P 16513

Short-term toxicity to aquatic invertebrates

Administrative data

Endpoint:

short-term toxicity to aquatic invertebrates

Type of information:

experimental study

Adequacy of study:

key study

Study period:

2008

Reliability:

1 (reliable without restriction)

Cross-reference

Cross-reference 1

Reason / purpose for cross-reference:

reference to same study

Cross-reference 2

Reason / purpose for cross-reference:

reference to other study

Data source

Reference

Reference Type:

study report

Title:

Unnamed

Year:

2008

Report date:

2008

Materials and methods

Test guideline

Qualifier:

according to guideline

Guideline:

OECD Guideline 202 (Daphnia sp. Acute Immobilisation Test)

Deviations:

no

GLP compliance:

yes

Test material

Test material information

Constituent 1

Reference substance name:

Strontium 4-chloro-2-[2-hydroxy-6-sulfonatonaphthalen-1-yl)diazenyl]benzoate

Cas Number:

474814-88-5

Molecular formula:

C17H9ClN2O6SSr

IUPAC Name:

Strontium 4-chloro-2-[2-hydroxy-6-sulfonatonaphthalen-1-yl)diazenyl]benzoate

Test material form:

solid: nanoform, no surface treatment

Sampling and analysis

Analytical monitoring:

yes

Details on sampling:

- Concentrations: 29,4 mg/l; limit concentration: saturated solution (100 mg/l)

Test solutions

Details on test solutions:

PREPARATION AND APPLICATION OF TEST SOLUTION (especially for difficult test substances)
- Method: the stock solution (100mg/l test item) was shaken with 20rpm for 24 h at ambient temperature. After 24 h undissolved particles were removed by centrifugation for 20 min at 3000 rpm.

Test organisms

Test organisms (species):

Daphnia magna

Details on test organisms:

TEST ORGANISM
- Strain: Straus (Clone 5)

Study design

Test type:

static

Water media type:

freshwater

Limit test:

yes

Total exposure duration:

48 h

Post exposure observation period:

no

Test conditions

Hardness:

160 - 180 mg CaCO3/l

Test temperature:

19-20°C

pH:

7.72-7.95

Dissolved oxygen:

6.50-8.14 mg/l

Nominal and measured concentrations:

nominal: 100 mg/l
mean measured: 29.4 mg/l

Reference substance (positive control):

yes

Remarks:

potassium dichromate

Results and discussion

Effect concentrations

Effect concentrations 1

Duration:

48 h

Dose descriptor:

EC50

Effect conc.:

> 29.4 mg/L

Nominal / measured:

meas. (arithm. mean)

Conc. based on:

test mat.

Basis for effect:

mobility

Effect concentrations 2

Duration:

48 h

Dose descriptor:

LC0

Effect conc.:

> 29.4 mg/L

Nominal / measured:

meas. (arithm. mean)

Conc. based on:

test mat.

Basis for effect:

mortality

Effect concentrations 3

Duration:

48 h

Dose descriptor:

LC100

Effect conc.:

> 29.4 mg/L

Nominal / measured:

meas. (arithm. mean)

Conc. based on:

test mat.

Basis for effect:

mortality

Details on results:

- Behavioural abnormalities: no
- Mortality of control: no

Results with reference substance (positive control):

- Results with reference substance valid? y
- EC50: 1.62 mg/l (CI 1.59-1.65 mg/l)

Applicant's summary and conclusion

Validity criteria fulfilled:

yes

Conclusions:

No effects on Daphnia Magna until the limit of water solubility of 29.4 mg/l;