Rationale for reliability incl. deficiencies:
comparable to guideline study

Data source

Reference
Reference Type:
study report
Title:
Unnamed
Year:
2016
Report date:
2016

Materials and methods

Test guideline
Qualifier:
according to guideline
Guideline:
OECD Guideline 201 (Alga, Growth Inhibition Test)
Deviations:
no
GLP compliance:
yes (incl. QA statement)

Test material

1
Chemical structure
Reference substance name:
Naloxone
EC Number:
207-365-7
EC Name:
Naloxone
Cas Number:
465-65-6
Molecular formula:
C19H21NO4
IUPAC Name:
(1S,5R,13R,17S)-10,17-dihydroxy-4-(prop-2-en-1-yl)-12-oxa-4-azapentacyclo[9.6.1.0^{1,13}.0^{5,17}.0^{7,18}]octadeca-7(18),8,10-trien-14-one

Sampling and analysis

Analytical monitoring:
yes
Remarks:
HPLC-UV

Test organisms

Test organisms (species):
Pseudokirchneriella subcapitata (previous names: Raphidocelis subcapitata, Selenastrum capricornutum)
Details on test organisms:
Recently renamed as Raphidocelis subcapitata (KORSHIKOV). The algae were supplied by the „Sammlung von Algenkulturen, Albrecht-von-Haller-Institut für Pflanzenwissenschaften, Universität Göttingen", 37073 Göttingen, Germany.

Endpoints: Yield and growth rate of the algae

Study design

Test type:
static
Limit test:
no
Total exposure duration:
72 h
Post exposure observation period:
A microscopic examination of the shape of the algal cells was done after 72 hours.
The cell density on each observation time was determined by spectrophotometric measurement. A stat
istical analysis was then conducted.

Test conditions

Test temperature:
21 to 22°C
pH:
8 to 9
Nominal and measured concentrations:
A filtrate of nominal 100 mg test item/L and dilutions of 1:3, 1:9, 1:27 and 1:81 of this filtrate and a control corresponding to following mean measured concentrations of the test item:
72.5, 25.0, 7.78, 2.67 and 0.891 mg test item/L and a control.
Reference substance (positive control):
yes
Remarks:
Potassium dichromate

Results and discussion

Effect concentrationsopen allclose all
Duration:
72 h
Dose descriptor:
EC50
Effect conc.:
18.8 mg/L
Nominal / measured:
meas. (geom. mean)
Basis for effect:
other: Yield
Key result
Duration:
72 h
Dose descriptor:
EC50
Effect conc.:
51.2 mg/L
Nominal / measured:
meas. (geom. mean)
Basis for effect:
growth rate
Key result
Duration:
72 h
Dose descriptor:
NOEC
Effect conc.:
2.67 mg/L
Nominal / measured:
meas. (geom. mean)
Basis for effect:
growth rate
Key result
Duration:
72 h
Dose descriptor:
LOEC
Effect conc.:
7.78 mg/L
Nominal / measured:
meas. (geom. mean)
Basis for effect:
growth rate
Duration:
72 h
Dose descriptor:
NOEC
Effect conc.:
2.67 mg/L
Nominal / measured:
meas. (geom. mean)
Basis for effect:
other: Yield
Duration:
72 h
Dose descriptor:
LOEC
Effect conc.:
7.78 mg/L
Nominal / measured:
meas. (geom. mean)
Basis for effect:
other: Yield
Details on results:
The cell density on each observation time was determined by spectrophotometric measurement. Therefore, defined volumes of the algal suspensions from all replicates and from the blanks were sampled after 24, 48 and 72 hours of exposure, and were not replaced. The algal cell densities were calculated by subtracting the absorption of the blanks, from each of the measured absorption of the test media (with algae). Based on the counted cell densities and the absorption from an algal suspension and its dilutions, a linear regression was performed for the calculation of the cell densities of the replicates during the test.

Applicant's summary and conclusion

Validity criteria fulfilled:
yes
Conclusions:
The 72-hour EyC50 was calculated to be 18.8 mg test item/L and the 72-hour ErC50 value was calculated to be 51.2 mg test item/L. The 72-hour NOEyC was determined to be 2.67 mg test item/L and the associated 72-hour LOEyC was 7.78 mg test item/L. The 72-hour NOErC was determined to be 2.67 mg test item/L and the associated 72-hour LOErC was 7.78 mg test item/L.