Dibenzo[d,f][1,3,2]dioxaphosphepin, 6,6'-[[3,3',5,5'-tetrakis(1,1-dimethylethyl)[1,1'-biphenyl]-2,2'-diyl]bis(oxy)]bis-

Short-term toxicity to aquatic invertebrates

Administrative data

Endpoint:

short-term toxicity to aquatic invertebrates

Type of information:

experimental study

Adequacy of study:

key study

Study period:

2012-11-26 to 2012-11-29 (experimental phase)

Reliability:

1 (reliable without restriction)

Rationale for reliability incl. deficiencies:

other: GLP guideline study with analytical monitoring

Data source

Reference

Reference Type:

study report

Title:

Unnamed

Year:

2013

Report date:

2013

Materials and methods

Test guideline

Qualifier:

according to guideline

Guideline:

OECD Guideline 202 (Daphnia sp. Acute Immobilisation Test)

GLP compliance:

yes (incl. QA statement)

Test material

Test material information

Constituent 1

Test material form:

other: solid

Details on test material:

- Batch Number: 0005473663
- Purity: > 98 %
- Appearance/colour: solid/white
- Expiry date: 01 February 2013
- Storage: At ambient temperature

Sampling and analysis

Analytical monitoring:

yes

Details on sampling:

Method: Analytical verification of application of the suspension was performed before filtration. A separate weighing of 10 mg/100 mL was prepared and stirred for 24 hours. After the stirring period the complete suspension was extracted. The analysis of samples from the separate, unfiltrated suspension of the test item was performed in order to demonstrate correct application of the test item. It was not the intention to quantify the water-soluble fraction of the test item since the water solubility lies below the LOQ of 1.0 mg/L.

Test solutions

Vehicle:

no

Details on test solutions:

PREPARATION AND APPLICATION OF TEST SOLUTION
- Method: 50 mg of the test substance was weighed on a weighing scoop and transferred to a 500 mL volumetric flask. Test medium was added up to the bench mark. The solution was stirred for 24 hours, afterwards insoluble particles of the test item were separated from the liquid phase by filtration through a filter with 0.45 μm mean pore size (according to OECD SERIES ON TESTING AND ASSESSMENT No. 23). The resulting filtrate was used as limit test concentration.

Test organisms

Test organisms (species):

Daphnia magna

Details on test organisms:

TEST ORGANISM
- Common name: Water flea
- Strain: Clone V
- Source: Federal Environmental Agency in Berlin/Germany
- Age at study initiation: 6 - 24 hours
- Feeding during test: none

Study design

Test type:

static

Water media type:

freshwater

Limit test:

yes

Total exposure duration:

48 h

Test conditions

Hardness:

13°dH (232 mg/L CaCO3)

Test temperature:

19.5 – 21.0 °C

pH:

7.44 – 8.47

Dissolved oxygen:

83 - 99 % of air saturation

Nominal and measured concentrations:

Nominal concentrations: 100 mg/L (loading rate)
Measured concentrations: 103 % of the application rate of the test item was analytically recovered from the unfiltered suspension (100 mg/L loading rate), thus confirming correct dosage of the insoluble test item. The toxicological endpoints were evaluated using nominal concentrations.

Details on test conditions:

TEST SYSTEM
- Test vessel: four 100 mL glass beakers per concentration
- Fill volume: 50 mL per test vessel
- Aeration: none
- No. of organisms per vessel: 5
- No. of vessels per concentration: 4
- No. of vessels per control (replicates): 4

TEST MEDIUM / WATER PARAMETERS
- Source/preparation of dilution water: According to guideline
- Intervals of water quality measurement: daily

OTHER TEST CONDITIONS
- Photoperiod: 16 hours of illumination and 8 hours of darkness

EFFECT PARAMETERS MEASURED: Mobility

Reference substance (positive control):

yes

Remarks:

Potassium dichromate

Results and discussion

Effect concentrations

Effect concentrations 1

Duration:

48 h

Dose descriptor:

EC50

Effect conc.:

> 100 mg/L

Nominal / measured:

nominal

Conc. based on:

test mat.

Basis for effect:

mobility

Remarks on result:

other: loading rate

Effect concentrations 2

Duration:

48 h

Dose descriptor:

NOEC

Effect conc.:

>= 100 mg/L

Nominal / measured:

nominal

Conc. based on:

test mat.

Basis for effect:

mobility

Remarks on result:

other: loading rate

Details on results:

- Mortality of control: none

Results with reference substance (positive control):

The EC50 (48 h) was determined to be between 1.0 and 2.0 mg/L

Applicant's summary and conclusion

Validity criteria fulfilled:

yes