Benzimidazole

Water solubility

Link to relevant study record(s)

Reference

Endpoint:

water solubility

Type of information:

experimental study

Adequacy of study:

key study

Study period:

25-02-2017 – 03-03-2017

Reliability:

1 (reliable without restriction)

Rationale for reliability incl. deficiencies:

guideline study

Justification for type of information:

Experimental test result performed using standard OECD test guidelines

Qualifier:

according to guideline

Guideline:

OECD Guideline 105 (Water Solubility)

Principles of method if other than guideline:

The water solubility determination of test item Benzimidazole (CAS No. 51-17-2) was done as per the OECD guideline 105 and by spectrophotometric analytical method as per OECD 101.

GLP compliance:

not specified

Type of method:

other: not specified

Specific details on test material used for the study:

- Name of test material : Benzimidazole
- Molecular formula : C7H6N2
- Molecular weight :118.1384 g/mol
- Smiles notation : c12c(nc[nH]1)cccc2
- InChl : 1S/C7H6N2/c12476(31)8597/h15H,(H,8,9)
- Substance Type: Organic
- Physical State: Solid crystalline powder (Off white)
- Purity: 99.5%

Key result

Water solubility:

6 320.46 mg/L

Conc. based on:

test mat.

Temp.:

30 °C

Remarks on result:

other: no additional details mentioned

WATER SOLUBILITY: PRELIMINARY TEST

0.1g of test item was taken in 100 ml beaker. RO water was added in increasing amount in sequence of 0.1 ml, 0.5 ml, 1.0 ml, 2.0 ml, 10.0 ml and 100.0 ml with intermediate stirring.

For highly soluble substances: Preliminary water solubility of test item was found 0.1g in >100 ml RO water. For analytical determination 0.1 g of test item was dissolved in 100 ml of RO water. The solution was kept for 24hrs on stirring and absorbance was recorded. Concentration detected on the basis of standard curve was 1218.64 mg/L. Five times the concentration recorded from preliminary solubility was used for confirmatory study.

WATER SOLUBILITY: CONFIRMATORY TEST

Five times the concentration recoded from preliminary solubility = 1218.64 mg/L × 5 = 6093.2 mg/L was used for confirmatory study. Two separate solution of 609.32 g in 100 ml RO water were prepared and kept for stirring for 48 hrs and 72 hrs. After the completion of stirring period the absorbance for all the three solution were recorded and concentration was detected on the basis of standard curve.

Sample stirring time (hours)	Concentration obtained on the basis of standard curve (mg/l)	Dilution factor	Solubility value obtained (mg/l)
24	-	-	-
48	17.0215	400	6806.6
72	29.1616	200	6832.32

Considering the water solubility for 48 hrs and 72 hrs the mean water solubility was calculated as follows:

Mean water solubility value for 48 hour run sample + 72 hour run sample

=(6808.6 +5832.32)/2

=6320.46 mg/L

Conclusions:

The water solubility for the test item Benzimidazole (CAS no. 57-17-2) was found to be 6320.46 mg/L at 30°C.

Executive summary:

The water solubility determination of test item Benzimidazole (CAS No. 51-17-2) was done as per the OECD guideline 105 and by spectrophotometric analytical method as per OECD 101. The solubility of Benzimidazole (CAS: 51-17-2 ) in water was determined to be 6320.46 mg/L at 30°C.

Hence, on the basis of experimental value the Benzimidazole (CAS No. 51-17-2) is considered to be soluble in water.

Description of key information

The water solubility for the test item Benzimidazole (CAS no. 57-17-2) was found to be 6320.46 mg/L at 30°C.

Key value for chemical safety assessment

Water solubility:

6 320.46 mg/L

at the temperature of:

30 °C

Additional information

The water solubility determination of test item Benzimidazole (CAS No. 51-17-2) was done as per the OECD guideline 105 and by spectrophotometric analytical method as per OECD 101. The solubility of Benzimidazole (CAS: 51-17-2 ) in water was determined to be 6320.46 mg/L at 30°C.

Hence, on the basis of experimental value the Benzimidazole (CAS No. 51-17-2) is considered to be soluble in water.