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Toxicity to aquatic algae and cyanobacteria

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Reference
Endpoint:
toxicity to aquatic algae and cyanobacteria
Type of information:
experimental study
Adequacy of study:
key study
Reliability:
2 (reliable with restrictions)
Rationale for reliability incl. deficiencies:
data from handbook or collection of data
Justification for type of information:
Data is from peer reveiwed journal.
Qualifier:
according to
Guideline:
ISO 10253 (Water quality - Marine Algal Growth Inhibition Test with Skeletonema costatum and Phaeodactylum tricornutum)
Version / remarks:
No data available
Deviations:
not specified
Principles of method if other than guideline:
Short term toxicity study of N-Methyl-1,3-propanediamine on the growth of marine algae Skeletonema costatum for 72 hrs.
GLP compliance:
not specified
Specific details on test material used for the study:
- Name of test material: (3-aminopropyl)(methyl)amine
- Molecular formula: C4H12N2
- Molecular weight: 88.1528 g/mol
- Smiles notation: N(CCCN)C
- InChl: 1S/C4H12N2/c1-6-4-2-3-5/h6H,2-5H2,1H3
- Substance type: Organic
- Physical state: Liquid
Analytical monitoring:
not specified
Details on sampling:
No data available
Vehicle:
not specified
Details on test solutions:
No data available
Test organisms (species):
Skeletonema costatum
Details on test organisms:
No data available
Test type:
static
Water media type:
other: Marine water
Total exposure duration:
72 h
Remarks on exposure duration:
No data available
Post exposure observation period:
No data available
Hardness:
No data available
Test temperature:
20 ± 2◦ C
pH:
No data available
Dissolved oxygen:
No data available
Salinity:
No data available
Conductivity:
No data available
Nominal and measured concentrations:
10-10,000 mg/l
Details on test conditions:
- No. of organisms per vessel: 3 × 10 3 Cells/ml
- No. of vessels per concentration (replicates): 3
- No. of vessels per control (replicates): 3
- No. of vessels per vehicle control (replicates): No data available
- Light intensity and quality: All test tubes were incubated with agitation under constant light intensity (60–120 µE/s/m2).
- Test concentrations: 10-10,000 mg/l
- Results used to determine the conditions for the definitive study: Inhibitions of biomass and growth
Reference substance (positive control):
not specified
Key result
Duration:
72 h
Dose descriptor:
EC50
Effect conc.:
1 065 mg/L
Nominal / measured:
nominal
Conc. based on:
test mat.
Basis for effect:
other: Growth rate and biomass
Remarks on result:
other: 95% confidence interval 999-1150 mg/l
Details on results:
No data available
Results with reference substance (positive control):
No data available
Reported statistics and error estimates:
Growth rate were calculated by linear regression analyses of the logarithmic of algal growth curves for each concentration of chemical and for controls.

In vivo chlorophyll fluorescence was measured daily in a filter fluorometer

Validity criteria fulfilled:
not specified
Conclusions:
Based on the inhibition of growth rate and Area under the growth curves (biomass), the EC50 of chemical 3-Amino-1-methylaminopropane on the Skeletonema costatum was 1065 mg/l.
Executive summary:

Short term toxicity study of N-Methyl-1,3-propanediamine on the growth of marine algae Skeletonema costatum for 72 hrs. Test was performed by static system. The choice of test concentrations was based on a preliminary test with ten-fold dilutions of the test substance (10–10,000 mg/l), in triplicates.

 

Based on the inhibition of growth rate and Area under the growth curves (biomass), the EC50 of chemical 3-Amino-1-methylaminopropane on the Skeletonema costatum was 1065 mg/l. With 95% confidence interval (999-1150).

 

Thus it was concluded that the chemical N-Methyl-1,3-propanediamine was nontoxic and can be consider to be not classified as per the CLP classification criteria.

Description of key information

Short term toxicity study of N-Methyl-1,3-propanediamine on the growth of marine algae Skeletonema costatum for 72 hrs. Test was performed by static system. The choice of test concentrations was based on a preliminary test with ten-fold dilutions of the test substance (10–10,000 mg/l), in triplicates.

 

Based on the inhibition of growth rate and Area under the growth curves (biomass), the EC50 of chemical 3-Amino-1-methylaminopropane on the Skeletonema costatum was 1065 mg/l. With 95% confidence interval (999-1150).

 

Thus it was concluded that the chemical 3-Amino-1-methyl-aminopropane was nontoxic and can be consider to be not classified as per the CLP classification criteria.

Key value for chemical safety assessment

EC50 for marine water algae:
1 065 mg/L

Additional information

Based on the various experimental data and prediction data for the target chemical study have been reviewed to determine the toxic nature of (3-aminopropyl)(methyl)amine (6291-84-5) on the growth rate and biomass of algae. The studies are as mentioned below:

 

In the first key study for the target chemical (3-aminopropyl)(methyl)amine (6291-84-5) International Journal of Greenhouse Gas Control, 2012. Short term toxicity study of N-Methyl-1,3-propanediamine on the growth of marine algae Skeletonema costatum for 72 hrs. Test was performed by static system. The choice of test concentrations was based on a preliminary test with ten-fold dilutions of the test substance (10–10,000 mg/l), in triplicates. Based on the inhibition of growth rate and Area under the growth curves (biomass), the EC50 of chemical 3-Amino-1-methylaminopropane on the Skeletonema costatum was 1065 mg/l. With 95% confidence interval (999-1150). Thus it was concluded that the chemical 3-Amino-1-methyl-aminopropane was nontoxic and can be consider to be not classified as per the CLP classification criteria.

 

Similarly in the second experimental supporting study for (3-aminopropyl)(methyl)amine, from Energy Procedia 2009, Short term toxicity study of N-Methyl-1,3-propanediamine on the growth of marine algae Skeletonema costatum for 72 hrs. Test was performed by static system. The choice of test concentrations was based on a preliminary test with ten-fold dilutions of the test substance (10–10,000 mg/l), performed in triplicates. Based on the inhibition of growth rate and Area under the growth curves (biomass), the EC50 of chemical 3-Amino-1-methylaminopropane on the Skeletonema costatum was 1500 mg/l. Thus it was concluded that the chemical 3-Amino-1-methyl-aminopropane was nontoxic and can be consider to be not classified as per the CLP classification criteria.

 

In the third predicted supporting study for the target chemical (3-aminopropyl)(methyl)amine, from EPISUITE, 2017. Based on the prediction done using the EPI Suite ECOSAR version 1.10, the short term toxicity on green algae was predicted for test substance N-Methyl-1,3- propanediamine. On the basis of effects observed in a static freshwater system, the effect concentration EC50 value for the substance is estimated to be 2129.914 mg/l for green algae for 72 hrs. Based on this value, it can be concluded that the test chemical N-Methyl-1,3-propanediamine can be considered as non-toxic to green algae at environmentally relevant concentrations and can be considered not-classified as per the CLP classification criteria.

 

Thus based on the above data sources for target chemical (3-aminopropyl)(methyl)amine from various experimental studies International Journal of Greenhouse Gas Control, 2012, Energy Procedia 2009, and predicted data, it was summarized that the chemical was nontoxic and can be consider to be not classified as toxic as per the CLP classification criteria.