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EC number: 265-157-1 | CAS number: 64742-54-7 A complex combination of hydrocarbons obtained by treating a petroleum fraction with hydrogen in the presence of a catalyst. It consists of hydrocarbons having carbon numbers predominantly in the range of C20 through C50 and produces a finished oil of at least 100 SUS at 100°F (19cSt at 40°C). It contains a relatively large proportion of saturated hydrocarbons.
- Life Cycle description
- Uses advised against
- Endpoint summary
- Appearance / physical state / colour
- Melting point / freezing point
- Boiling point
- Density
- Particle size distribution (Granulometry)
- Vapour pressure
- Partition coefficient
- Water solubility
- Solubility in organic solvents / fat solubility
- Surface tension
- Flash point
- Auto flammability
- Flammability
- Explosiveness
- Oxidising properties
- Oxidation reduction potential
- Stability in organic solvents and identity of relevant degradation products
- Storage stability and reactivity towards container material
- Stability: thermal, sunlight, metals
- pH
- Dissociation constant
- Viscosity
- Additional physico-chemical information
- Additional physico-chemical properties of nanomaterials
- Nanomaterial agglomeration / aggregation
- Nanomaterial crystalline phase
- Nanomaterial crystallite and grain size
- Nanomaterial aspect ratio / shape
- Nanomaterial specific surface area
- Nanomaterial Zeta potential
- Nanomaterial surface chemistry
- Nanomaterial dustiness
- Nanomaterial porosity
- Nanomaterial pour density
- Nanomaterial photocatalytic activity
- Nanomaterial radical formation potential
- Nanomaterial catalytic activity
- Endpoint summary
- Stability
- Biodegradation
- Bioaccumulation
- Transport and distribution
- Environmental data
- Additional information on environmental fate and behaviour
- Ecotoxicological Summary
- Aquatic toxicity
- Endpoint summary
- Short-term toxicity to fish
- Long-term toxicity to fish
- Short-term toxicity to aquatic invertebrates
- Long-term toxicity to aquatic invertebrates
- Toxicity to aquatic algae and cyanobacteria
- Toxicity to aquatic plants other than algae
- Toxicity to microorganisms
- Endocrine disrupter testing in aquatic vertebrates – in vivo
- Toxicity to other aquatic organisms
- Sediment toxicity
- Terrestrial toxicity
- Biological effects monitoring
- Biotransformation and kinetics
- Additional ecotoxological information
- Toxicological Summary
- Toxicokinetics, metabolism and distribution
- Acute Toxicity
- Irritation / corrosion
- Sensitisation
- Repeated dose toxicity
- Genetic toxicity
- Carcinogenicity
- Toxicity to reproduction
- Specific investigations
- Exposure related observations in humans
- Toxic effects on livestock and pets
- Additional toxicological data
![](https://echa.europa.eu/o/diss-blank-theme/images/factsheets/A-REACH/factsheet/print_toxicological-information.png)
Acute Toxicity: inhalation
Administrative data
- Endpoint:
- acute toxicity: inhalation
- Type of information:
- migrated information: read-across based on grouping of substances (category approach)
- Adequacy of study:
- key study
- Reliability:
- 1 (reliable without restriction)
- Rationale for reliability incl. deficiencies:
- other: This study is classified as reliable with out restriction because it was conducted according to OECD guideline 403.
Data source
Reference
- Reference Type:
- study report
- Title:
- Unnamed
- Year:
- 1 987
- Report date:
- 1987
Materials and methods
Test guideline
- Qualifier:
- equivalent or similar to guideline
- Guideline:
- OECD Guideline 403 (Acute Inhalation Toxicity)
- GLP compliance:
- yes
- Test type:
- standard acute method
- Limit test:
- yes
Test material
- Reference substance name:
- 64742-53-6 (> 3% IP346)
- IUPAC Name:
- 64742-53-6 (> 3% IP346)
- Reference substance name:
- hydrotreated light naphthenic distillate, insufficiently refined, IP 346 > 3%
- IUPAC Name:
- hydrotreated light naphthenic distillate, insufficiently refined, IP 346 > 3%
- Test material form:
- other: Oily liquid
- Details on test material:
- - Name of test material (as cited in study report): hydrotreated light naphthenic distillate (API 83-12)
- CAS number: 64742-53-6
- Substance type: lubricant base oil (insufficently refined IP346>3%)
- Storage condition of test material: stored at 4°C
- Physical state: liquid
- Viscosity, SSU:
53.5 at 100°F
33.3 at 210°F
- API Gravity: 26.2
- Flash Point: 255 °F
- Distillation range (°F) ASTM D86 Equiv: 533-713 (10-95%)
Initial Boiling Point (°F): 464
Final Boiling Point (°F): 796
- Pour Point (°F): 60
- Aniline Point (°F): 148.3
- Colour ASTM: 0.5
- Composition of test material ASTM D-2007, Wt. %:
Saturates: 61.6
Aromatics 36.1
Polar compounds: 2.3
Sulfur, Wt%: 0.019
Constituent 1
Constituent 2
Test animals
- Species:
- rat
- Strain:
- Sprague-Dawley
- Sex:
- male/female
- Details on test animals or test system and environmental conditions:
- TEST ANIMALS
- Source: Charles River Breeding Laboratories, Inc.
- Age at study initiation: 9 weeks (63 days)
- Weight at study initiation: males 292-310 grams, females 186-209 grams
- Fasting period before study: no
- Housing: individually housed in stainless steel hanging wire cages
- Diet (e.g. ad libitum): supplied Purina Certified Laboratory Chow # 5002 ad libitum
- Water (e.g. ad libitum): supplied water ad libitum
- Acclimation period: fourteen days
ENVIRONMENTAL CONDITIONS
- Photoperiod (hrs dark / hrs light): 12 hours light/12 hours dark
Administration / exposure
- Route of administration:
- inhalation: aerosol
- Type of inhalation exposure:
- whole body
- Vehicle:
- other: Air
- Details on inhalation exposure:
- GENERATION OF TEST ATMOSPHERE / CHAMBER DESCRIPTION
- Exposure apparatus: rats were individually housed in stainless steel and glass chambers
- Exposure chamber volume: 0.25 cubic meters
- Source and rate of air: the total air flow through the chamber was maintained at 2.0 cfm as measured by a calibrated orifice meter
- System of generating particulates/aerosols: aerosols were generated using a DeVilbiss No. 40 nebulizer
- Method of particle size determination: determined during each exposure by a Anderson Model 2000 cascade impactor
- Temperature and humidity chamber: temperature ranged from 23 to 25°C, and 32 to 70% humidity
TEST ATMOSPHERE
- Brief description of analytical method used: analytical samples were taken at least once per hour during each exposure by drawing a known volume of test atmosphere through tared Gelman A/E glass fiber filters. By knowing the amount of test material on the filter and the volume of air sampled the analytical exposure concentration was determined.
VEHICLE
air
GENERATION OF TEST ATMOSPHERE / CHAMBER DESCRIPTION
- Exposure apparatus:
- Exposure chamber volume:
- Method of holding animals in test chamber:
- Source and rate of air:
- Method of conditioning air:
- System of generating particulates/aerosols:
- Method of particle size determination:
- Treatment of exhaust air:
- Temperature, humidity, pressure in air chamber:
TEST ATMOSPHERE
- Brief description of analytical method used:
- Samples taken from breathing zone: yes/no
VEHICLE
- Composition of vehicle (if applicable):
- Concentration of test material in vehicle (if applicable):
- Justification of choice of vehicle:
- Lot/batch no. (if required):
- Purity:
TEST ATMOSPHERE (if not tabulated)
- Particle size distribution:
- MMAD (Mass median aerodynamic diameter) / GSD (Geometric st. dev.): ranges of 1.70 to 2.5 microns/1.50 to 1.61 microns
CLASS METHOD (if applicable)
- Rationale for the selection of the starting concentration: - Analytical verification of test atmosphere concentrations:
- yes
- Duration of exposure:
- 4 h
- Concentrations:
- Phase 1, limit test: 5 mg/L
Phase 2: 0, 1.0, 1.5, 2.5, 3.5 (actual concentrations: 0, 0.02, 1.04, 1.51, 2.37, 3.49) - No. of animals per sex per dose:
- 5 males and 5 females
- Control animals:
- yes
- Details on study design:
- - Duration of observation period following administration: 14 days
- Frequency of observations and weighing: test animals were observed continuously for the first hour, hourly for the duration of the exposure, and once daily for the fourteen day post-exposure period. Weights were recorded prior to exposure and again on days 7 and 14 of the observation period.
- Necropsy of survivors performed: yes
- Other examinations performed: clinical signs, body weight, gross necropsy, and histopathological examination
Results and discussion
- Preliminary study:
- Five male and five female rate were exposed to a concentration of 5 mg/L for four hours. All animals in this test died.
Effect levels
- Sex:
- male/female
- Dose descriptor:
- LC50
- Effect level:
- 2.18 mg/L air
- 95% CL:
- 1.8 - 2.55
- Exp. duration:
- 4 h
- Mortality:
- No animals died in the control group. One male and one female died at a concentration of 1.0 mg/L, no animals died at a concentration of 1.5 mg/L, three males and three females died at a concentration of 2.5 mg/L, and all animals died at a concentration of 3.5.
- Clinical signs:
- other: see Other findings
- Body weight:
- No consistent dose related effect on body weight was observed
- Gross pathology:
- Animals exposed to concentrations of 5.0 and 3.5 mg/L consistently exhibited dark red lungs. They were also observed in three males and three females at 2.5 mg/L, one male and two females at 1.5 mg/L, and in one male and one female at 1.0 mg/L. This observation was present in all animals who died within a day or two of exposure.
A single skin lesion (scab) was observed in one male at 1.5 mg/L. - Other findings:
- The following clinical signs were observed: decreased activity, wet inguinal area, eyes partially closed, wet coat, loose stool, and oily coat. During the first week post-exposure all of these signs were exhibited in addition to signs of poor-condition, respiratory distress and death were observed. During the second week most survivors were considered of normal appearance.
In histopathology observations affected animals presented moderate to marked diffuse pulmonary congestion and perivascular oedema, damage to alveolar walls. Necrosis and inflammation were seen in the walls of small blood vessels and there was spotty epithelial necrosis in small bronchioles.
Any other information on results incl. tables
Actual
exposure concentrations and mortalities were as follows:
Target level |
Actual concentration |
Mortality |
||
(mg/l) |
mg/l |
±SD |
Male |
Female |
0 |
0.02 |
0.01 |
0/5 |
0/5 |
1.0 |
1.04 |
0.1 |
1/5 |
1/5 |
1.5 |
1.51 |
0.15 |
0/5 |
0/5 |
2.5 |
2.37 |
0.31 |
3/5 |
3/5 |
3.5 |
3.49 |
0.36 |
5/5 |
5/5 |
5.0 |
5.05 |
0.18 |
5/5 |
5/5 |
Particle size measurements confirmed
that mass median aerodynamic
diameter
and geometric standard deviation values were in the ranges 1.7
to
2.5 µm and 1.5 to 1.61 respectively. These
measurements confirm that the
particles were within the respirable range.
The LC50 for combined sexes was estimated to be 2.18 with 95% confidence
limits
of 1.80 to 2.55 mg/l.
Body weight differences did not show a consistent dose related pattern.
At the highest concentration, the animals were obscured by a dense
aerosol
and observations could not be made during the exposure period. In
other
groups, there was a decreased activity, wet inguinal area, eyes
partially
closed, wet coat, loose stool and oily coat during exposure.
During the first week post-exposure, similar signs were observed as well
as
signs of poor condition, respiratory distress and some deaths
occurred.
During
test week 2, most survivors were considered to be of normal
appearance. The
signs that were observed occurred in a dose related
manner.
At gross necropsy, dark red lungs were described for some animals. The
incidence
is shown below.
Dose Group |
Male |
Female |
0 |
0/5 |
0/5 |
1.0 |
1/5 |
1/5 |
1.5 |
0/5 |
0/5 |
2.5 |
3/5 |
3/5 |
3.5 |
5/5 |
5/5 |
5.0 |
5/5 |
5/5 |
At histology, affected animals
exhibited diffuse pulmonary congestion and
perivascular
oedema that were mostly moderate or marked in degree. Less consistently
spotty alveolar edema was also seen. There was widespread damage
to alveolar walls resulting in fibronecrotic debris resembling
hyaline
membranes in more marked cases and extravasation of RBCs and
PMNs.
Necrosis and inflammation were seen in the walls of small blood
vessels
and there was spotty epithelial necrosis in small bronchioles,
but
the most severe damage seemed to be centroacinar. The larger airways
were
relatively unaffected.
None of the surviving animals exhibited the above acute changes.
However,
most of the surviving animals exposed to 2.5 or 1.0 mg/l and
above
exhibited chronic inflammatory changes that were not seen in the
controls
and only occasionally in animals exposed at the 1.5 mg/l level,
and
then to a lesser degree of severity. Other findings were considered
sporadic or unrelated to exposure to the test material.
Applicant's summary and conclusion
- Interpretation of results:
- Toxicity Category IV
- Remarks:
- Migrated information Criteria used for interpretation of results: EU
- Conclusions:
- The LC50 for males and females was 2.18 mg/L with 95% confidence limits at 1.80 to 2.55 mg/L for insufficiently refined lubricant base oil.
- Executive summary:
A group of five male and five female rats were exposed for 4 hours to an aerosol of the test material at a target concentration of 5 mg/L. Four additional groups of rats were then exposed for 4 hours to target aerosol concentrations of 1, 1.5, 2.5 and 3.5 mg/L. A control group exposed, in the chamber, to air only was also included. Animals were observed continuously during the first hour of exposure, hourly for the remainder of the exposure and once daily for the 14-day post exposure period. Mortalities were recorded and body weights were measured prior to exposure and again 7 and 14 days after exposure. On the 14th day post-exposure, necropsies were performed on all surviving animals. For all animals, including animals found dead, the lungs and any other abnormal tissues were removed and fixed for subsequent histopathological examination.
At 5.0 mg/L, the animals were obscured by a dense aerosol and observations could not be made during the exposure period. In other groups, there was a decreased activity, wet inguinal area, eyes partially closed, wet coat, loose stool and oily coat during exposure. During the first week post-exposure, similar signs were observed as well as signs of poor condition, respiratory distress and some deaths occurred. During test week 2, most survivors were considered to be of normal appearance. The signs that were observed occurred in a dose related manner. At gross necropsy, dark red lungs were described for some animals. No animals died in the control group. One male and one female died at a concentration of 1.0 mg/L, no animals died at a concentration of 1.5 mg/L, three males and three females died at a concentration of 2.5 mg/L, and all animals died at a concentration of 3.5 mg/L.
The LC50 for males and females was 2.18 mg/L with 95% confidence limits at 1.80 to 2.55 mg/L for insufficiently refined lubricant base oil.
This study received a Klimisch score of 1 and is classified as reliable without restriction because it was conducted according to OECD guideline 403.
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