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Ecotoxicological information

Short-term toxicity to aquatic invertebrates

Administrative data

Endpoint:
short-term toxicity to aquatic invertebrates
Type of information:
experimental study
Adequacy of study:
key study
Reliability:
1 (reliable without restriction)
Rationale for reliability incl. deficiencies:
guideline study

Data source

Reference
Reference Type:
study report
Title:
Unnamed
Year:
2016

Materials and methods

Test guidelineopen allclose all
Qualifier:
according to
Guideline:
OECD Guideline 202 (Daphnia sp. Acute Immobilisation Test)
Qualifier:
equivalent or similar to
Guideline:
EU Method C.2 (Acute Toxicity for Daphnia)
GLP compliance:
yes (incl. certificate)

Test material

Reference
Name:
Unnamed
Type:
Constituent
Test material form:
solid

Sampling and analysis

Analytical monitoring:
yes
Remarks:
HPLC-UV

Test organisms

Test organisms (species):
Daphnia magna
Details on test organisms:
Daphnia magna (Straus) clone 5, aged from 0.75 to 17.5 hours old, female.
The daphnids introduced in the test were taken from ibacon's in-house laboratory culture

Study design

Test type:
static
Water media type:
other: Reconstituted Water
Remarks:
based on the OECD 202 water composition
Limit test:
yes
Total exposure duration:
48 h
Post exposure observation period:
Not specified

Test conditions

Hardness:
2.5 mmol/L (= 250 mg/L) as CaCO3
Test temperature:
Water temperature of 20°C.
pH:
8.0 to 8.4
Dissolved oxygen:
7.1 to 8.7 mg/L
Nominal and measured concentrations:
Test concentration of nominal 100 mg test item/L, corresponding to a mean measured test concentration of 63.3 mg test item/L.
Details on test conditions:
Photoperiod: 16 h light - 8 h dark; light intensity: 500 to 760 lux.
Reference substance (positive control):
yes
Remarks:
potassium dichromate

Results and discussion

Effect concentrationsopen allclose all
Key result
Duration:
48 h
Dose descriptor:
NOEC
Effect conc.:
>= 63.3 mg/L
Nominal / measured:
meas. (geom. mean)
Conc. based on:
test mat.
Basis for effect:
mobility
Key result
Duration:
48 h
Dose descriptor:
LOEC
Effect conc.:
> 63.3 mg/L
Nominal / measured:
meas. (geom. mean)
Conc. based on:
test mat.
Basis for effect:
mobility
Key result
Duration:
48 h
Dose descriptor:
EC50
Remarks on result:
not determinable
Details on results:
After 48 hours of exposure, no immobilisation of the test animals was observed in the control and in the only test concentration of 63.3 mg test item/L (mean measured).
The EC50, EC20 and ED10 were not determinable. The EC50 could not be quantified due to the absence of toxicity of the test item. The NOEC and the LOEC were determined directly from the raw data.

Applicant's summary and conclusion

Validity criteria fulfilled:
yes
Conclusions:
Based on the experimental results, for Noroxymorphone, the 48-hour NOEC was determined to be ≥ 63.3 mg test item/L (mean measured). The 48-hour LOEC was determined to be > 63.3 mg test item/L (mean measured) and the 48-hour EC50 value could not be determined.