Registration Dossier

Administrative data

Endpoint:
adsorption / desorption: screening
Type of information:
experimental study
Adequacy of study:
key study
Study period:
08 January 2009 to 07 February 2009
Reliability:
1 (reliable without restriction)
Rationale for reliability incl. deficiencies:
other: Study conducted in compliance with agreed protocols, with no or minor deviations from standard test guidelines and/or minor methodological deficiencies, which do not affect the quality of relevant results.

Data source

Reference
Reference Type:
study report
Title:
Unnamed
Year:
2009
Report Date:
2009

Materials and methods

Test guideline
Qualifier:
according to
Guideline:
EU Method C.19 (Estimation of the Adsorption Coefficient (KOC) on Soil and Sewage Sludge Using High Performance Liquid Chromatography (HPLC))
Deviations:
yes
Remarks:
Please see below.
Principles of method if other than guideline:
The method guideline states that the measurement of adsorption coefficient should be carried out on substances in their ionised and unionised forms. However, the dissociation constants of certain functional groups found in the test material made it impossible to satisfy this criteria. Testing would be required at pH <0 and at pH >10 for the acidic and basic functional groups respectively. As a consequence of this, one of the functional groups will always be present in its ionised form. Therefore, as these pH’s are outside the environmental pH range for this test and also experimentally impractical, testing was carried out at approximately neutral pH with the test material being ionised. pH 7 is also the recommended pH for salts of sulphonic acid groups.
GLP compliance:
yes (incl. certificate)
Remarks:
Date of inspection: 19 August 2008; Date of signature: 04 March 2009
Type of method:
HPLC estimation method
Media:
soil/sewage sludge

Test material

Reference
Name:
Unnamed
Type:
Constituent
Details on test material:
Sponsor's identification : H-CB sodium salt
Description : blue solid
Purity : 94.8%
Lot number : MB-2
Date received : 04 December 2008
Storage conditions : room temperature in the dark

Radiolabelling:
not specified

Study design

Test temperature:
Not stated.

Batch equilibrium or other method

Analytical monitoring:
yes

Results and discussion

Any other information on results incl. tables

Calibration

The retention times of formamide and the retention times, capacity factors (k) and log10 Koc values for the reference standards are shown in the two following tables:

Table 2

Dead Time

Retention Time (mins)

Mean Retention Time (mins)

Injection 1

Injection 2

Formamide

3.381

3.376

3.379

Table 3

Standard

Retention Time (mins)

Mean Retention Time (mins)

Capacity Factor (k)

Log10k

Log10Koc

Injection 1

Injection 2

Acetanilide

4.075

4.077

4.076

0.206

-0.685

1.25

Phenol

4.084

4.083

4.084

0.209

-0.681

1.32

Atrazine

4.514

4.507

4.511

0.335

-0.475

1.81

Isoproturon

4.901

4.902

4.902

0.451

-0.346

1.86

Triadimenol

5.276

5.277

5.277

0.562

-0.250

2.40

Linuron

6.158

6.157

6.158

0.823

-8.48 x 10-2

2.59

Naphthalene

7.900

7.900

7.900

1.34

0.127

2.75

Endosulfan-diol

6.090

6.094

6.092

0.803

-9.52 x 10-2

3.02

Fenthion

10.373

10.377

10.375

2.07

0.316

3.31

a-Endosulfan

10.571

10.581

10.576

2.13

0.328

4.09

Phenanthrene

14.078

14.053

14.066

3.16

0.500

4.09

Diclofop-methyl

12.756

12.759

12.758

2.78

0.443

4.20

DDT

30.893

30.959

30.926

8.15

0.911

5.63

 

Adsorption coefficient of sample

The retention times, capacity factors and log10 Koc values determined for the sample are shown in the following table:

Table 4

Injection

Retention Time (mins)

Capacity Factor (k)

Log10k

Log10Koc

1

1.687

<0.206

<-0.685

<1.25

2

1.686

<0.206

<-0.685

<1.25

Mean log10 Koc:                     <1.25
Adsorption coefficient:        <17.8

Applicant's summary and conclusion

Validity criteria fulfilled:
yes
Conclusions:
The adsorption coefficient (Koc) of the test material has been determined to be <17.8, log10 Koc <1.25.
Executive summary:

The Adsorption Coefficient was calculated to be less than 17.8, log10 Koc <1.25 using the HPLC screening method, Method C19 Adsorption Coefficient of Commission Regulation (EC) No 440/2008 of 30 May 2008.