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Toxicological information

Genetic toxicity: in vitro

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Administrative data

Endpoint:
in vitro gene mutation study in bacteria
Remarks:
Type of genotoxicity: gene mutation
Type of information:
experimental study
Adequacy of study:
weight of evidence
Reliability:
2 (reliable with restrictions)
Rationale for reliability incl. deficiencies:
other: Data is from peer reviewed journal

Data source

Reference
Reference Type:
publication
Title:
Mutagenecity study of benzidiene and its analogs: Structure activity relationships
Author:
King-Thom chung, Ssu-Ching Chen, Tit Yee Wong, Ying –Sing Li , Cheng-I Wei and Ming W. Chou
Year:
2000
Bibliographic source:
Toxicological sciences 56 351-356(2000)

Materials and methods

Test guideline
Qualifier:
according to
Guideline:
other:
Principles of method if other than guideline:
The Ames Salmonella/ microsome assay
GLP compliance:
not specified
Type of assay:
bacterial gene mutation assay

Test material

Reference
Name:
Unnamed
Type:
Constituent
Details on test material:
- Name of test material (as cited in study report): 2-Aminobiphenyl
- Molecular formula (if other than submission substance): C12-H11-N
- Molecular weight (if other than submission substance): 169.226 g/mol
- Substance type: Organic
- Physical state: colourless to purple
- Purity :No data available
- Impurities (identity and concentrations): No data available

Method

Species / strain
Species / strain / cell type:
S. typhimurium, other: TA 98 and TA 100
Additional strain / cell type characteristics:
not specified
Metabolic activation:
with and without
Metabolic activation system:
Aroclor 1254-induced rat S9 mix
Test concentrations with justification for top dose:
3, 10, 30, 100, 300, ug/plate
Vehicle / solvent:
Water/DMSO
Controls
Untreated negative controls:
not specified
Negative solvent / vehicle controls:
yes
True negative controls:
not specified
Positive controls:
yes
Positive control substance:
2-nitrofluorene
other: 2-aminofluorene and MNNG
Details on test system and experimental conditions:
preincubation assay
Statistics:
The mutagenic potency was calculated by taking the slope of the dose-response curve.

Results and discussion

Test results
Species / strain:
S. typhimurium, other: TA98 and TA100
Metabolic activation:
with and without
Genotoxicity:
negative
Cytotoxicity / choice of top concentrations:
not specified
Vehicle controls validity:
valid
Untreated negative controls validity:
not specified
Positive controls validity:
valid
Remarks on result:
other: all strains/cell types tested
Remarks:
Migrated from field 'Test system'.

Applicant's summary and conclusion

Conclusions:
Interpretation of results (migrated information):
negative

2-Aminobiphenyl is non-mutagenic to the Salmonella typhimurium TA98 and TA100 in the presence and absence of S9 metabolic activation system
Executive summary:

The AMES salmonella assay (preincubation protocol) was employed to test the mutagenecity of 2- Aminobiphenyl using Salmonella typhimurium strains TA98 and TA100 in the presence of aroclor 1254 induced rat S9 mix.

No. of revertant colonies did not increase for the test compound as compared to control.

 

Hence, 2-Aminobiphenyl is non-mutagenic to the Salmonella typhimurium TA98 and TA100 in the presence and absence of S9 metabolic activation system.