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Adsorption / desorption

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Reference
Endpoint:
adsorption / desorption: screening
Type of information:
experimental study
Adequacy of study:
key study
Reliability:
1 (reliable without restriction)
Rationale for reliability incl. deficiencies:
guideline study
Qualifier:
according to
Guideline:
OECD Guideline 121 (Estimation of the Adsorption Coefficient (Koc) on Soil and on Sewage Sludge using High Performance Liquid Chromatography (HPLC))
Version / remarks:
2001
Qualifier:
according to
Guideline:
EU Method C.19 (Estimation of the Adsorption Coefficient (KOC) on Soil and Sewage Sludge Using High Performance Liquid Chromatography (HPLC))
Version / remarks:
2008
GLP compliance:
yes
Type of method:
HPLC estimation method
Media:
soil/sewage sludge
Radiolabelling:
no
Test temperature:
30 °C
Details on study design: HPLC method:
EQUIPMENT
- Apparatus: 515 HPLC Pump, 717 Plus Autosampler (Waters, Milford, MA, USA)
- Type, material and dimension of analytical (guard) column: Hypersil BDS-CN, 150 x 4.6 mm i.d., dp = 5 µm (Thermo Fisher Scientific, Waltham, MA, USA)
- Detection system: Photodiode Array Dectector 2996, UV dectection at 210 nm (Waters, Milford, MA, USA), Refractive Index Detector 2414, sensitivity = 4, time constant = 1 sec (Waters, Milford, MA, USA)


MOBILE PHASES
- Type: 55/45 (v/v) Methanol/Water
- Experiments with additives carried out on separate columns: no
- Solutes for dissolving test and reference substances: Methanol

REFERENCE SUBSTANCES
- Identity: Acetanilide

DETERMINATION OF RETENTION TIMES
- Quantity of test substance introduced in the column: 10 µl
- Quantity of reference substances: 10 µl

REPETITIONS
- Number of determinations: 2

Type:
Koc
Value:
< 18
Type:
log Koc
Value:
< 1.26
Details on results (HPLC method):
UV detection
- Retention times of reference substances used for calibration: Acetanilide: 2.23 min (Log Koc: 1.26)
- Retention time of test Substance: 1.91 min
RI detection
- Retention times of reference substances used for calibration: Acetanilide: 2.46 min (Log Koc: 1.26)
- Retention time of test Substance: 2.14 min

It was concluded that the log Koc of the test substance was <1.26 (Koc <18)

Description of key information

log Koc <1.26; OECD 121

Key value for chemical safety assessment

Additional information

The adsorption coefficient (Koc) of 2,3,3,3-Tetrafluoropropene was determined in a study following OECD 121 (Daikin Industries Ltd., 2010). The adsorption of the substance on soil and sewage sludge was estimated by HPLC. The retention time of the test substance was compared to values obtained for the reference substance acetanilide. A Koc <18 and a log Koc <1.26 was determined.