Registration Dossier
Registration Dossier
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EC number: 200-539-3 | CAS number: 62-53-3
Skin sensitisation
Administrative data
- Endpoint:
- skin sensitisation: in vivo (LLNA)
- Type of information:
- experimental study
- Adequacy of study:
- weight of evidence
- Reliability:
- 2 (reliable with restrictions)
- Rationale for reliability incl. deficiencies:
- other: Comparable to guideline study with acceptable restrictions (limited reporting).
Data source
Referenceopen allclose all
- Reference Type:
- publication
- Title:
- Comparison of the local lymph node assay with the guinea-pig maximization test for the detection of a range of contact allergens
- Author:
- Basketter DA, Scholes EW
- Year:
- 1 992
- Bibliographic source:
- Food Chem. Toxicol. 30, 65-69
- Reference Type:
- publication
- Title:
- The performance of the local lymph node assay with chemicals identified as contact allergens in the human maximization test
- Author:
- Basketter DA, Scholes EW, Kimber I
- Year:
- 1 994
- Bibliographic source:
- Food Chem Toxicol. 32, 543-547
Materials and methods
Test guideline
- Qualifier:
- equivalent or similar to guideline
- Guideline:
- OECD Guideline 429 (Skin Sensitisation: Local Lymph Node Assay)
- Deviations:
- yes
- Remarks:
- limited reporting
- GLP compliance:
- no
- Type of study:
- mouse local lymph node assay (LLNA)
Test material
- Reference substance name:
- Aniline
- EC Number:
- 200-539-3
- EC Name:
- Aniline
- Cas Number:
- 62-53-3
- Molecular formula:
- C6H7N
- IUPAC Name:
- aniline
- Details on test material:
- Supplier: Aldrich Chemical Co., Gillingham, Dorset, UK
Purity: no data
Constituent 1
In vivo test system
Test animals
- Species:
- mouse
- Strain:
- CBA
- Sex:
- male/female
Study design: in vivo (LLNA)
- Vehicle:
- acetone/olive oil (4:1 v/v)
- Concentration:
- 10, 25, 50%
- No. of animals per dose:
- 4
Results and discussion
In vivo (LLNA)
Results
- Parameter:
- SI
- Remarks on result:
- other: 1.4, 1.8 and 2.9 for 10, 25, 50% aniline respectively; no EC3 value could be calculated since the highest tested concentration of 50% aniline did induce a stimulation index of below 3.
Any other information on results incl. tables
Applicant's summary and conclusion
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