(epoxyethyl)benzene

Genetic toxicity: in vivo

Administrative data

Endpoint:

in vivo mammalian germ cell study: cytogenicity / chromosome aberration

Remarks:

Type of genotoxicity: chromosome aberration

Type of information:

experimental study

Adequacy of study:

key study

Reliability:

2 (reliable with restrictions)

Rationale for reliability incl. deficiencies:

other: Acceptable, well documented publication which meets basic scientific principles

Cross-reference

Reason / purpose for cross-reference:

reference to same study

Data source

Reference

Reference Type:

publication

Title:

Mutagenicity tests with styrene oxide in mammals.

Author:

Fabry L et al

Year:

1978

Bibliographic source:

Mutat Res. 1978 Sep;51(3):377-81.

Materials and methods

Test guideline

Qualifier:

equivalent or similar to guideline

Guideline:

OECD Guideline 478 (Genetic Toxicology: Rodent Dominant Lethal Test)

GLP compliance:

no

Remarks:

study performed before implementation of GLP

Type of assay:

rodent dominant lethal assay

Test material

Test material information

Constituent 1

Reference substance name:

(epoxyethyl)benzene

EC Number:

202-476-7

EC Name:

(epoxyethyl)benzene

Cas Number:

96-09-3

Molecular formula:

C8H8O

IUPAC Name:

2-phenyloxirane

Test animals

Species:

mouse

Strain:

Balb/c

Sex:

male

Administration / exposure

Route of administration:

intraperitoneal

Vehicle:

Paraffin oil

Details on exposure:

To test the ability of styrene oxide to induce chromosomal aberrations in male post-meiotic germ cells, each male was caged, after injection, with three virgin females from the same strain which were replaced after 7 and 14 days.

Duration of treatment / exposure:

250 mg/kg bw

Frequency of treatment:

once prior to mating

Doses / concentrations

Remarks:

Doses / Concentrations:250 mg/kg bwBasis:no data

No. of animals per sex per dose:

7 treated animals; 10 control animals

Control animals:

yes, concurrent vehicle

Examinations

Tissues and cell types examined:

The males were then kept for another two months, killed and their dividing spermatocytes examined for the presence of reciprocal translocations induced in the pre-meiotic cells. The females were dissected 17 days after mating had started, and pre- and post-implantation losses were determined by conventional methods.

Results and discussion

Test results

Sex:

male

Genotoxicity:

negative

Toxicity:

not specified

Vehicle controls validity:

valid

Negative controls validity:

not applicable

Positive controls validity:

not specified

Applicant's summary and conclusion

Conclusions:

Interpretation of results (migrated information): negativeStyrene oxide did not induce dominant lethal mutations or translocations in meiotic germ cells of male BALB/c mice

Executive summary:

Styrene oxide did not induce dominant lethal mutations or translocations in meiotic germ cells of male BALB/c mice administered styrne oxide by intraperitoneal injection at a dose of 250 mg/kg bw.