2,2-bis(allyloxymethyl)butan-1-ol

Toxicity to microorganisms

Administrative data

Endpoint:

activated sludge respiration inhibition testing

Type of information:

experimental study

Adequacy of study:

key study

Study period:

3 hours

Reliability:

1 (reliable without restriction)

Rationale for reliability incl. deficiencies:

other: Test was performed according to OECD test guidelines, and a statement of GLP compliance has been made.

Data source

Reference

Reference Type:

study report

Title:

Unnamed

Year:

2010

Report date:

2010

Materials and methods

Test guideline

Qualifier:

according to guideline

Guideline:

OECD Guideline 209 (Activated Sludge, Respiration Inhibition Test

Deviations:

no

GLP compliance:

yes (incl. QA statement)

Test material

Test material information

Constituent 1

Reference substance name:

2,2-bis(allyloxymethyl)butan-1-ol

EC Number:

211-661-1

EC Name:

2,2-bis(allyloxymethyl)butan-1-ol

Cas Number:

682-09-7

Molecular formula:

C12H22O3

IUPAC Name:

2,2-bis[(prop-2-en-1-yloxy)methyl]butan-1-ol

Details on test material:

- Name of test material (as cited in study report): TMPDE
- Lot/batch No.: 3852003
- Purity: 91.6%

Specific details on test material used for the study:

Details on properties of test surrogate or analogue material (migrated information):
Not applicable

Sampling and analysis

Analytical monitoring:

no

Details on sampling:

Not applicable

Test solutions

Vehicle:

no

Details on test solutions:

A 5 g TMPDE /L stock emulsion was prepared by adding TMPDE (5.0000 g) to a 1000 mL volumetric flask and bringing to volume with deionised water. The solution was ultrasonicated for ca 15 min and then shaken by hand for a few min resulting in a fine uniform emulsion.

Test organisms

Test organisms (species):

activated sludge of a predominantly domestic sewage

Details on inoculum:

A sample of activated sewage sludge was obtained from Haddington Municipal Sewage Works (a local sewage processing plant, which handles predominantly domestic sewage) on 06 April 2010. On arrival at the laboratory, the sludge was aerated. The solid content of the sludge was determined by shaking the bottle of sludge by hand to homogenise thoroughly. Duplicate sub-samples (5mL) of the homogenised sludge were dried in an oven at approximately 105°C and the suspended solids content determined as 3.1 g/L. Synthetic sewage feed was then added at a rate of 50 mL/L sludge/day and the sludge left aerating. To concentrate the solid sewage flocculent to the required level, the sludge was allowed to settle before 2 L of the overlying supernant was siphoned off. The solid content of the sludge was re-determined prior to use and found to be 4.2 g/L, which was acceptable for use. The pH of the sludge was measured as 7.84 upon receipt.

Study design

Test type:

static

Water media type:

freshwater

Limit test:

no

Total exposure duration:

3 h

Post exposure observation period:

Not applicable

Test conditions

Hardness:

Not applicable

Test temperature:

18.9 to 19.5°C

pH:

The pH of the sludge was measured as 7.84 upon receipt.

Dissolved oxygen:

Measured during the study.

Salinity:

Not applicable

Nominal and measured concentrations:

Nominal exposure concentrations were 10, 31.6, 100, 316 and 1000 mg/L.

Reference substance (positive control):

yes

Remarks:

3,5-dichlorophenol

Results and discussion

Effect concentrations

Effect concentrations 1

Duration:

3 h

Dose descriptor:

EC50

Effect conc.:

> 1 000 mg/L

Nominal / measured:

nominal

Conc. based on:

test mat.

Basis for effect:

inhibition of total respiration

Remarks:

respiration rate

Effect concentrations 2

Duration:

3 h

Dose descriptor:

NOEC

Effect conc.:

316 mg/L

Nominal / measured:

nominal

Conc. based on:

test mat.

Basis for effect:

inhibition of total respiration

Remarks:

respiration rate

Results with reference substance (positive control):

The 3 h EC50 for 3,5-DCP to activated sludge was estimated to be 15.23 mg/L with 95 % confidence limits of 12.65 and 18.32 mg/L, under the conditions of this test.

Reported statistics and error estimates:

The respiration rate for each vessel was calculated from the recorded oxygen concentrations and expressed as mg O2/L/h for the period between approximately 6.5 and 2.5 mg O2/L. Where respiration was low the calculation was conducted over the full linear range.

The EC50 and associated 95% confidence interval was estimated using the non-parametric Trimmed Spearman-Karber Method (Hamilton et al, 1977), where appropriate.

Any other information on results incl. tables

Respiration rates and percentage inhibition

Treatment	Nominal concentration (mg/L)	Respiration rate (mg O2/L/h)	% inhibition
Control 1	0	57.60	-
Control 2	0	51.30	-
Mean of controls	-	54.45	-
% difference in controls	-	10.9	-
3,5,-DCP	5	46.68
	15	28.67
	30	12.38
TMPDE	10	59.00	0
	31.6	64.00	0
	100	61.20	0
	316	55.44	0
	1000	34.0	37.44

 

Applicant's summary and conclusion

Validity criteria fulfilled:

yes

Conclusions:

Following three hours exposure to TMPDE the EC50 was observed to be > 1000 mg/L (based on nominal exposure concentrations). A 37% effect on respiration was seen at 1000 mg/L with no effect at 316 mg/L, therefore the NOEC was observed to 316 mg/L.

Executive summary:

The toxicity of TMPDE to activated sewage sludge was investigated in a respiration inhibition test according to OECD test guideline 209. Following three hours exposure to TMPDE the EC50 was observed to be > 1000 mg/L (based on nominal exposure concentrations). A 37% effect on respiration was seen at 1000 mg/L with no effect at 316 mg/L, therefore the NOEC was observed to 316 mg/L.