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EC number: 229-657-3 | CAS number: 6641-64-1
- Life Cycle description
- Uses advised against
- Endpoint summary
- Appearance / physical state / colour
- Melting point / freezing point
- Boiling point
- Density
- Particle size distribution (Granulometry)
- Vapour pressure
- Partition coefficient
- Water solubility
- Solubility in organic solvents / fat solubility
- Surface tension
- Flash point
- Auto flammability
- Flammability
- Explosiveness
- Oxidising properties
- Oxidation reduction potential
- Stability in organic solvents and identity of relevant degradation products
- Storage stability and reactivity towards container material
- Stability: thermal, sunlight, metals
- pH
- Dissociation constant
- Viscosity
- Additional physico-chemical information
- Additional physico-chemical properties of nanomaterials
- Nanomaterial agglomeration / aggregation
- Nanomaterial crystalline phase
- Nanomaterial crystallite and grain size
- Nanomaterial aspect ratio / shape
- Nanomaterial specific surface area
- Nanomaterial Zeta potential
- Nanomaterial surface chemistry
- Nanomaterial dustiness
- Nanomaterial porosity
- Nanomaterial pour density
- Nanomaterial photocatalytic activity
- Nanomaterial radical formation potential
- Nanomaterial catalytic activity
- Endpoint summary
- Stability
- Biodegradation
- Bioaccumulation
- Transport and distribution
- Environmental data
- Additional information on environmental fate and behaviour
- Ecotoxicological Summary
- Aquatic toxicity
- Endpoint summary
- Short-term toxicity to fish
- Long-term toxicity to fish
- Short-term toxicity to aquatic invertebrates
- Long-term toxicity to aquatic invertebrates
- Toxicity to aquatic algae and cyanobacteria
- Toxicity to aquatic plants other than algae
- Toxicity to microorganisms
- Endocrine disrupter testing in aquatic vertebrates – in vivo
- Toxicity to other aquatic organisms
- Sediment toxicity
- Terrestrial toxicity
- Biological effects monitoring
- Biotransformation and kinetics
- Additional ecotoxological information
- Toxicological Summary
- Toxicokinetics, metabolism and distribution
- Acute Toxicity
- Irritation / corrosion
- Sensitisation
- Repeated dose toxicity
- Genetic toxicity
- Carcinogenicity
- Toxicity to reproduction
- Specific investigations
- Exposure related observations in humans
- Toxic effects on livestock and pets
- Additional toxicological data
Eye irritation
Administrative data
- Endpoint:
- eye irritation: in vivo
- Type of information:
- experimental study
- Adequacy of study:
- weight of evidence
- Reliability:
- 2 (reliable with restrictions)
- Rationale for reliability incl. deficiencies:
- data from handbook or collection of data
- Justification for type of information:
- data is from authoritative databases
Data source
Reference
- Reference Type:
- other: Authoritative database
- Title:
- HSDB for the CAS: 99-54-7
- Author:
- U S National Library of Medicine
- Year:
- 2 008
- Bibliographic source:
- Hazardous Substance DataBank [HSDB], U S National Library of Medicine, last updated 2008
Materials and methods
Test guideline
- Qualifier:
- according to guideline
- Guideline:
- other: as mentioned below
- Principles of method if other than guideline:
- To assess the ocular irritation potential of 1,2-Dichloro-4-nitrobenzene in rabbits
- GLP compliance:
- not specified
Test material
- Reference substance name:
- 1,2-dichloro-4-nitrobenzene
- EC Number:
- 202-764-2
- EC Name:
- 1,2-dichloro-4-nitrobenzene
- Cas Number:
- 99-54-7
- Molecular formula:
- C6H3Cl2NO2
- IUPAC Name:
- 1,2-Dichloro-4-nitrobenzene
- Test material form:
- solid: particulate/powder
- Details on test material:
- Name of the test chemical: 1,2-Dichloro-4-nitrobenzene
Molecular Formula: C6H3Cl2NO2
Molecular Weight: 192.001 g/mol
InChI: 1S/C6H3Cl2NO2/c7-5-2-1-4(9(10)11)3-6(5)8/h1-3H
Substance Type: Organic
Physical State: Solid
Constituent 1
- Specific details on test material used for the study:
- Name of the test chemical: 1,2-Dichloro-4-nitrobenzene
Molecular Formula: C6H3Cl2NO2
Molecular Weight: 192.001 g/mol
InChI: 1S/C6H3Cl2NO2/c7-5-2-1-4(9(10)11)3-6(5)8/h1-3H
Substance Type: Organic
Physical State: Solid
Test animals / tissue source
- Species:
- rabbit
- Strain:
- other: albino
- Details on test animals or tissues and environmental conditions:
- Sex: male and female
Test system
- Vehicle:
- unchanged (no vehicle)
- Controls:
- not specified
- Amount / concentration applied:
- 0.1 ml
- Duration of treatment / exposure:
- single exposure
- Observation period (in vivo):
- 5 days
- Duration of post- treatment incubation (in vitro):
- no data available
- Number of animals or in vitro replicates:
- 3 (1 female and 2 males)
- Details on study design:
- no data available
Results and discussion
In vitro
- Other effects / acceptance of results:
- no data available
In vivo
Results
- Irritation parameter:
- overall irritation score
- Basis:
- mean
- Time point:
- other: 5 days
- Reversibility:
- fully reversible within: 5 days
- Remarks on result:
- probability of weak irritation
- Irritant / corrosive response data:
- Signs of irritation included moderate immediate discomfort, lacrimation together with moderate erythema and edema, and a slight cloudiness of the corneal area. A slight degree of inflammation was present in 1 rabbit after 72-hour. These effects were completely reveresible in 5 days
Applicant's summary and conclusion
- Interpretation of results:
- other: not irritating
- Conclusions:
- Signs of irritation observed in rabbits included moderate immediate discomfort, lacrimation together with moderate erythema and edema, and a slight cloudiness of the corneal area. A slight degree of inflammation was present in 1 rabbit after 72-hour.These effects were fully reversible in 5 days.
Since the observed effects were reversible in 5 days,1,2-Dichloro-4-nitrobenzene can be considered to be not irritating to rabbit eyes. - Executive summary:
The ocular irritation potential of 1,2-Dichloro-4-nitrobenzene was assessed in rabbits.
Undiluted 0.1 ml 1,2-Dichloro-4-nitrobenzene was instilled in the conjunctival sac of the right eye of 3 albino rabbits (1 female and 2 males). The rabbit were observed for signs of irritation till 5 days.Signs of irritation observed in rabbits included moderate immediate discomfort, lacrimation together with moderate erythema and edema, and a slight cloudiness of the corneal area. A slight degree of inflammation was present in 1 rabbit after 72-hour.These effects were fully reversible in 5 days.
Since the observed effects were reversible in 5 days,1,2-Dichloro-4-nitrobenzene can be considered to be not irritating to rabbit eyes.
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