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EC number: 268-544-3
CAS number: 68123-13-7
Eye irritation study was performed on New Zealand rabbits to evaluate the irritant nature of the test compound Basic Blue 99
A solution of 0.5% Basic Blue 99 (0.1 ml) was instilled into the conjunctinal sac of the left eye and the right eye received 0.1 ml of vehicle alone (saline). Eye irritation was read at 30 and 60 min and 1 and 2 days post instillation. The conjunctivae of the Basic Blue 99 treated eye were discolored, but no effects on the cornea or iris were observed in any animal. The study concluded that the mucous membrane injury threshold concentration for the rabbit eye is greater than 0.5% Basic Blue 99.
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