Use of this information is subject to copyright laws and may require the permission of the owner of the information, as described in the ECHA Legal Notice.
EC number: 202-993-8
CAS number: 101-97-3
Absorbance570 nmTissue 1 *
Absorbance570 nmTissue 2 *
Absorbance570 nmTissue 3 *
MeanAbsorbanceof 3 Tissues
Rel. Absorbance[%] Tissue1, 2 +3 **
Mean Rel.Absorbance[% ofNegativeControl] ***
* Mean of three replicate wells after blank
** relative absorbance per tissue (rounded
values): 100 x (absorbance tissue)/mean absorbance negative control)
*** relative absorbance per treatment group
(rounded values): 100 x (mean absorbance test/positive control)/(mean
absorbance negative control)
The optical pre-experiment (colour
interference pre-experiment) to investigate the test item’s colour
change potential in water did not led to a change in colour and the
colour of the test item was not intensive => additional test with viable
tissues without MTT addition was not necessary
Optical evaluation of the MTT-reducing
capacity of the test item after 1 hour incubation with MTT-reagent did
not show blue colour => additional test with freeze-killed tissues
without MTT addition was not necessary
The mean relative absorbance value of the
test item, corresponding to the cell viability, was not reduced (89.1%;
threshold for irritancy: ≤ 50%), consequently the test item was not
irritant to skin.
This in vitro study was performed to assess
the irritation potential of the test item by means of the Human Skin
Model Test. The test item did not prove to be a MTT reducer (test for
direct MTT reduction), and it did not change colour when mixed with
deionised water (test for colour interference). Also its intrinsic
colour was not intensive. Consequently, additional tests with
freeze-killed or viable tissues were not necessary. Each three tissues
of the human skin model EpiDerm™ were treated with the test item, the
negative or the positive control for 60 minutes. Each 30 μL of the test
item, of the negative control (DPBS), and of the positive control (5%
SLS) were applied to each tissue and spread to match the surface of the
After treatment with the negative control
the absorbance values were well in the required range of the
acceptability criterion of mean OD ≥ 0.8 and ≤ 2.8 for the 60 minutes
treatment interval, thus assuring the quality of the tissues. Treatment
with the positive control induced a sufficient decrease in the relative
absorbance as compared to the negative control for the 60 minutes
treatment interval, and thus assuring the validity of the test system.
After treatment with the test item the mean relative absorbance value
decreased to 89.1% compared to the relative absorbance value of the
negative control. This value is above the threshold for irritancy of ≤
50%. Therefore, the test item is not considered to possess an irritant
In conclusion, it can be stated that in this
study and under the experimental conditions reported, the tes titem is
not irritant to skin.
Information on Registered Substances comes from registration dossiers which have been assigned a registration number. The assignment of a registration number does however not guarantee that the information in the dossier is correct or that the dossier is compliant with Regulation (EC) No 1907/2006 (the REACH Regulation). This information has not been reviewed or verified by the Agency or any other authority. The content is subject to change without prior notice.Reproduction or further distribution of this information may be subject to copyright protection. Use of the information without obtaining the permission from the owner(s) of the respective information might violate the rights of the owner.
På den här webbplatsen används kakor. Syftet är att optimera din upplevelse av den.
Welcome to the ECHA website. This site is not fully supported in Internet Explorer 7 (and earlier versions). Please upgrade your Internet Explorer to a newer version.
Do not show this message again