Amines, N-(3- aminopropyl)-N’-[3-(C18 and C18-unsatd. alkyl amino)propyl]trimethylenedi and amines, N-(3-aminopropyl)-N’-(C18 and C18-unsatd. alkyl)trimethylenedi-

Ecotoxicological Summary

Hazard for aquatic organisms

Freshwater

Hazard assessment conclusion:

PNEC aqua (freshwater)

PNEC value:

10.4 µg/L

Assessment factor:

50

Extrapolation method:

assessment factor

PNEC freshwater (intermittent releases):

6.84 µg/L

Marine water

Hazard assessment conclusion:

PNEC aqua (marine water)

PNEC value:

1.04 µg/L

Assessment factor:

500

Extrapolation method:

assessment factor

PNEC marine water (intermittent releases):

0.684 µg/L

STP

Hazard assessment conclusion:

PNEC STP

PNEC value:

2.8 mg/L

Assessment factor:

10

Extrapolation method:

assessment factor

Sediment (freshwater)

Hazard assessment conclusion:

PNEC sediment (freshwater)

PNEC value:

41.4 mg/kg sediment dw

Assessment factor:

1

Extrapolation method:

equilibrium partitioning method

Sediment (marine water)

Hazard assessment conclusion:

PNEC sediment (marine water)

PNEC value:

4.14 mg/kg sediment dw

Assessment factor:

1

Extrapolation method:

equilibrium partitioning method

Hazard for air

Air

Hazard assessment conclusion:

no hazard identified

Hazard for terrestrial organisms

Soil

Hazard assessment conclusion:

PNEC soil

PNEC value:

10 mg/kg soil dw

Assessment factor:

100

Extrapolation method:

assessment factor

Hazard for predators

Secondary poisoning

Hazard assessment conclusion:

PNEC oral

PNEC value:

0.56 mg/kg food

Assessment factor:

90

Additional information

Since the linear N-C16-18 (evennumbered) C18 unsaturated dipropylene triamines and tripropylenetetraamines are poorly soluble in water, and as they are positively charged under environmental conditions, it is very likely that they adsorb to soil, sediment and other negatively charged surfaces. The results of ecotoxicity testing in synthetic growth media are therefore influenced by secondary effects like sorption to glassware. In order to reduce the influence of these secondary effects, modifications such as river water consitituents (or humic acids) can be introduced as stabilizer of the test substances which limit the sorption glassware and at the same time creates more realistic environmental conditions. These studies should therefore be considered as higher tier studies.

For the aquatic risk assessment of strongly sorbing substances the current REACH Guidance Documents do not provide sufficient guidance concerning both effects and exposure assessment. The best and most realistic alternative for strongly sorbing substances is the PEC/PNEC_{aquatic bulk} approach (ECETOC 2003). This approach is based on a PNEC_{aquatic bulk} which is derived from a modified ecotoxicity test using humic acid, natural water or effluent and a PEC_{local, aquatic bulk} which represents the total aquatic concentration (dissolved and sorbed = bulk). The risk quotient for the aquatic compartment is calculated by using nominal concentrations.

Conclusion on classification

For risk assessment the bulk approach data can be used directly but for classification, the river water data are corrected with a factor of 10 to compensate for the mitigation by river water constituents. This means that it is assumed that 90% of the substance will not be available in the test to cause any effects. When calculating the fraction sorbed using the standard equations (only based on hydrophobic sorption to organic matter) only 43% is assumed to be sorbed (in surface water).

The data used for the acute classification are the corrected ErC50 of 0.109 mg/L and the corrected EC50 adult mortality of 0.0684 mg/L. The lowest value is within the range: 0.01 < L(E)C50≤0.1. This leads to an acute 1 classification and an M-factor of 10.

 The data used for the chronic classification are the corrected ErC10 of 0.0518 mg/L and the corrected EC10 of 0.0577 mg/L. The lowest value is within the range: 0.01 < EC10≤0.1

This leads to a chronic 2 classification and an M-factor of 1 because the substance is not considered to be readily biodegradable.