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REACH

Biphenyl-4,4'-diol

EC number: 202-200-5 | CAS number: 92-88-6

Life Cycle description
Uses advised against

Ecotoxicological information

Short-term toxicity to aquatic invertebrates

Currently viewing:

Administrative data

Link to relevant study record(s)

Reference

Endpoint:

short-term toxicity to aquatic invertebrates

Type of information:

experimental study

Adequacy of study:

key study

Reliability:

1 (reliable without restriction)

Rationale for reliability incl. deficiencies:

comparable to guideline study

Remarks:

Conducted to OECD test guideline. GLP study with chemical analysis by HPLC

Qualifier:

according to guideline

Guideline:

OECD Guideline 202 (Daphnia sp. Acute Immobilisation Test)

Deviations:

GLP compliance:

yes

Specific details on test material used for the study:

Biphenyl-4,4’-diol / CAS 92-88-6

Analytical monitoring:

yes

Details on sampling:

Samples of prepared test medium were taken at 0 hours (fresh) and 24 hours (old)

Vehicle:

yes

Details on test solutions:

Test solution prepared by dissolution of weighed quantities of test substance into solvent dimethyl formamide (DMF) followed by dilution in Elendt M4 medum at a volume of 100 uL/L

Test organisms (species):

Daphnia magna

Details on test organisms:

Test organisms were supplied from an internal laboratory culture that was fed chlorella vulgaris (0.2 mg Carbon)

Test type:

semi-static

Water media type:

freshwater

Limit test:

Total exposure duration:

48 h

Post exposure observation period:

Hardness:

244 to 248 (0-24 hours)

Test temperature:

19.9 to 20.1°C

pH:

8.3 to 8.5

Dissolved oxygen:

8.5 to 8.8 mg/L

Salinity:

Nominal and measured concentrations:

Nominal concentrations of 0.5, 0.85, 1.4, 2.4, 4.1 and 7.0 mg/L
Measured concentrations: 0.513, 0.86, 1.44, 2.48, 4.17 and 7.25 mg/L

Details on test conditions:

A 48 hour acute toxicity test was conducted in accordance with OECD 202 Acute toxicity to Daphnia magna, under semi-static conditions. Based on the results of a range finding test a definitive test was conducted at nominal concentration of 0.5, 0.85, 1.4, 2.4, 4.1 and 7.0 mg/L. Four
replicate test vessels were prepared per test concentration containing 100 mL of test medium and 5 Daphnia per vessel (20 per concentration in
total).

The test was conducted with a 16 hour light and 8 hour dark lighting regime at (ca. 800 Lux).

Test solutions were prepared by direct weighing and dilution in a solvent vehicle (DMF) prior to dilution (100 µL/L) in Elendt M4 medium.

Concentrations were confirmed at 0 and 24 hours, 103 to 106% and 99 to 102% respectively (LOD 0.0002 mg/L).

Reference substance (positive control):

not specified

Duration:

24 h

Dose descriptor:

EC50

Effect conc.:

> 7.25 mg/L

Nominal / measured:

meas. (geom. mean)

Conc. based on:

test mat.

Basis for effect:

mobility

Duration:

24 h

Dose descriptor:

EC50

Effect conc.:

0.86 mg/L

Nominal / measured:

meas. (geom. mean)

Conc. based on:

test mat.

Basis for effect:

mobility

Duration:

24 h

Dose descriptor:

LC100

Effect conc.:

> 7.25 mg/L

Nominal / measured:

meas. (geom. mean)

Conc. based on:

test mat.

Basis for effect:

mobility

Key result

Duration:

48 h

Dose descriptor:

EC50

Effect conc.:

1.76 mg/L

Nominal / measured:

meas. (geom. mean)

Conc. based on:

test mat.

Basis for effect:

mobility

Remarks on result:

other: between 1.47 and 2.11

Key result

Duration:

48 h

Dose descriptor:

NOEC

Effect conc.:

0.513 mg/L

Nominal / measured:

meas. (geom. mean)

Conc. based on:

test mat.

Basis for effect:

mobility

Duration:

48 h

Dose descriptor:

EC100

Effect conc.:

7.25 mg/L

Nominal / measured:

meas. (geom. mean)

Conc. based on:

test mat.

Basis for effect:

mobility

Details on results:

There was no control or solvent control immobility during the conduct of the test

Results with reference substance (positive control):

Reported statistics and error estimates:

Probit

Immobility results

Nominal concentration (mg/L)	Geometric mean measured concentration (mg/L)	% Immobility
Nominal concentration (mg/L)	Geometric mean measured concentration (mg/L)	24 hours	48 hours
Control	-	0	0
Solvent control	-	0	0
0.5	0.513	0	0
0.85	0.860	0	5
1.4	1.44	10	50
2.4	2.48	10	65
4.1	4.17	20	95
7.0	7.25	25	100

Analytical results

Nominal concentration (mg/L)	Measured concentrations (mg/L)		Geometric mean measured concentrations (mg/L)
Nominal concentration (mg/L)	0 hours	24 hours	Geometric mean measured concentrations (mg/L)
Control	<LOD	<LOD	<LOD
Solvent control	<LOD	<LOD	<LOD
0.5	0.52	0.506	0.513
0.85	0.879	0.842	0.86
1.4	1.47	1.42	1.44
2.4	2.54	2.43	2.48
4.1	4.21	4.14	4.17
7	7.35	7.15	7.25

Validity criteria fulfilled:

yes

Conclusions:

The 48 hour EC50 was calculated to be 1.76 mg/L
The 48 hour NOEC was considered to be 0.860 mg/L

Executive summary:

A GLP 48 hour semi-static acute toxicity test was conducted with 4,4 -biphenol in accordance with OECD 202 Acute toxicity to Daphnia magna, under semi-static conditions using Elendt M4. Based on the results of a range finding test a definitive test was conducted at nominal concentration of 0.5, 0.85, 1.4, 2.4, 4.1 and 7.0 mg/L.

Geometric mean measured concentrations were: 0.513, 0.86, 1.44, 2.48, 4.17 and 7.25 mg/L.

The 48 hour EC₅₀was calculated to be 1.76 mg/L (based on geometric mean measured concentrations)

The 48 hour NOEC was considered to be 0.860 mg/L (based on geometric mean measured concentrations)

Description of key information

Nominal concentrations: 3.0, 5.3, 9.5, 17 and 30 mg/L (spacing factor 1.8)
Measured concentrations: 101-105% (0 hours), 99-106% (24 hours) analysis by HPLC.
96 hour EC50 = 1.76 mg/L (CI 95% 1.47 to 2.11 mg/L)

Key value for chemical safety assessment

Fresh water invertebrates

Effect concentration:: 1.76 mg/L

Additional information

A GLP 48 hour semi-static acute toxicity test was conducted with biphenyl-4,4'-diol in accordance with OECD 202 Acute toxicity to Daphnia magna, under semi-static conditions using Elendt M4. Based on the results of a range finding test a definitive test was conducted at nominal concentration of 0.5, 0.85, 1.4, 2.4, 4.1 and 7.0 mg/L.

Geometric mean measured concentrations were: 0.513, 0.86, 1.44, 2.48, 4.17 and 7.25 mg/L.

The 48 hour EC₅₀was calculated to be 1.76 mg/L (based on geometric mean measured concentrations)

The 48 hour NOEC was considered to be 0.860 mg/L (based on geometric mean measured concentrations)

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