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Ecotoxicological information

Endpoint summary

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Description of key information

Short term toxicity to aquatic invertebrates:

Based on the average value of both models ( Leadscope Enterprise model and SciMatics SciQSAR model), the result were predicted in Battery model. Based on the Danish (Q)SAR Database, the 48 hours EC50 was estimated to be 4.915 mg/l on Daphnia Magna for substance Phenethyl salicylate with immobilization effects. Thus based on this value it can be concluded that the substance can be classified as toxic and can be classified in aquatic chronic 2 category as per the criteria of CLP regulation.

Toxicity to aquatic algae and cyanobacteria:

Based on the prediction done using the OECD QSAR toolbox version 3.4 with log kow as the primary descriptor and considering the seven closest read across substances, the toxicity on Pseudokirchneriella subcapitata (previous names: Raphidocelis subcapitata, Selenastrum capricornutum) was predicted for Target substance Phenethyl Salicylate (87-22-9). EC50 value was estimated to be 3.4957 mg/l for Species Pseudokirchneriella subcapitata (previous names: Raphidocelis subcapitata, Selenastrum capricornutum) for 48 hrs duration when exposed to Phenethyl Salicylate.

Based on this value it can be concluded that the substance Phenethyl Salicylate is considered as toxic to aquatic environment as per the criteria mentioned in CLP regulation. 

Additional information

Summarized results of toxic effect of the chemical Phenethyl Salicylate (87-22-9) 2-phenylethyl 2-hydroxybenzoate on the growth and other normal activities of aquatic lifes, by the collection of data from various databases for target chemical and RA chemicals for evaluating the effects of chemical on the basis of structure similarities.

Short term toxicity to aquatic invertebrates:

Based on the various experimental data and predicted data for the target chemicalPhenethyl Salicylate studyhave been reviewed to determine the toxic nature ofPhenethyl Salicylate. The studies are as mentioned below:

 

In the first weight of evidence study, for Phenethyl Salicylate (87-22-9) 2017, Based on the average value of both models ( Leadscope Enterprise model and SciMatics SciQSAR model), the result were predicted in Battery model. Based on the Danish (Q)SAR Database, the 48 hours EC50 was estimated to be 4.915 mg/l on Daphnia Magna for substance Phenethyl salicylate with immobilization effects. Thus based on this value it can be concluded that the substance can be classified as toxic and can be classified in aquatic chronic 2 category as per the criteria of CLP regulation.

 

In another weight of evidence study for the for target chemical (87-22-9), ACD labs predicted median Lethal Concentration (LC50) of daphnia magna using v5.0.0.184. The value is supported by estimated Reliability Index (RI). Based on this, the LC50 value for test item Phenethyl salicylate was predicted to be 1.2 mg/l for Daphnia magna. On the basis of predicted value it can be concluded that the substance Phenethyl salicylate is considered to be toxic to aquatic environment and can be classified in aquatic chronic 2 as per the criteria mentioned in CLP regulation.

Similarly third weight of evidence study for target chemical (87-22-9), QSAR 2017. Based on the prediction done using the OECD QSAR toolbox version 3.4 with log kow as the primary descriptor and considering the five closest read across substances, the short term toxicity on daphnia magna was predicted for Target substance Phenethyl Salicylate (87-22-9). EC50 value was estimated to be 3.4957 mg/l for Species daphnia magna for 48 hrs duration when exposed to Phenethyl Salicylate. Based on this value it can be concluded that the substance Phenethyl Salicylate is considered as toxic to aquatic environment as per the criteria mentioned in CLP regulation.

Similarly in the fourth weight of evidence study for RA chemical Butyl benzyl phthalate (86-58-7) which takes on the basis of structurally similarities from HSDB supports the toxic classification of chemical. Determination of short term toxicity of Butyl benzyl phthalate to daphnia magna for 24 hrs. Experiment performed by static method, to evaluate the toxicity and also other changes on the growth and movement of daphnia magna. After 24 hrs test organism unable to move, they was in the state of immobility and the effect concentration EC50 was 3.8 mg/l. Based on the immobilization and intoxication of daphnia magna due to the contact with chemical the EC50 was 3.8 mg/l. and thus Butyl benzyl phthalate was toxic and can be consider in aquatic chronic category 2 as per the CLP classification criteria.

 

By considering another RA chemical i.e Fifth weight of evidence study was Phenyl salicylate (118-55-8) from GSBL, 2017. Determination of short term toxicity of Phenyl salicylate to Aplexa hypnorum for 96 hrs. Static method was used for the study and total 20 Aplexa hypnorum was added in each test vessel including control also. Effect was measured in the interval of 24 hrs. Based on the mortality of daphnia magna due to the Phenyl salicylate, the LC50 was 3.44 mg/l.

 

Thus based on the data for target chemicalPhenethyl Salicylate (87-22-9) from various data bases, Phenethyl Salicylate was consider as toxic and can be classified in aquatic chronic 2 category as per the CLP classification criteria.

 

Toxicity to aquatic algae and cyanobacteria:

Based on the various experimental data and predicted data for the target chemicalPhenethyl Salicylate studyhave been reviewed to determine the toxic nature ofPhenethyl Salicylate. The studies are as mentioned below:

 

In the first weight of evidence study, for Phenethyl Salicylate (87-22-9) 2017. Based on the prediction done using the OECD QSAR toolbox version 3.4 with log kow as the primary descriptor and considering the seven closest read across substances, the toxicity on Pseudokirchneriella subcapitata (previous names: Raphidocelis subcapitata, Selenastrum capricornutum) was predicted for Target substance Phenethyl Salicylate (87-22-9). EC50 value was estimated to be 3.4957 mg/l for Species Pseudokirchneriella subcapitata (previous names: Raphidocelis subcapitata, Selenastrum capricornutum) for 48 hrs duration when exposed to Phenethyl Salicylate. Based on this value it can be concluded that the substance Phenethyl Salicylate is considered as toxic to aquatic environment as per the criteria mentioned in CLP regulation.

 

In another weight of evidence study for the for RA chemical Dibenzyl ether (103-50-4) from J-check, 2017 Toxicity of Dibenzyl ether to aquatic algae and cyanobacteria was determined for 72 hrs. Based on the inhibition of growth rate and area under the growth curve of green algae for 72 hrs, the EC50 was 4.1 mg/l, 1.6 mg/l and NOEC was 0.32 mg/l. Based on the EC50 concentration Dibenzyl ether was toxic and can be consider as classified in aquatic chronic category 2 as per CLP classification criteria.

 

In the third weight of evidence study for another RA chemical for benzyl benzoate (118-58-1), 2003 Evaluation of the toxic effect of benzyl salicylate on the growth of freshwater alga, Selenastrum capricornutum. Control and stability sample was also use with the incubation period of 72hrs. Results observed at the interval of 24hrs. An 8.0 mg/L stock solution was prepared by weighing 0.0160 g of test substance into a 2,000 mL Class A glass volumetric flask. Nominal and measured concenyration was taken in the experiment nominal mg/L (control), 0.50, 1.0, 2.0, 4.0, and 8.0 mg/L and Measured: 0.502, 1.01, 1.92, 3.85, and 7.39 mg/L. After the 72hrs exposure the EC50 was 0.735 mg/l, 1.29 mg/l, 0.691 mg/l on the basis of, no of cells/ml, average specific growth rate, and the area under the growth curve. And NOEC was 0.502 mg/l. Based on the EC50 on the basis of average specific growth rate chemical was toxic to algae and can be classified in aquatic chronic 2 category.

 

Similarly data for another RA chemical for Butyl benzyl phthalate (86-58-7) from ecotox databases 2017. Study of inhibition and reduction of Chlorophyll A concentration of Pseudokirchneriella Subcapitata, due to the Butyl benzyl phthalate for 48 hrs. Based on the reduction in chlorophyll concentration the EC50 was 2.71 mg/l. Thus chemical can be consider as toxic and can be consider in aquatic chronic 2 category as per the CLP classification criteria.

 

Thus based on the data for target chemicalPhenethyl Salicylate (87-22-9) from various data bases, Phenethyl Salicylate was consider as toxic and can be classified in aquatic chronic 2 category as per the CLP classification criteria.