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Please be aware that this old REACH registration data factsheet is no longer maintained; it remains frozen as of 19th May 2023.

The new ECHA CHEM database has been released by ECHA, and it now contains all REACH registration data. There are more details on the transition of ECHA's published data to ECHA CHEM here.

Diss Factsheets

Administrative data

Endpoint:
immunotoxicity, other
Remarks:
in vitro
Type of information:
experimental study
Adequacy of study:
supporting study
Reliability:
4 (not assignable)
Rationale for reliability incl. deficiencies:
documentation insufficient for assessment

Data source

Reference
Reference Type:
publication
Title:
Immunotoxicological evaluation of environmental chemicals utilizing mouse lymphocyte mitogenesis test.
Author:
Sakazaki, H., Ueno, H., Umetani, K., Utsumi, H. and Nakamuro, K.
Year:
2001
Bibliographic source:
J. Health Sci., 47(3):258-271.

Materials and methods

Principles of method if other than guideline:
no guideline reported
GLP compliance:
not specified

Test material

Constituent 1
Chemical structure
Reference substance name:
o-phenylenediamine
EC Number:
202-430-6
EC Name:
o-phenylenediamine
Cas Number:
95-54-5
Molecular formula:
C6H8N2
IUPAC Name:
benzene-1,2-diamine

Results and discussion

Any other information on results incl. tables

B Cell IC50 = 1x10E-5 mol/L
Inhibitory Pattern: D

T Cell
Inhibitory Pattern: A

Pattern A = chemical showed no inhibition within the concentration range assayed.

Pattern D = inhibition occurred suddenly at certain concentrations, probably by toxicity.

Pattern A was judged negative. Pattern D was judged positive.

 

Applicant's summary and conclusion

Conclusions:
Positive in B cell
Negative in T cell
Executive summary:

A lymphocyte mitogenesis test was conducted with o-phenylenediamine. The growth inhibitory effect of oPDA on mouse splenic lymphocytic mitogenesis was assessed using lipopolysacchardie and concanavalin A as the specific mitogen for B cells and T cells. The DNA content of grown cells was determined by the novel ethidium bromide-fluorophotometry method, with 96-well microplates. The test substance showed inhibition suddenly at certain concentrations in B cell (probably by toxicity), but showed no inhibition in T cell.