Registration Dossier
Registration Dossier
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EC number: 201-877-4 | CAS number: 89-04-3
- Life Cycle description
- Uses advised against
- Endpoint summary
- Appearance / physical state / colour
- Melting point / freezing point
- Boiling point
- Density
- Particle size distribution (Granulometry)
- Vapour pressure
- Partition coefficient
- Water solubility
- Solubility in organic solvents / fat solubility
- Surface tension
- Flash point
- Auto flammability
- Flammability
- Explosiveness
- Oxidising properties
- Oxidation reduction potential
- Stability in organic solvents and identity of relevant degradation products
- Storage stability and reactivity towards container material
- Stability: thermal, sunlight, metals
- pH
- Dissociation constant
- Viscosity
- Additional physico-chemical information
- Additional physico-chemical properties of nanomaterials
- Nanomaterial agglomeration / aggregation
- Nanomaterial crystalline phase
- Nanomaterial crystallite and grain size
- Nanomaterial aspect ratio / shape
- Nanomaterial specific surface area
- Nanomaterial Zeta potential
- Nanomaterial surface chemistry
- Nanomaterial dustiness
- Nanomaterial porosity
- Nanomaterial pour density
- Nanomaterial photocatalytic activity
- Nanomaterial radical formation potential
- Nanomaterial catalytic activity
- Endpoint summary
- Stability
- Biodegradation
- Bioaccumulation
- Transport and distribution
- Environmental data
- Additional information on environmental fate and behaviour
- Ecotoxicological Summary
- Aquatic toxicity
- Endpoint summary
- Short-term toxicity to fish
- Long-term toxicity to fish
- Short-term toxicity to aquatic invertebrates
- Long-term toxicity to aquatic invertebrates
- Toxicity to aquatic algae and cyanobacteria
- Toxicity to aquatic plants other than algae
- Toxicity to microorganisms
- Endocrine disrupter testing in aquatic vertebrates – in vivo
- Toxicity to other aquatic organisms
- Sediment toxicity
- Terrestrial toxicity
- Biological effects monitoring
- Biotransformation and kinetics
- Additional ecotoxological information
- Toxicological Summary
- Toxicokinetics, metabolism and distribution
- Acute Toxicity
- Irritation / corrosion
- Sensitisation
- Repeated dose toxicity
- Genetic toxicity
- Carcinogenicity
- Toxicity to reproduction
- Specific investigations
- Exposure related observations in humans
- Toxic effects on livestock and pets
- Additional toxicological data

Long-term toxicity to aquatic invertebrates
Administrative data
Link to relevant study record(s)
- Endpoint:
- long-term toxicity to aquatic invertebrates
- Type of information:
- experimental study
- Adequacy of study:
- key study
- Reliability:
- 1 (reliable without restriction)
- Rationale for reliability incl. deficiencies:
- other: GLP compliant study conducted according to internationally recognised test methods.
- Qualifier:
- according to guideline
- Guideline:
- OECD Guideline 211 (Daphnia magna Reproduction Test)
- Deviations:
- no
- GLP compliance:
- yes
- Specific details on test material used for the study:
- - Lot/batch No.: 001101TMO
- Analytical monitoring:
- yes
- Details on sampling:
- - Nominal concentrations: 0, 5.29, 9.53, 17.1, 30.9, 55.6 and 100.0 mg/L
- Sampling method: 0.5 - 5.0 L sampled on 4 occasions - immediately after preparing test solution (fresh medium on Day 0), and 2 days later before renewal (exhausted medium)
- Sample storage conditions before analysis: no data - Vehicle:
- yes
- Details on test solutions:
- PREPARATION AND APPLICATION OF TEST SOLUTION (especially for difficult test substances)
- Method: Test substance solution at 3000 or 5000 mg/L concentration (in medium with HCO-50 solubiliser) was diluted to the required nominal concentration using medium (with solubilizer HCO-50 at 100 mg/L in final test/control media).
- Eluate: Dilution media
- Controls: yes, control & dispersant control
- Chemical name of vehicle (organic solvent, emulsifier or dispersant): solubilizer HCO-50
- Concentration of vehicle in test medium (stock solution and final test solution(s) including control(s)): 1.0 wt .% dispersant, 100 mg/l
- Evidence of undissolved material (e.g. precipitate, surface film, etc): No - test solutions were clear, colourless and with no observed precipitate - Test organisms (species):
- Daphnia magna
- Details on test organisms:
- TEST ORGANISM
- Common name: Daphnia magna
- Justification for species other than prescribed by test guideline: N/A
- Source: NIES Japan
- Age of parental stock (mean and range, SD): within 24h of age
- Feeding during test, yes
- Food type: Chlorella vulgaris
- Amount: 0.1-0.2 mgC/day/individual
- Frequency: daily - Test type:
- semi-static
- Water media type:
- freshwater
- Limit test:
- no
- Total exposure duration:
- 21 d
- Post exposure observation period:
- None
- Hardness:
- 244-255 mg/L as CaCO3
- Test temperature:
- 19.1-20.2 °C
- pH:
- 7.1-7.8.
- Dissolved oxygen:
- 7.1-8.8 mg/L
- Salinity:
- N/A
- Nominal and measured concentrations:
- Nominal concentrations: 0, 5.29, 9.53, 17.1, 30.9, 55.6 and 100.0 mg/L
Measured concentrations: See Results table - Details on test conditions:
- TEST SYSTEM
- Test vessel: glass beaker
- Material, size, headspace, fill volume: 80 mL
- Aeration: No data
- Renewal rate of test solution (frequency/flow rate): 3 times/week
- No. of organisms per vessel: 10
- No. of vessels per concentration (replicates): 10
- No. of vessels per control (replicates): 10
- No. of vessels per vehicle control (replicates): 10
TEST MEDIUM / WATER PARAMETERS
- Source/preparation of dilution water: Elendt M4(OECD guideline No.211 Annex 2)
- Intervals of water quality measurement: 3 times, fresh and exhausted medium
OTHER TEST CONDITIONS
- Adjustment of pH: no
- Photoperiod: 16 hours light:8 hours dark
- Light intensity: =<1,200 lux
EFFECT PARAMETERS MEASURED (with observation intervals if applicable) : See results tables below
VEHICLE CONTROL PERFORMED: Yes
RANGE-FINDING STUDY: yes
- Test concentrations: for parent Daphnia (Acute immobilization test) was >100mg/L. Dispersant control was also tested
- Results used to determine the conditions for the definitive study: yes - Reference substance (positive control):
- no
- Duration:
- 21 d
- Dose descriptor:
- LC50
- Effect conc.:
- 99.3 mg/L
- Nominal / measured:
- meas. (TWA)
- Conc. based on:
- test mat.
- Basis for effect:
- mortality
- Remarks on result:
- other: 58.9 - 134.0
- Duration:
- 21 d
- Dose descriptor:
- EC50
- Effect conc.:
- 10.4 mg/L
- Nominal / measured:
- meas. (TWA)
- Conc. based on:
- test mat.
- Basis for effect:
- reproduction
- Remarks on result:
- other: 9.1 - 11.6
- Duration:
- 21 d
- Dose descriptor:
- LOEC
- Effect conc.:
- 7.18 mg/L
- Nominal / measured:
- meas. (TWA)
- Conc. based on:
- test mat.
- Basis for effect:
- reproduction
- Duration:
- 21 d
- Dose descriptor:
- NOEC
- Effect conc.:
- 4.04 mg/L
- Nominal / measured:
- meas. (TWA)
- Conc. based on:
- test mat.
- Basis for effect:
- reproduction
- Details on results:
- - Mortality of parent animals: See Results table below
- No. of offspring produced per day per female: See Results table below
- Number of males and females (parental) : 10 - Results with reference substance (positive control):
- N/A
- Reported statistics and error estimates:
- Eco-Statics (Version 1.1)
Method of calculating mean measured concentrations: Time-weighted mean. - Validity criteria fulfilled:
- yes
- Conclusions:
- Under the conditions that this test was conducted the following values were calculated:
NOEC (21-d, reproduction) : 4.04 mg/L,
LOEC (21-d, reproduction) : 7.18 mg/L,
EC50 (21-d, reproduction) : 10.4 mg/L
LC50 for parental Daphnia (21-d) : 99.3 mg/L - Executive summary:
Chronic toxicity to Daphnia has been investigated in a 21 -day reproduction test according to OECD test methods. Dissolution/dispersion agents were used to increase concentration of the substance in the test medium. The following values were calculated:
NOEC (21-day, reproduction): 4.04 mg/L;
LOEC (21 -day, reproduction): 7.18 mg/L;
EC50 (21 -day, reproduction): 10.4 mg/L;
LC50 parental (21 -day): 99.3 mg/L
- Endpoint:
- long-term toxicity to aquatic invertebrates
- Type of information:
- migrated information: read-across from supporting substance (structural analogue or surrogate)
- Adequacy of study:
- weight of evidence
- Study period:
- 1997-06-18 to 1997-07-09
- Reliability:
- 2 (reliable with restrictions)
- Rationale for reliability incl. deficiencies:
- other: Guideline study with acceptable restrictions. For read-across justification see Section 13.
- Qualifier:
- according to guideline
- Guideline:
- other: OECD Guideline 202 Part 2
- Deviations:
- yes
- Remarks:
- In the development of new EC-methods the test conditions deviate from the OECD-Guideline. The evaluation is adoated to the EC-guideline. No influence on the results is expected
- Qualifier:
- equivalent or similar to guideline
- Guideline:
- OECD Guideline 211 (Daphnia magna Reproduction Test)
- Deviations:
- yes
- Remarks:
- In the development of new EC-methods the test conditions deviate from the OECD-Guideline. The evaluation is adapted to the EC-guideline. No influence on the results is expected
- GLP compliance:
- yes
- Analytical monitoring:
- yes
- Details on sampling:
- For determination of stability of the test substance solution, a solution in deionized water was made and analyzed after 0 and 72 hours.
The stock solution for the test was deep-frozen at -18°C and reanalyzed every week during the test. - Vehicle:
- no
- Details on test solutions:
- As the test substance is poorly soluble in water, a 1 g/L mixture in M4 medium without NaHCO3 and FeSO4 containing Na2EDTA was prepared. This mixture was stirred for 18 hours and filtrated afterwards. The filtrate showed an initial content of 4.1 mg/L DOC. The filtrate was used as Stock solution.
For changes of test solutions every second or third day during the test the stock solution was deep-frozen at -18°C. One day before application the required amount of the frozen stock solution was thawed and after sampling for analytical purpose mixed with NaHCO3 and FeSO4 containing Na2EDTA. - Test organisms (species):
- Daphnia magna
- Details on test organisms:
- TEST ORGANISM
- Common name: water flea
- Strain: Daphnia magna Straus, clone 5
- Source: laboratory breed
- Age at study initiation: not older than 24 hours
- Method of breeding: The females were maintained in synthetic fresh water (M4 medium, Elendt, 1990). A reddish dark-brown coloration of the animals indicated that they were healthy.
- Feeding during test: daily with Scenedesmus subspicatus (days 0 to 6: 0.5x10E7 cells/daphnid; days 7 - 21: 1x10E7 cells/daphnid)
ACCLIMATION
- Acclimation period: Females were separated from the offspring 24 hours before the start of the test. The offspring produced overnight were transferred into test water and were used for the test.
- Type and amount of food: not mentioned
- Feeding frequency: not mentioned - Test type:
- semi-static
- Water media type:
- freshwater
- Limit test:
- yes
- Total exposure duration:
- 21 d
- Post exposure observation period:
- none
- Hardness:
- not mentioned
- Test temperature:
- 20 ± 1 °C
- pH:
- 7,6 - 8,2 over all test vessels. In one case a pH deviation of > 0.3 Units was found between the freshly prepared solution and the 2-3 day old solutions. As the reproduction rate was not impaired by this deviation, it was considered as not affecting the validity of this test.
- Dissolved oxygen:
- 92% to 107% of saturation
- Salinity:
- according to guideline (M4 medium, Elendt)
- Nominal and measured concentrations:
- Nominal: WSF
Measured: 4.5 mg/L (mean of measured concentrations,concentration of test solutions remained stable during 72 hours: deviations < 20%) - Details on test conditions:
- TEST SYSTEM
- Test vessel:
- Type (delete if not applicable): open
- Material, size, headspace, fill volume: Glass beakers, 100 mL filled with 60 mL test solution
- Aeration: not mentioned
- Renewal rate of test solution (frequency/flow rate): 3 times per week
- No. of organisms per vessel: 1
- No. of vessels per concentration (replicates): 10
- No. of vessels per control (replicates): 10
- Biomass loading rate: 1 organism/60 mL
TEST MEDIUM / WATER PARAMETERS
- Source/preparation of dilution water: Synthetic fresh water according to Elendt M4-Medium
- Culture medium different from test medium: no
- Intervals of water quality measurement: Day 0 (fresh samples), 2, 9, 12, 14, 16
OTHER TEST CONDITIONS
- Adjustment of pH: no
- Photoperiod: 16h light, 8h dark
- Light intensity: light: 600 lux, dark: 100 lux
EFFECT PARAMETERS MEASURED (with observation intervals if applicable) :
mortality and offspring on day 2, 5, 7, 9, 12, 14, 16, 19, 21
VEHICLE CONTROL PERFORMED: no - Reference substance (positive control):
- no
- Duration:
- 21 d
- Dose descriptor:
- NOEC
- Effect conc.:
- >= 4.5 mg/L
- Nominal / measured:
- meas. (arithm. mean)
- Conc. based on:
- dissolved
- Basis for effect:
- reproduction
- Reported statistics and error estimates:
- As the reproduction rate with test substance was higher than in the control samples, no statistic has been performed.
- Validity criteria fulfilled:
- yes
- Conclusions:
- The highest tested concentration at which no negative effects were observed on the reproduction rate or survival of the parent animals is:
NOEC >= 4.5 mg/L (highest concentration under test conditions)
The lowest tested concentration at which the first significant effects were observed on the reproduction rate or survival of the parent animals is:
LOEC > 4.5 mg/L (highest concentration under test conditions)
The concentrations were based on the product. - Executive summary:
The effect of 1,2,4-Benzenetricarboxylic acid, mixed decyl and octyl triesters on the immobilisation of Daphnia magna had been studied in accordance with OECD Guideline for Testing of Chemicals No. 202 part II.
Daphnia magna was exposed to 1,2,4-Benzenetricarboxylic acid, mixed decyl and octyl triesters in a semistatic reproduction test over 21 days. As the test substance is poorly soluble in water, a 1 g/L mixture with synthetic freshwater was prepared. This mixture was stirred for 18 hours and filtrated afterwards. For changes of test solutions during the test the stock solution was deep-frozen at -18°C. The analytical determination of the defrosted stock solutions used for the test showed that they were stable within the 3 days of exposure (deviation > 20%), therefore the mean of the measured values (4.5 mg/L) were used for evaluation.
The highest tested concentration at which no negative effects were observed on the reproduction rate or survival of parental animals is: NOEC >= 4.5 mg/L (highest test concentration under test conditions).
The lowest tested concentration at which the first significant effects were observed on the reproduction rate or survival of the parent animals is: LOEC > 4.5 mg/L (highest concentration under test conditions).
The concentrations were based on the product.
Referenceopen allclose all
Analysis by HPLC: Within 58.0-182.3% of the nominal concentration. Time-weighted mean of the measured concentrations of test chemical during the 21-day exposure were 4.04, 7.18, 14.9, 28.1, 62.3 and 134.0 mg/L.
Nominal |
Measured concentration (mg/L) |
|||||||
Concentration |
Day 0 |
Day 2 |
Day 2 |
Day 4 |
Day 6 |
Day 8 |
Day 13 |
Day 15 |
(mg/L) |
New |
Old |
New |
Old |
New |
Old |
New |
Old |
Control |
<0.006 |
<0.006 |
<0.006 |
<0.006 |
<0.006 |
<0.006 |
<0.006 |
<0.006 |
Dispersant control |
<0.006 |
<0.006 |
<0.006 |
<0.006 |
<0.006 |
<0.006 |
<0.006 |
<0.006 |
5.29 |
4.80 |
3.07 |
4.46 |
4.11 |
4.12 |
4.03 |
4.21 |
3.66 |
9.53 |
7.17 |
6.32 |
7.59 |
7.90 |
7.18 |
7.29 |
7.19 |
6.86 |
17.1 |
15.5 |
13.7 |
16.5 |
15.6 |
14.1 |
14.4 |
15.0 |
14.3 |
30.9 |
31.6 |
27.1 |
28.7 |
28.4 |
27.1 |
26.9 |
28.4 |
26.5 |
55.6 |
88.6 |
80.0 |
59.3 |
49.9 |
49.7 |
56.0 |
65.3 |
50.8 |
100.0 |
214.3 |
182.3 |
145.9 |
127.0 |
94.1 |
109.2 |
98.4 |
102.9 |
new: freshly prepared test solutions. old: test solution after 2-3 days exposure |
Measured |
Cumulative Number of Dead ParentalDaphniaon Day: |
||||||||||||||||||||
Concentration |
1 |
2 |
3 |
4 |
5 |
6 |
7 |
8 |
9 |
10 |
11 |
12 |
13 |
14 |
15 |
16 |
17 |
18 |
19 |
20 |
21 |
(mg/L) |
|
|
|
|
|
|
|
|
|
|
|
|
|
|
|
|
|
|
|
|
|
Control |
0 |
0 |
0 |
0 |
0 |
0 |
0 |
0 |
0 |
0 |
0 |
0 |
0 |
0 |
0 |
0 |
0 |
0 |
0 |
0 |
0 |
Dispersant control |
0 |
0 |
0 |
0 |
0 |
0 |
0 |
0 |
0 |
0 |
0 |
0 |
0 |
0 |
1 |
1 |
1 |
1 |
1 |
1 |
1 |
4.04 |
0 |
0 |
0 |
0 |
0 |
0 |
0 |
0 |
0 |
0 |
0 |
0 |
0 |
0 |
0 |
0 |
1 |
2 |
2 |
3 |
3 |
7.18 |
0 |
0 |
1 |
1 |
1 |
1 |
1 |
1 |
1 |
1 |
1 |
1 |
1 |
2 |
2 |
2 |
3 |
4 |
5 |
5 |
5 |
14.9 |
0 |
2 |
2 |
2 |
2 |
2 |
2 |
2 |
2 |
2 |
3 |
3 |
3 |
3 |
3 |
3 |
5 |
5 |
5 |
5 |
5 |
28.1 |
0 |
0 |
1 |
1 |
1 |
1 |
1 |
1 |
1 |
1 |
1 |
1 |
1 |
1 |
1 |
1 |
1 |
1 |
1 |
1 |
2 |
62.3 |
0 |
0 |
2 |
2 |
2 |
2 |
2 |
2 |
2 |
2 |
2 |
2 |
2 |
2 |
2 |
2 |
2 |
2 |
2 |
2 |
2 |
134.0 |
0 |
2 |
2 |
2 |
3 |
5 |
5 |
5 |
6 |
6 |
7 |
7 |
7 |
7 |
7 |
7 |
7 |
7 |
7 |
7 |
7 |
Measured |
Mean Cumulative Number of Juveniles produced/adult on Day: |
||||||||||||||||||||
Concentration |
1 |
2 |
3 |
4 |
5 |
6 |
7 |
8 |
9 |
10 |
11 |
12 |
13 |
14 |
15 |
16 |
17 |
18 |
19 |
20 |
21 |
(mg/L) |
|
|
|
|
|
|
|
|
|
|
|
|
|
|
|
|
|
|
|
|
|
Control |
0.0 |
0.0 |
0.0 |
0.0 |
0.0 |
0.0 |
0.0 |
0.0 |
18.2 |
19.8 |
19.9 |
68.3 |
71.6 |
71.6 |
106.5 |
110.3 |
110.3 |
153.7 |
161.8 |
161.8 |
197.7 |
Dispersant control |
0.0 |
0.0 |
0.0 |
0.0 |
0.0 |
0.0 |
0.0 |
0.0 |
1.2 |
2.6 |
14.7 |
18.9 |
36.8 |
64.7 |
78.2 |
88.9 |
107.9 |
120.1 |
123.0 |
150.0 |
162.8 |
4.04 |
0.0 |
0.0 |
0.0 |
0.0 |
0.0 |
0.0 |
0.0 |
0.0 |
2.0 |
2.9 |
5.1 |
16.4 |
36.0 |
43.6 |
59.9 |
84.9 |
90.7 |
105.9 |
115.6 |
122.9 |
135.4 |
7.18 |
0.0 |
0.0 |
0.0 |
0.0 |
0.0 |
0.0 |
0.0 |
0.0 |
0.0 |
0.0 |
0.2 |
0.2 |
31.4 |
34.6 |
34.6 |
51.0 |
65.2 |
65.2 |
70.6 |
93.0 |
93.0 |
14.9 |
0.0 |
0.0 |
0.0 |
0.0 |
0.0 |
0.0 |
0.0 |
0.0 |
0.0 |
1.2 |
1.2 |
1.2 |
12.4 |
12.4 |
12.4 |
30.8 |
36.0 |
36.0 |
38.6 |
51.4 |
51.4 |
28.1 |
0.0 |
0.0 |
0.0 |
0.0 |
0.0 |
0.0 |
0.0 |
0.0 |
0.0 |
0.0 |
1.4 |
3.0 |
4.6 |
9.4 |
10.3 |
10.3 |
25.5 |
28.6 |
28.6 |
39.0 |
44.1 |
62.3 |
0.0 |
0.0 |
0.0 |
0.0 |
0.0 |
0.0 |
0.0 |
0.0 |
0.0 |
0.0 |
0.0 |
0.0 |
0.0 |
0.3 |
1.8 |
1.8 |
3.9 |
5.1 |
5.1 |
9.6 |
12.9 |
134.0 |
0.0 |
0.0 |
0.0 |
0.0 |
0.0 |
0.0 |
0.0 |
0.0 |
0.0 |
0.0 |
0.0 |
0.0 |
0.0 |
0.0 |
0.0 |
0.0 |
0.0 |
0.0 |
0.0 |
0.0 |
0.0 |
Time (days) to first brood production |
||||||||
Vessel Number |
Measured concentration (mg/L) |
|||||||
Control |
Dispersion control |
4.04 |
7.18 |
14.9 |
28.1 |
62.3 |
134.0 |
|
1 |
10 |
13 |
9 |
- |
- |
- |
20 |
>21 |
2 |
9 |
13 |
- |
13 |
13 |
13 |
14 |
- |
3 |
9 |
10 |
10 |
- |
- |
11 |
20 |
>21 |
4 |
9 |
11 |
- |
- |
- |
- |
- |
- |
5 |
9 |
11 |
13 |
- |
17 |
12 |
- |
>21 |
6 |
9 |
9 |
12 |
13 |
- |
12 |
20 |
- |
7 |
9 |
- |
11 |
11 |
10 |
12 |
15 |
- |
8 |
9 |
11 |
- |
13 |
- |
11 |
15 |
- |
9 |
9 |
11 |
10 |
13 |
10 |
11 |
17 |
- |
10 |
9 |
9 |
10 |
- |
13 |
11 |
15 |
- |
Mean |
9.1 |
10.9 |
10.7 |
12.6 |
12.6 |
11.6 |
17.0 |
>21 |
Table #1: Number of living parent animals
Conc. [mg/L] | Parallel | 2d | 5d | 7d | 9d | 12d | 14d | 16d | 19d | 21d | Day 21 |
control |
1 | 1 | 1 | 1 | 1 | 1 | 1 | 1 | 1 | 1 | Total parent animals 9 |
2 | 1 | 1 | 1 | 1 | 1 | 1 | 1 | 1 | 1 | ||
3 | 1 | 1 | 1 | 1 | 1 | 1 | 1 | 1 | 1 | ||
4 | 1 | 1 | 1 | 1 | 1 | 1 | 1 | 1 | 1 | ||
5 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | ||
6 | 1 | 1 | 1 | 1 | 1 | 1 | 1 | 1 | 1 | ||
7 | 1 | 1 | 1 | 1 | 1 | 1 | 1 | 1 | 1 | ||
8 | 1 | 1 | 1 | 1 | 1 | 1 | 1 | 1 | 1 | ||
9 | 1 | 1 | 1 | 1 | 1 | 1 | 1 | 1 | 1 | ||
10 | 1 | 1 | 1 | 1 | 1 | 1 | 1 | 1 | 1 | ||
4.5 |
1 | 1 | 1 | 1 | 1 | 1 | 1 | 1 | 1 | 1 | Total parent animals 9 |
2 | 1 | 1 | 1 | 1 | 1 | 1 | 1 | 1 | 1 | ||
3 | 1 | 1 | 1 | 1 | 1 | 1 | 1 | 1 | 1 | ||
4 | 1 | 1 | 1 | 1 | 1 | 1 | 1 | 1 | 1 | ||
5 | 1 | 1 | 1 | 1 | 1 | 1 | 1 | 1 | 1 | ||
6 | 1 | 1 | 1 | 1 | 1 | 1 | 1 | 1 | 1 | ||
7 | 1 | 1 | 1 | 1 | 1 | 1 | 1 | 1 | 1 | ||
8 | 1 | 1 | 1 | 1 | 1 | 1 | 1 | 1 | 1 | ||
9 | 1 | 1 | 1 | 1 | 1 | 1 | 1 | 1 | 1 | ||
10 | 1 | 1 | 1 | 1 | 1 | 1 | 1 | 0 | 0 |
Table #2: Number of living offspring
conc. [mg/L] | parallel | 21 day total | 21 day mean |
control | 1 | 89 | mean value offspring per parent animal 74 |
2 | 61 | ||
3 | 63 | ||
4 | 88 | ||
5 | - | ||
6 | 84 | ||
7 | 69 | ||
8 | 76 | ||
9 | 70 | ||
10 | 66 | ||
4.5 | 1 | 80 | mean value offspring per parent animal 78 |
2 | 69 | ||
3 | 85 | ||
4 | 79 | ||
5 | 75 | ||
6 | 79 | ||
7 | 78 | ||
8 | 79 | ||
9 | 80 | ||
10 | - |
Description of key information
Long-term toxicity to Daphnia: NOEC (21-d reproduction) - 4.04 mg/L; EC50 (21-d reproduction) - 10.4 mg/L
Key value for chemical safety assessment
Fresh water invertebrates
Fresh water invertebrates
- Dose descriptor:
- NOEC
- Effect concentration:
- 4.04 mg/L
Additional information
Chronic toxicity to Daphnia has been investigated in a 21-day reproduction test according to OECD test methods and using dissolution/dispersion agents to increase concentration of the substance in the test medium. The NOEC (21-day, reproduction) was 4.04 mg/L and the EC50 (21 -day, reproduction) was 10.4 mg/L.
Tests with a structural analogue of the substance, a trimellitate ester with mixed C8-C10 side chains, showed no toxicity when tested at a concentration of 4.5 mg/L, this finding being in agreements with the observed NOEC/LOEC from testing with the substance itself. This lower value compared with the first study reflect the achieved concentrations of the substance in the test media, this influenced by the agents used to achieve dissolution/dispersion.
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