Registration Dossier

Environmental fate & pathways

Biodegradation in water: screening tests

Administrative data

Endpoint:
biodegradation in water: ready biodegradability
Type of information:
experimental study
Adequacy of study:
key study
Reliability:
1 (reliable without restriction)
Rationale for reliability incl. deficiencies:
other: GLP guideline study

Data source

Reference
Reference Type:
study report
Title:
Unnamed
Year:
2009
Report Date:
2009

Materials and methods

Test guideline
Qualifier:
according to
Guideline:
OECD Guideline 301 B (Ready Biodegradability: CO2 Evolution Test)
GLP compliance:
yes (incl. certificate)

Test material

Reference
Name:
Unnamed
Type:
Constituent
Details on test material:
- Name of test material (as cited in study report): Gelb LD 6259
- Lot/batch No.: 0817VP01
- Analytical purity: The IR spectrum shows the expected bands; the 1H and 13C NMR spectra show expected signals for the current structure (BASF report No. 08L00346
- Physical state: solid

Study design

Oxygen conditions:
aerobic
Inoculum or test system:
activated sludge, domestic, non-adapted
Details on inoculum:
- Source of inoculum/activated sludge (e.g. location, sampling depth, contamination history, procedure): municipal activated sludge taken from the waste water treatment plant of Mannheim (Baden-Wuertemberg, Germany).
- Preparation of inoculum for exposure: the inoculum was sieven (mesh size 1 mm) and pre-aerated
- Concentration of sludge: 30 mg/l
Initial test substance concentrationopen allclose all
Initial conc.:
86 mg/L
Based on:
test mat.
Initial conc.:
20 mg/L
Based on:
DOC
Parameter followed for biodegradation estimation
Parameter followed for biodegradation estimation:
CO2 evolution
Details on study design:
TEST CONDITIONS
- Test temperature: 22 +/- 2 °C


TEST SYSTEM
- Number of culture flasks/concentration: 2


CONTROL AND BLANK SYSTEM
- Inoculum blank: yes
- Abiotic sterile control: yes
- Toxicity control: yes


Reference substance
Reference substance:
aniline

Results and discussion

% Degradation
Parameter:
% degradation (CO2 evolution)
Value:
< 10
Sampling time:
28 d

Applicant's summary and conclusion

Validity criteria fulfilled:
yes
Interpretation of results:
other: poorly biodegradable
Conclusions:
The test substance was poorly biodegradable and according to OECD criteria "not readily biodegradable " in this test.