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EC number: 221-831-7 | CAS number: 3248-91-7
Endpoint summary
Administrative data
Description of key information
Short term toxicity to aquatic invetebrate:
Short term toxicity of test chemical 4-[(4-amino-3-methylphenyl)(4-imino-3-methylcyclohexa-2,5-dien-1-ylidene)methyl]-2-methylaniline hydrochloride (CASno. 3248-91-7) was estimated by using OECD QSAR tool box v3.3 with log Kow as primary descriptor and considering the six closest read across substances. When test chemical was exposed for 48 hrs on Daphnia magna EC50 value was estimated to be 508.66 mg/L. Since EC50 value is greater than 100 mg/L it is concluded that this test chemical can be considered to be not classified according to CLP classification criteria.
Toxicity to aquatic algae and cyanobacteria:
Toxicity of test chemical 4-[(4-amino-3-methylphenyl)(4-imino-3-methylcyclohexa-2,5-dien-1-ylidene)methyl]-2-methylaniline hydrochloride (CASno. 3248-91-7) was estimated by using OECD QSAR tool box v3.3 with log Kow as primary descriptor and considering the seven closest read across substances. When test chemical was exposed for 72 hrs on Pseudokirchneriella subcapitata (previous names: Raphidocelis subcapitata, Selenastrum capricornutum) alga, EC50 value was estimated to be 353.60 mg/L. Since EC50 value is greater than 100 mg/L it is concluded that this test chemical can be considered to be not classified according to CLP classification criteria.
Additional information
Short term toxicity to aquatic invetebrate:
Two different predicted data studies have been conducted for target chemical
4-[(4-amino-3-methylphenyl)(4-imino-3-methylcyclohexa-2,5-dien-1-ylidene)methyl]-2-methylaniline hydrochloride (CASno. 3248-91-7) and one experimental study conducted for its read across chemical to determine short term toxicity to aquatic invetebrate endpoint and their results are summarized below.
In first weight of evidence predicted data study the short term toxicity of test chemical 4-[(4-amino-3-methylphenyl)(4-imino-3-methylcyclohexa-2,5-dien-1-ylidene)methyl]-2-methylaniline hydrochloride (CASno. 3248-91-7) was estimated by using OECD QSAR tool box v3.3 with log Kow as primary descriptor and considering the six closest read across substances. When test chemical was exposed for 48 hrs on Daphnia magna EC50 value was estimated to be 508.66 mg/L. Since EC50 value is greater than 100 mg/L it is concluded that this test chemical can be considered to be not classified according to CLP classification criteria.
Another weight of evidence predicted data study done by EPI suite, ECOSAR version 1.1, on the basis of similarity of structure to chemicals for which the aquatic toxicity has been previously measured by structure-activity relationships (SARs) program, the LC 50 value for short term toxicity to aquatic invertebrates was predicted.
On the basis of this program, the LC 50 value for short term toxicity to aquatic invertebrates was predicted to be 385.045 mg/l for test chemical4-[(4-amino-3-methylphenyl)(4-imino-3-methylcyclohexa-2,5-dien-1-ylidene)methyl]-2-methylaniline hydrochloride (CAS no. 3248-91-7)in 48 hrs.
Based on this value it can be concluded that the substance is considered to be not toxic to aquatic environment and can not be classified in category as per the criteria mentioned in CLP regulation.
Last study was done from authoritative database ( Ecotox. 2017) in this study short term toxicity toLitopenaeus schmitti(Southern white shrimp) was determined for 48 hrs.The study was based on the effects of the read across compound 4 -[(4 -dimethylamino phenyl)-phenylmethylidene]cyclohexa-2,5-dien-1-ylidene]-dimethylazanium chloride (CAS no. 569-64-2) onLitopenaeus schmittiin a static salt water system.Litopenaeus schmitti(Southern white shrimp) was used as a test organism. Based on effect on mortality of the test organismLitopenaeus schmitti, the 48 hr LC50 value was determined to be 77170 mg/L (LC50 value ranges from 60470 to 109120 mg/l).Thus, based on the LC50 value, it can be concluded that the substance 4 -[(4 -dimethylaminophenyl)-phenylmethyli dene]cyclohexa-2,5-dien-1-ylidene]-dimethylazanium chloride can be considered as non-toxic to aquatic organisms and thus can be considered to be not classified as per the CLP classification criteria.
Based on the results of all the studies from different sources mentioned above for target chemical
4-[(4-amino-3-methylphenyl)(4-imino-3-methylcyclohexa-2,5-dien-1-ylidene)methyl]-2-methylaniline hydrochloride (CASno. 3248-91-7) and its read across chemical EC50 value falls in range of 385.045 - 508.66 mg/L and by this range value it is concluded that that test chemical can be considered as not classified according to CLP classification criteria.
Toxicity to aquatic algae and cyanobacteria:
Predicted data studies have been conducted for test chemical
4-[(4-amino-3-methylphenyl)(4-imino-3-methylcyclohexa-2,5-dien-1-ylidene)methyl]-2-methylaniline hydrochloride (CASno. 3248-91-7) and their results are summarized below for toxicity to aquatic algae and cyanobacteria endpoint.
In the first study toxicity of test chemical 4-[(4-amino-3-methylphenyl)(4-imino-3-methylcyclohexa-2,5-dien-1-ylidene)methyl]-2-methylaniline hydrochloride (CASno. 3248-91-7) was estimated by using OECD QSAR tool box v3.3 with log Kow as primary descriptor and considering the seven closest read across substances. When test chemical was exposed for 72 hrs on Pseudokirchneriella subcapitata (previous names: Raphidocelis subcapitata, Selenastrum capricornutum) alga, EC50 value was estimated to be 353.60 mg/L. Since EC50 value is greater than 100 mg/L it is concluded that this test chemical can be considered to be not classified according to CLP classification criteria.
In another study the short-term toxicity of the test substance 4-[(4-amino-3-methylphenyl)(4-imino-3-methylcyclohexa-2,5-dien-1-ylidene)methyl]-2-methylaniline hydrochloride (CAS no. 3248-91-7) to green algae was predicted using EPI Suite ECOSAR version 1.10. On the basis of effects observed in a static freshwater system during a 96 hr exposure, the effect concentration (EC50) for the substance is estimated to be 240.314 mg/L. Based on this value, it can be concluded that the test chemical 4-[(4-amino-3-methylphenyl)(4-imino-3-methylcyclohexa-2,5-dien-1-ylidene)methyl]-2-methylaniline hydrochloride can be considered as non-toxic to green algae at environmentally relevant concentrations and can be considered not-classified as per the CLP classification criteria.
On the basis of both studies mentioned above EC 50 value for test chemical was in range of 240.314 - 353.60 mg/Land therefore it is concluded that this test chemical can be considered to be not classified according to CLP classification criteria.
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