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Please be aware that this old REACH registration data factsheet is no longer maintained; it remains frozen as of 19th May 2023.

The new ECHA CHEM database has been released by ECHA, and it now contains all REACH registration data. There are more details on the transition of ECHA's published data to ECHA CHEM here.

Diss Factsheets

Toxicological information

Immunotoxicity

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Administrative data

Endpoint:
immunotoxicity: inhalation
Type of information:
experimental study
Adequacy of study:
other information

Data source

Reference
Reference Type:
publication
Title:
Effects of phosgene exposure on bacterial, viral, and neoplastic susceptibility in mice
Author:
Selgrade MK, Starnes DM, Illing JW, Daniels MJ & Graham JA
Year:
1989
Bibliographic source:
Inhal. Toxicol. 1: 243-259

Materials and methods

Principles of method if other than guideline:
Method: Immunotoxicity/viral susceptability test
GLP compliance:
not specified

Test material

Constituent 1
Chemical structure
Reference substance name:
Phosgene
EC Number:
200-870-3
EC Name:
Phosgene
Cas Number:
75-44-5
Molecular formula:
CCl2O
IUPAC Name:
phosgene

Test animals

Species:
mouse
Strain:
CD-1
Sex:
female

Administration / exposure

Route of administration:
inhalation: gas
Vehicle:
other: nitrogen
Duration of treatment / exposure:
4 h
Frequency of treatment:
single
Doses / concentrations
Remarks:
Doses / Concentrations:
0.025, 0.05 or 0.1 ppm
Basis:
nominal conc.
No. of animals per sex per dose:
40
Control animals:
yes, sham-exposed

Results and discussion

Any other information on results incl. tables

A single 4 h exposure to 0.025, 0.05 or 0.1 ppm phosgene (0.1, 0.2 or 0.4 mg/m3) significantly enhanced mortality due to aerosol infection with Streptococcus zooepidemicus in female mice. Bacteria recovered from lavage fluid of mice exposed to 0.05 ppm for 4 h increased dramatically between 3 and 48 h post infection, while bacteria recovered from lavage fluid of air exposed mice decline to nearly undetectable levels over the same period. When exposure time was increased from 4 to 8 h, significant effects on mortality due to streptococcus infection were observed at 0.01 ppm phosgene. A 4 h exposure to 0.025 ppm phosgene also caused a significant increased in the number of B16 melanoma tumours that developed in the lungs of mice following intravenous inoculation of syngeneic tumour cells. The data indicate that exposure to phosgene concentration as low as 0.025 ppm significantly enhanced susceptibility to bacterial and tumour disease in mice.

Applicant's summary and conclusion