2-imino-1-methylimidazolidin-4-one

Short-term toxicity to aquatic invertebrates

Link to relevant study record(s)

Reference

Endpoint:

short-term toxicity to aquatic invertebrates

Type of information:

experimental study

Adequacy of study:

key study

Study period:

24.09.2002 - 11.10.2002

Reliability:

1 (reliable without restriction)

Rationale for reliability incl. deficiencies:

guideline study

Qualifier:

according to guideline

Guideline:

EU Method C.2 (Acute Toxicity for Daphnia)

GLP compliance:

not specified

Analytical monitoring:

not specified

Details on sampling:

no data

Vehicle:

no

Details on test solutions:

A stock solution of the test substance with a concentration of 1000 mg/L was prepared in reconstituted water. Further dilutions of the test substance were made in reconstituted water (according to Guideline ISO 6341).

Test organisms (species):

Daphnia magna

Details on test organisms:

TEST ORGANISM
- Common name: Daphnia magna STRAUSS
- Source: Landesamt für Wasserwirtschaft München
- Method of breeding: Subparts of the culture are cultivated in 2 L beakers in approx. 1,7 L hatching medium. Every 3-4 days the cultures are transfered into new medium and the young animals are separated from the older ones by sieving. The daphnids are fed every day with an aliquot of a culture of single cell green algae.
- Feeding during test: none

ACCLIMATION
- Acclimation period: not necessary because animals were hatched in the laboratory (under the same conditions as in the test)

Test type:

static

Water media type:

freshwater

Limit test:

no

Total exposure duration:

48 h

Post exposure observation period:

no data

Hardness:

no data

Test temperature:

Temperature was stable during the test period (20 °C)

pH:

7.4 - 7.9

Dissolved oxygen:

6.8 - 8.4

Salinity:

no data

Nominal and measured concentrations:

Preliminary test: 0, 1, 2, 10, 100, 33, 500 mg/L
Main Test: 0, 250, 333, 500, 1000 mg/L

Details on test conditions:

See "any other infomraion on material and methods"

Reference substance (positive control):

yes

Remarks:

potassium dichromate

Key result

Duration:

48 h

Dose descriptor:

EC50

Effect conc.:

> 1 000 mg/L

Conc. based on:

test mat.

Basis for effect:

mortality

Details on results:

see "any other information on results"

Results with reference substance (positive control):

To check the correct performance of the test procedure and the sensitivity of the test animals a reference test was periodically performed with potassium dichromate. The last reference test was performed in June 2002. The EC50 value (EC50 24 h; 1,4 mg/L) was within the limit of 0.6 - 1,7 mg/L indicated in ISO 6341.

Reported statistics and error estimates:

no data

Validity criteria fulfilled:

yes

Conclusions:

The results show that at a test substance concentration of 1000 mg/L no daphnids were immobilized. Therefore the EC50 of Creatinine is > 1000 mg/L.

Executive summary:

The acute toxicity of Creatinine on Daphnia magna was determined in a 48 hours static test following the pattern of EEC Commission Directive 92/69, C.2 "Acute Toxicity for Daphnia magna". Animals of Daphnia magna, not more than 24 hours old, were exposed to different concentrations of the test substance in reconstituted water according to ISO 6341. A negative control group was exposed to reconstituted water only. In the test 20 daphnids, divided into 4 replicates (5 daphnia each) were used for each concentration and for the control group. The results show that at a test substance concentration of 1000 mg/L no daphnids were immobilized. Therefore the EC50 of Creatinine is > 1000 mg/L.

Description of key information

The results show that at a test substance concentration of 1000 mg/L no daphnids were immobilized.

Therefore the EC50 of Creatinine is > 1000 mg/L.

Additional information

The acute toxicity of Creatinine on Daphnia magna was determined in a 48 hours static test following the pattern of EEC Commission Directive 92/69, C.2 "Acute Toxicity for Daphnia magna". Animals of Daphnia magna, not more than 24 hours old, were exposed to different concentrations of the test substance in reconstituted water according to ISO 6341. A negative control group was exposed to reconstituted water only. In the test 20 daphnids, divided into 4 replicates (5 daphnia each) were used for each concentration and for the control group. The results show that at a test substance concentration of 1000 mg/L no daphnids were immobilized. Therefore the EC50 of Creatinine is > 1000 mg/L.