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Ecotoxicological information

Short-term toxicity to aquatic invertebrates

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Description of key information

The test substance showed a low acute toxicity to Daphnia magna with an EC50 of 748 mg/L calculated as the geometric mean of the test concentration with and without effect.

Key value for chemical safety assessment

EC50/LC50 for freshwater invertebrates:
748 mg/L

Additional information

Reactive Black 5-OH, the hydrolysis product of Reactive Black 5, a structural analogue and impurity of the test compound, was tested for acute toxic effects on Daphnia magna according to OECD 202 at concentrations of 2 to 128 mg/L over an exposure time of 48 hours. No adverse effects were observed during this study. The EC0 and EC50 values are above the highest test concentration of 128 mg/L, tested.

Reactive Black 5, a structural analogue and impurity of the test compound, was tested for acute toxic effects on Daphnia magna according to OECD 202 at concentrations of 320, 560 and 1000 mg/L over an exposure time of 48 hours in a semi-static test under slightly acidic test conditions (pH 6) to avoid abiotic degradation of the test substance. No adverse effects were observed during this study at 320 and 560 mg/L. At 1000 mg/L all daphnids were immobile after 48 hours. The EC0 was 560 mg/L, the EC50 was calculated (as the geometric mean between 560 and 1000 mg/) to be 748 mg/L, the EC100 was 1000 mg/L.