3-chloropropionyl chloride

Short-term toxicity to fish

Administrative data

Endpoint:

short-term toxicity to fish

Type of information:

experimental study

Adequacy of study:

key study

Reliability:

1 (reliable without restriction)

Rationale for reliability incl. deficiencies:

guideline study

Data source

Reference

Reference Type:

study report

Title:

Unnamed

Year:

1998

Report date:

1998

Materials and methods

Test guideline

Test guideline 1

Qualifier:

according to guideline

Guideline:

OECD Guideline 203 (Fish, Acute Toxicity Test)

Test guideline 2

Qualifier:

according to guideline

Guideline:

EU Method C.1 (Acute Toxicity for Fish)

GLP compliance:

yes

Test material

Test material information

Constituent 1

Reference substance name:

3-chloropropionyl chloride

EC Number:

210-890-4

EC Name:

3-chloropropionyl chloride

Cas Number:

625-36-5

Molecular formula:

C3H4Cl2O

IUPAC Name:

3-chloropropanoyl chloride

Details on test material:

- Name of test material (as cited in study report): 3-Chlorpropionsaeurechlorid
- Physical state: liquid, clear, brown
- Storage condition of test material: room temperature, tightly closed bottle

Specific details on test material used for the study:

- Name of test material (as cited in study report): 3-Chlorpropionsaeurechlorid
- Physical state: liquid, clear, brown
- Storage condition of test material: room temperature, tightly closed bottle

Sampling and analysis

Analytical monitoring:

yes

Test solutions

Vehicle:

no

Details on test solutions:

PREPARATION AND APPLICATION OF TEST SOLUTION
- Method: Separately for each concentration 0.2151, 0.4641, 1.01, 2.15, 4.64 and 4.64 (pH-adjustment) g the test substance were added to the test water using an ultra-turrax stirrer. From the beginning of preparing the test solutions until termination of placing the fish into the aquaria 35 minutes were needed.

Test organisms

Test organisms (species):

Danio rerio (previous name: Brachydanio rerio)

Details on test organisms:

TEST ORGANISM
- Common name: Zebra fish
- Source: Charles River Aquatics, Someren, Netherlands
- Age at study initiation: ca. 4 months
- Length at study initiation (mean, range): 2.68 (2.4 - 3.0) cm
- Weight at study initiation (mean, range): 0.232 (0.16 - 0.36) g
- Feeding during test: 1 day before and during exposure

HOUSING
- The fish were kept in a flow-through tank in tap water, not chlorinated, passed through a charcoal filter and aerated with oil-free air.
- Duration of housing: About 2 months
- Mortality during the last 14 days: 0.19%
- Diet: "TETRA MIN" standard feed for aquarium fish TETRA - WERKE, MELLE, Germany, ad libitum; and on workdays generally additionally live artemia
- Adaptation: Test conditions corresponded to housing conditions

Study design

Test type:

static

Water media type:

freshwater

Limit test:

no

Total exposure duration:

96 h

Test conditions

Hardness:

ca. 2.5 mmol/L

Test temperature:

22 °C

pH:

Test concentrations without pH-adjustment (0 - 464 mg/L): 3.9 - 8.5
Test concentration with pH-adjustment (464 mg/L): 7.7

Dissolved oxygen:

6.6 - 8.6 mg/L

Nominal and measured concentrations:

- Without pH-adjustment:
- Nominal concentration: control, 21.5, 46.4, 100, 215, 464 mg/L
- Recovery rates after 1 hour (%): < 10, 102.3, 97.0, 102.0, 103.3, 97.8
- Recovery rates after 96 hours (%): < 10, 93.0, 97.0, 99.0, 94.9, 101.5
- With pH-adjustment:
- Nominal concentration: 464 mg/L
- Recovery rates after 1 hour (%): 100.0
- Recovery rates after 96 hours (%): 95.7

Details on test conditions:

TEST SYSTEM
- Test vessel:
- Type: Aquaria
- Material, size, fill volume: glass, 30x22x24 cm, 10 L
- Aeration: slight
- No. of organisms per vessel: 10
- No. of vessels per concentration (replicates): 1
- No. of vessels per control (replicates): 1
- Biomass loading rate: 0.2 g/L
- Intervals of water quality measurement: 1, 24, 48, 72, 96 h

TEST MEDIUM / WATER PARAMETERS
- Source/preparation of dilution water: Municipal water of the city of Frankenthal, not chlorinated and passed through a charcoal filter, aerated
- Ca/Mg ratio: 9:1
- Culture medium different from test medium: no

OTHER TEST CONDITIONS
- Adjustment of pH: yes, the pH of the highest concentration (464 mg/L) was adjusted
- Photoperiod: 16 h light, 8 h darkness

EFFECT PARAMETERS MEASURED: mortality and symptoms after 1, 4, 24, 48, 72, 96 h

TEST CONCENTRATIONS
- Spacing factor for test concentrations: 2.15
- Range finding study: yes (LC50 after 96 h: > 100 mg/L)

Results and discussion

Effect concentrations

Effect concentrations 1

Key result

Duration:

96 h

Dose descriptor:

LC50

Effect conc.:

> 100 - < 215 mg/L

Nominal / measured:

nominal

Conc. based on:

test mat.

Remarks:

analytically verified

Basis for effect:

mortality (fish)

Effect concentrations 2

Duration:

96 h

Dose descriptor:

LC50

Effect conc.:

> 99 - < 204 mg/L

Nominal / measured:

meas. (not specified)

Conc. based on:

test mat.

Basis for effect:

mortality (fish)

Details on results:

- Behavioural abnormalities: apathy, narcotic-like state
- Mortality of control: none

Reported statistics and error estimates:

Finney, D.J., Probit Analysis, Cambr. Univ. Press, 3rd ed., 1971; certain aspects of this method have been modified.

Any other information on results incl. tables

Sublethal observations / clinical signs:

Cumulative mortalities:

Concentration (mg/L)	No. of fish at test start	Dead fish after
		1 h	4 h	24 h	48 h	72 h	96 h
0 (control)	10	0	0	0	0	0	0
21.5	10	0	0	0	0	0	0
46.4	10	0	0	0	0	0	0
100	10	0	0	0	0	0	0
215	10	0	0	5	8	8	8
464	10	10	10	10	10	10	10
464*	10	0	10	10	10	10	10

* pH-adjusted