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EC number: 813-180-8
CAS number: 5856-32-6
The percent biodegradation in the toxicity control, containing
both the test item and the reference item, was calculated based on the
sum of the total organic carbon content (TOC) of the test item and the
In the toxicity control, the CO2 formation over the 19 day
exposure period correlated very well with the CO2 evolution from the
two added substances, i.e. test item and reference item. Within 14
days of exposure, biodegradation amounted to 78 %, reaching 83 % at
the end of the test. Thus, according to the test guidelines, the test
item had no inhibitory effect on activated sludge microorganisms at
the tested concentration of 20.8 mg/L because biodegradation in the
toxicity control was ≥ 25 % within 14 days of incubation.
The tabulated values represent rounded results obtained by
calculation using the exact raw data.
Table 1 Inorganic Carbon (IC Concentrations) Measured in the
Table 2 Absolute Amount of Inorganic Carbon (IC) Produced
Table 3 Biodegradation of the Test Item and the Reference
on values presented in Table 2, corrected for the inoculum controls
Table 4 pH of the Test Solutions or Suspensions
Figure 1: Biodegradation of the Test
Item 10-HSA and the Reference Item Sodium benzoate during the
A: Overview over the whole test series
B: 10-day window for the biodegradation of the test item
(see picture below under section Illustration)
The test item 10-HSA was investigated for its ready biodegradability in
a 28 Day CO2 Evolution (Modified Sturm) Test according to the OECD
Guideline for Testing of Chemicals, No. 301 B (1992), the Method C.4-C
of Commission Regulation (EC) No 440/2008 and the US EPA OPPTS 835.3110
Ready Biodegradability, Paragraph (m) (1998).
In accordance with the test guidelines the test was ended after 19 days
since the biodegradation curves of the test item had reached a plateau
over at least three determinations.
The test item 10-HSA was found to be biodegradable by 85 % under the
test conditions within the 19 day exposure period.
The pass level for ready biodegradability, i.e. a CO2 formation of at
least 60 % of the total organic carbon (TOC) in a 10 day window within
the normally 28 day period of the test, was reached by exposure day 7
(62 %). In conclusion, 10-HSA was found to be readily biodegradable
under the test conditions within 19 days.
In the toxicity control, containing both 10-HSA and the reference item
sodium benzoate, biodegradation reached 78 % of the overall added TOC
within 14 days of exposure. Thus, 10-HSA had no inhibitory effect on the
activity of activated sludge microorganisms at the tested concentration
of 20.8 mg/L.
In the procedure controls, average biodegradation of the reference item
was 72 % by Exposure Day 5, and 90 % by Exposure Day 14, thus confirming
suitability of the activated sludge (≥ 60 % degradation by Exposure Day
14). By the end of the test (Exposure Day 19), average biodegradation
was 92 %. The validity criteria were fulfilled.
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