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EC number: 931-597-4
CAS number: -
Algal cell particle densities (cell particles per unit volume)
were measured as a surrogate for biomass. The algal cell particle
densities measured at each time period are given in Table 2. The means
of these values are also shown in Table 2 and are plotted as growth
curves in Figure 1.
Table 2 Algal cell densities
3 Mean yields over the test period
* Significant difference (P=0.05) from the control
The growth rate (0 to72hours)
was calculated for each replicate culture, according to the formula.
Growth rate = (Logn(N2/N1))/t
N1 = Cell density at start of test
N2 = Celldensity
at end of test
t = Time interval (days)
These data were analysed as described for the yield method. The mean
growth rates,are given in Table 4, together with the rates expressed as
percentages of the control.
A Weibull model was fitted to the percent effect and log concentration
data. This model was then used to estimate the median effective
concentration (based on yield curve area, EyC50) and its 95%
Table 4 Mean growth rates over the test period
Table 5 Test solution pH and salinity measurements
Short-term toxicity of Ash toward a green alga Pseudokirchneriella
subcapitata was studied in a GLP compliant laboratory according to
the OECD Guideline 201 (Alga, Growth Inhibition Test). The 72 h test was
performed at six concentrations of filtered Ash solutions with three
replicates in constant illumination and at temperature of 22 ± 2°C. The
nominal cell density was 0.5 x 104 cells. The growth curve
for control was exponential. In the test, significant effects (p = 0.05)
on alga growth was observed. The effect concentrations for yield and
growth were found to be EyC50 = 8.5 mg/L and ErC50 = 21.3 mg/L,
Toxicity of Ash to Green alga Pseudokirchneriella subcapitata was determined in a GLP compliant laboratory study according to OECD Guideline 201 (Alga, Growth Inhibition Test ). The EC50 (72h) for growth rate was 21.3 mg/L and for yield it was 8.5 mg/L.
Due to low water solubility of Ash, Water Accommondated Fractions of the
test solutions were prepared.
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