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Short-term toxicity to fish

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Description of key information

The 96-hour NOEC (highest concentration tested without toxic effects after the exposure period of 96 hours), and the 96-hour LC0 of FAT 60'253/A to zebra fish were determined to be at least 0.0030 mg/l (loading rate of 100 mg/l).

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The acute toxicity of the test item FAT 60'253/A to zebra fish (Brachydanio rerio) was determined in a 96-hour semi-static test with a daily test medium renewal according to the Commission Directive 92/69/EEC, Annex Part C.1 dated December 29, 1992, and the OECD Guideline for Testing of Chemicals No. 203, adopted July 17, 1992.

Due to the very low water solubility of the test item, supersaturated stock dispersions of the test item with a loading rate of nominal 100mg/l were continuously stirred at room temperature in the dark over 7 days. Then, the dispersions were filtered. These filtrates were freshly prepared at each test medium renewal during this semi-static test. The undiluted filtrate with the maximum concentration of dissolved and very finely dispersed test item was used as the only test medium. Additionally, a control was tested in parallel.

The analytically determined test item concentration in the samples from the freshly prepared test medium (the undiluted filtrate of the supersaturated dispersion) amounted to 0.0039 and 0.0029 mg/l at the start of the test. After the test medium renewal period of one day, the measured concentrations of FAT 60'253/A slightly decreased to 0.0035 and 0.0018 mg/l. This slight decrease of the measured test item concentrations might have been caused by a precipitation of the finely dispersed test item, or due to a loss by adsorption onto the glass surfaces. All biological results are related to the mean measured test item concentration of 0.0030 mg/l (calculated as the average over all measurements in the undiluted filtrate during the test period).

In the control and in the undiluted filtrate, no mortality or other signs of intoxication were determined at the test fish during the test period of 96 hours.

Therefore, the 96-hour NOEC (highest concentration tested without toxic effects after the exposure period of 96 hours), and the 96-hour LC0 of FAT 60'253/A to zebra fish were determined to be at least 0.0030 mg/l (loading rate of 100 mg/l). The 96-hour NOEC and the 96-hour LC0 might even be higher but concentrations of the test item above the maximum concentration which could be dissolved or very finely dispersed in the test water were not tested, according to the guidelines. The 96-hour LOEC (lowest concentration with toxic effects), the 96-hour LC50 and the 96-hour LC100 were clearly higher than the solubility limit of the test item in the test water. These values could not be quantified, since the test item had no toxic effect up to the highest concentration, which could be dissolved and very finely dispersed in the test water.