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EC number: 931-597-4
CAS number: -
Table 4 Daphnia magna response
Physical and chemical data
Dissolved oxygen concentrations ranged from 6.2 to 9.2 mg/L (68 to 101%
of the air saturation value), pH values (after time 0 adjustments)
ranged from 7.1 to 8.2, and the salinity ranged from 0.2 to 0.3‰.
Individual data are shown in Table 5.
Table 5 Dissolved oxygen concentrations and pH values
a Measurements taken at 24 hours, as 100% mortalities at that time
Temperature values taken at 0, 24 and 48 hours were 21.6, 21.4 and
The hardness of the D. magna media was 181 mg/L CaCO3, and the
conductivity was 679 µS/cm. Water quality parameters of a recent batch
of D. magna media are shown in Table 6.
Table 6 Dilution water parameters
*Conductivity @ 25ºC
679 µS cm-1
*Total hardness as CaCO3
181 mg L-1
*Alkalinity as CaCO3
34 mg L-1
Solids suspended @105ºC
<3 mg L-1
Dissolved organic carbon
0.210 mg L-1
Chemical oxygen demand
Ammoniacal nitrogen as N
<0.002 mg L-1
Highest organophosphorous pesticides
<0.01 µg L-1
Highest organochlorine pesticides
0.1 µg L-1
<0.001 µg L-1
<10 µg L-1
<1 µg L-1
527 µg L-1
<0.1 µg L-1
<0.5 µg L-1
2.30 µg L-1
5.32 µg L-1
74.0 µg L-1
<2 µg L-1
<5 µg L-1
*Analyses conducted at Brixham Environmental Laboratory (BEL) on the
batch of media used in the study.Other analyses were performed on
a recent batch of media by The Environment Agency (EA), National
Laboratory Service (NLS), Olympia House, Gelderd Lane, Gelderd Road,
Leeds, LS12 6DD, for analysis at NLS laboratories. The EA is a non-GLP
Toxicity of Ash to Daphnia magna was determined in a GLP compliant laboratory study according to OECD Guideline 202 (Daphnia sp. Acute Immobilisation Test). The EC50 (48h) was found to be 57.1 mg/L.
Due to low water solubility of Ash, Water Accommondated Fractions of the
test solutions were prepared.
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