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Ecotoxicological information

Toxicity to soil macroorganisms except arthropods

Administrative data

Endpoint:
toxicity to soil macroorganisms except arthropods: short-term
Type of information:
experimental study
Adequacy of study:
key study
Study period:
10.08.2018 - 17.04.2019
Reliability:
1 (reliable without restriction)

Data source

Reference
Reference Type:
study report
Title:
Unnamed
Year:
2019
Report date:
2019

Materials and methods

Test guideline
Qualifier:
according to guideline
Guideline:
OECD Guideline 207 (Earthworm, Acute Toxicity Tests)
GLP compliance:
yes

Test material

Reference
Name:
Unnamed
Type:
Constituent

Sampling and analysis

Analytical monitoring:
no

Test substrate

Vehicle:
no

Test organisms

Test organisms (species):
Eisenia fetida
Animal group:
annelids
Details on test organisms:
Earthworm (Eiseniafoetida) was selected as a test species because it is recommended by MEP Guideline for Testing of Chemicals N o.207.
The test earthwonn (Eisenia ji:Jetida) was obtained from Chenggong Earthworm Raising Farm in Dongli District, Tianjin. The batch number was earthworm20170626.
The domestication conditions of earthworms were 20±2°C, 80±15% relative humidity and continuous light.The adult (at least two months old with clitellum) worms with an individual weight of 0.3000~0.5635 g were used in the test.

Study design

Study type:
laboratory study
Substrate type:
artificial soil
Limit test:
yes
Total exposure duration:
14 d

Test conditions

Test temperature:
19.3~20.6 °C
Moisture:
66~89 % relative humidity
Details on test conditions:
During the test, monitored temperature was in the range of 19.3~20.6 °C, continuous light in condition of 66~89 % relative humidity monitored content, light intensity was in the range of 543- 679 Lux.
Nominal and measured concentrations:
I 000 mg/kg dry soil weight

Results and discussion

Effect concentrationsopen allclose all
Key result
Duration:
7 d
Dose descriptor:
LC50
Effect conc.:
> 1 000 mg/kg soil dw
Nominal / measured:
nominal
Conc. based on:
test mat.
Basis for effect:
mortality
Key result
Duration:
14 d
Dose descriptor:
LC50
Effect conc.:
> 1 000 mg/kg soil dw
Nominal / measured:
nominal
Conc. based on:
test mat.
Basis for effect:
mortality
Details on results:
At the end of test, there was no difference in earthworm weight reduce rate between the test and control group. The moisture content was 30.0% and 30.0% for control and test medium at the beginning of the test and was in the range of28.8%-29.6% and 29.3-29.8% for control and test medium at the end of the test.
The pH value was 5.51 and 4.92 for control and test medium, respectively.
During the exposure period, there was no mortality or abnormal signs observed at control and 1000 mg/kg dry soil weight test group.

Applicant's summary and conclusion

Validity criteria fulfilled:
yes
Conclusions:
No mortality of the test organisms was observed during the test. According to the results, the acute toxicity of Sa 190 was:
LC50(7 d): > 1000mg/kg dry soil weight
LC50(14 d): > 1000mg/kg dry soil weight
Executive summary:

No mortality of the test organisms was observed during the test. According to the results, the acute toxicity of Sa 190 was:

LC50(7 d): > 1000mg/kg dry soil weight

LC50(14 d): > 1000mg/kg dry soil weight