Registration Dossier

Ecotoxicological information

Short-term toxicity to aquatic invertebrates

Administrative data

Endpoint:
short-term toxicity to aquatic invertebrates
Type of information:
experimental study
Adequacy of study:
key study
Study period:
17 July - 6 September 2000
Reliability:
1 (reliable without restriction)
Rationale for reliability incl. deficiencies:
other: Guideline study (OECD 202) and in compliance with GLP.

Data source

Reference
Reference Type:
study report
Title:
Unnamed
Year:
2000
Report Date:
2000

Materials and methods

Test guidelineopen allclose all
Qualifier:
according to
Guideline:
OECD Guideline 202 (Daphnia sp. Acute Immobilisation Test)
Qualifier:
according to
Guideline:
EU Method C.2 (Acute Toxicity for Daphnia)
Qualifier:
according to
Guideline:
EPA OPPTS 850.1010 (Aquatic Invertebrate Acute Toxicity Test, Freshwater Daphnids)
GLP compliance:
yes (incl. certificate)

Test material

Reference
Name:
Unnamed
Type:
Constituent
Details on test material:
- Name of test material (as cited in study report): TH 1165 I; (4-Methoxyphenyl)-aceton
- Physical state: yellow liquid
- Analytical purity: 99.01 %
- Purity test date: 6. March 2000
- Lot/batch No.: 14
- Expiration date of the lot/batch: can be requested from the sponsor
- Stability under test conditions: can be requested from the sponsor
- Storage condition of test material: in original container, at room temperature, in the dark

Test solutions

Vehicle:
no
Details on test solutions:
The test medium with the test item concentration of nominal 100 mg/L was prepared by dissolving 50 mg test item homogeneously in 500 ml test water by intense stirring for 15 minutes and short ultrasonic treatment for 2 minutes. The test medium was freshly prepared just before the start of the test (= introduction of Daphnia).

Test organisms

Test organisms (species):
Daphnia magna
Details on test organisms:
TEST ORGANISM
- Common name: Daphnia magna (Straus)
- Source: supplied 1997 by the Umweltbundesamt, Institut für Wasser-, Boden- und Lufthygiene, Berlin, Germany
- Age at study initiation (mean and range, SD): 7.5 - 23.5 hours
- Sex: female
- Method of breeding: The Daphnia were bred in the laboratories of IBACON under similar temperature and light conditions as in the test, and in reconstituted water of a similar quality regarding pH, components of the main ions and total hardness as the test water used in the test. The test organisms were not first brood progeny.

ACCLIMATION
- Acclimation period: for 7.5 hours under test conditions
- Acclimation conditions (same as test or not): yes, similar
- Type and amount of food: none

Study design

Test type:
static
Water media type:
freshwater
Limit test:
yes
Total exposure duration:
48 h

Test conditions

Hardness:
Water hardness was reported as 250 mg/L CaCO3
Test temperature:
21°C
pH:
8.0
Dissolved oxygen:
The dissolved oxygen concentration at the end of the test was >60% of the air saturation value at the temperature used.
Salinity:
Not applicalbe
Nominal and measured concentrations:
nominal concentration: 100 mg/l
Details on test conditions:
TEST SYSTEM
The Test vessels were glass beakers of 50 ml test medium. Each concentration had 2 replicate vessels and each vessel contain 10 test organisms. The age of the test organisms were < 24 hours and they were not fed during the test. Vessels were kept at 21.0°C .
Reference substance (positive control):
no

Results and discussion

Effect concentrations
Duration:
48 h
Dose descriptor:
EC50
Effect conc.:
> 100 mg/L
Nominal / measured:
nominal
Basis for effect:
mobility

Applicant's summary and conclusion

Validity criteria fulfilled:
yes
Conclusions:
A 48 hour EC50 value higher than 100 mg test item/L was determined in a reliable study conducted according to an appropriate OECD test guideline.