Registration Dossier
Registration Dossier
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EC number: 202-885-0 | CAS number: 100-74-3
Toxicity to microorganisms
Administrative data
Link to relevant study record(s)
Description of key information
Two Klimisch 2 studies are available and used in a WoE approach, a OECD 209 study and a study performed according to the DIN 38412, part 8 guideline. The OECD 209-test results (BASF, 1987) were chosen to derive a PNEC-STP and not a test result which was not performed according to standardized test method. The respiration inhibition test from BASF was performed according to a valid guideline including a test duration of 30 min. The 30 min. EC50 value of > 600 mg/L is used for the CSA and derivation of the PNEC.
Key value for chemical safety assessment
- EC50 for microorganisms:
- 600 mg/L
Additional information
Two studies were used in a WoE approach are used to cover this endpoint.
The key study, a respiration inhibition study (OECD TG209) by BASF AG (1987) reported a 30-min EC50 of > 600 mg/L. The single concentration tested was 600 mg/L of morpholine, 4 -ethyl-, and a blank control using domestic/synthetic sewage sludge.
A second study study was a Pseudomonas putida oxygen consumption study (BASF, 1990). The study was performed according to (DIN 38412, part 8) and reported a 17-h EC50 of 1768 mg/L. The 17-h EC10 value was 1151 mg/L.
As the Pseudomonas putida oxygen consumption test is not a standard test normally used for this endpoint, the results of the standard OECD 209 were considered as key values for the assessment.
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