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Ecotoxicological information

Short-term toxicity to aquatic invertebrates

Administrative data

Endpoint:
short-term toxicity to aquatic invertebrates
Type of information:
experimental study
Adequacy of study:
key study
Reliability:
1 (reliable without restriction)
Rationale for reliability incl. deficiencies:
other: GLP guideline study

Data source

Reference
Reference Type:
study report
Title:
Unnamed
Year:
2009
Report Date:
2009

Materials and methods

Test guideline
Qualifier:
according to
Guideline:
OECD Guideline 202 (Daphnia sp. Acute Immobilisation Test)
GLP compliance:
yes (incl. certificate)

Test material

Reference
Name:
Unnamed
Type:
Constituent
Details on test material:
- Name of test material (as cited in study report): Gelb LD 6259
- Lot/batch No.: Lot 20980
- Purity test date: 87.6 - 89.1 area %

Sampling and analysis

Analytical monitoring:
yes

Test solutions

Details on test solutions:
PREPARATION AND APPLICATION OF TEST SOLUTION (especially for difficult test substances)
- Method: 239.94 mg test substance were dissolved in 2 l test medium and stirred for 10 minutes at 20 +/- 2 °C

Test organisms

Test organisms (species):
Daphnia magna
Details on test organisms:
TEST ORGANISM
- Source: originally obtained from the Institute National de Recherche Chimique Appliquée, France and cultured since 1978 at the test facility
- Age at study initiation (mean and range, SD): < 24 h

Study design

Test type:
static
Water media type:
freshwater
Limit test:
yes
Total exposure duration:
48 h

Test conditions

Hardness:
2.2 - 3.2 mmol/l
Test temperature:
19.6 - 20.5 °C
pH:
7.9 - 8.1
Dissolved oxygen:
8.6 - 8.8 mg/l
Nominal and measured concentrations:
Nominal test concentration: 120 mg/l
The analytically determined concentration was within 20 % of the nominal concentration.
Analytically determined concentrations:
0 hours: 119.7 mg/l
48 hours: 124.5 mg/l
Details on test conditions:
TEST SYSTEM
- Test vessel: glass beakers (250 ml)
- Fill volume: 200 ml
- No. of organisms per vessel: 5
- No. of vessels per concentration (replicates): 4
- No. of vessels per control (replicates): 4
- Biomass loading rate: 0.025 animals/ml

TEST MEDIUM / WATER PARAMETERS
- Source/preparation of dilution water: synthetic fresh water (M 4 medium )
- Ca/mg ratio: 4:1
- Conductivity: 550 - 650 µS/cm


OTHER TEST CONDITIONS
- Photoperiod: 16:8 h day-night regime
- Light intensity: 20 - 850 lux at a wave length of 400 - 750 nm


EFFECT PARAMETERS MEASURED (with observation intervals if applicable) :
Mobility after 0, 24 and 48 hours

TEST CONCENTRATIONS
- Results used to determine the conditions for the definitive study: EC50 (48 h) > 100 mg/l
Reference substance (positive control):
yes
Remarks:
Potassium dichromate

Results and discussion

Effect concentrationsopen allclose all
Duration:
48 h
Dose descriptor:
EC0
Effect conc.:
>= 120 mg/L
Nominal / measured:
nominal
Conc. based on:
test mat.
Basis for effect:
mobility
Duration:
48 h
Dose descriptor:
EC50
Effect conc.:
> 120 mg/L
Nominal / measured:
nominal
Conc. based on:
test mat.
Basis for effect:
mobility
Duration:
48 h
Dose descriptor:
EC100
Effect conc.:
> 120 mg/L
Nominal / measured:
nominal
Conc. based on:
test mat.
Basis for effect:
mobility
Results with reference substance (positive control):
The EC50(48h) of the reference substance potassium dichromate was 1.13 mg/L.
(Date of the last control experiment: 12 Feb 2009, project-no. 50E0063/043039)

Applicant's summary and conclusion

Validity criteria fulfilled:
yes
Conclusions:
In conclusion the 48h EC50 for Gelb LD 6259 with Daphnia magna were >120 mg/L based on the nominal concentration of the test substance and >122 mg/L based on the mean of analytically determined concentrations.