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EC number: 200-661-7
CAS number: 67-63-0
The substance has a BOD5/ThOD ratio of 0.50, and is therefore considered
to be readily degradable.
The BOD5 (non-adapted), BOD5 (adapted) and COD of the substance
were reported by Bridié et al. (1979) to be 1.19 g O2/g, 1.72 g O2/g and
2.23 g O2/g, respectively. The BOD test was conducted in accordance
with the standard dilution method (APHA "Standard Methods" No. 219
(1971)) at 20 ± 1ºC for a period of 5 days. The only deviation from the
APHA standard was the addition of 0.5 mg/l allylthiourea in each test to
prevent nitrification. 500 ml test solutions were seeded with a filtered
10 ml volume of the effluent from a biological sanitary waste treatment
plant. The authors reported that in some cases an adapted seed was
prepared and used, although in no case was inducement of adaptation
tried exhaustively. Duplicate tests were run on a mixture of glucose and
glutamic acid, as recommended in the APHA method, as a means of checking
the activity of the inoculum. The COD test was conducted in accordance
with the standard potassium dichromate method described in ASTM D 1252
-97 (reapproved 1974). The BOD5/COD ratios from these results are 0.53
(non-adapted) and 0.77 (adapted). As indicated in the REACH Endpoint
Specific Guidance section R.22.214.171.124; where no other measured
degradability data are available, BOD5 data can be used for
classification purposes, but where the chemical structure is known, a
calculated theoretical oxygen demand (ThOD) value should be used instead
of the COD. According to Annex IV of OECD 301, the ThOD of this
substance can be calculated to be 2.40 g O2/g, which gives BOD5/ThOD
ratios of 0.50 (non-adapted) and 0.72 (adapted). The value for
non-adapted seed equals 0.5, at which level or above a substance can be
considered to be readily biodegradable.
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